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The map label for this gene is dnaB [H]

Identifier: 91783367

GI number: 91783367

Start: 2209738

End: 2211123

Strand: Direct

Name: dnaB [H]

Synonym: Bxe_A2452

Alternate gene names: 91783367

Gene position: 2209738-2211123 (Clockwise)

Preceding gene: 91783366

Following gene: 91783368

Centisome position: 45.14

GC content: 61.9

Gene sequence:

>1386_bases
ATGAACGCACCGTCCAAAGATCCCCAACTCGAGTCGCTGAAAGTCCCGCCGCATTCGATCGAGGCCGAGCAATCGGTGCT
GGGCGGCCTGCTGCTCGACAACGCCGCATGGGACCGCATTGCGGACTTTCTGTCGCAGAGCGATTTTTACCGCTATGACC
ACCGCATCATCTTCGAGCACATCGGCAAGCTGATCGCGGCTACGCGTCCGGCGGACGTGATCACGGTGTACGAGGCGCTC
GGCACCGCGGGCAAGGCGGAAGAGGTCGGCGGTCTCGCTTACCTGAATGCGCTGGCCCAGAACACGCCGAGCGCGGCGAA
TATCCGGCGCTACGCGGAAATCGTGCGCGACCGCGCGGTGCTGCGGCGGCTGGTTTCCGTCGCCGACGAAATCTCGGCCG
ACGCCTTCAATCCGCAGGGCAAGGAAGTCCGGCAGTTGCTGGACGAAGCCGAATCGAAGGTGTTTTCGATCGCCGAAGAC
GGCGCGCGCGGCACTCAGGGTTTCCTGGAAATCGGGCCGTTGCTGACCGAAGTCGTCGAGCGGATCGATACGCTGTATCA
CACGGCCAATCCTAGCGACGTGACCGGCACGCCGACCGGCTTCGTCGATCTGGACCGCATGACGTCCGGCATGCACGGCG
GCGAGCTGATCATCGTCGCGGGCCGTCCGTCGATGGGTAAGACGGCGTTTTCCATGAACATCGGCGAATACGTGGCAGTC
GAGTATGGTTTGCCGGTCGCGGTGTTCTCGATGGAAATGCCGGGTTCGCAGCTCACCATGCGTATGCTCGGCTCGGTCGG
GCGGCTGGATCAACACCGCATGCGAACCGGGCGCCTGACCGACGAGGATTGGCCGAAGCTCACGCACGCCGTGCAGAAAA
TGAGCGAGGCGCAGATTTTCATCGACGAAACCGGCGGCCTGAACCCGATGGAATTGCGCTCGCGGGCGCGCCGGTTGTCG
CGCCAGTGCGGCAAGCTGGGACTGATCATCATCGACTACCTGCAACTGATGAGCGGGTCGTCGTCAGGCGAAAACCGCGC
GACCGAAATCTCGGAAATCTCGCGCTCGCTGAAGAGTCTCGCGAAGGAACTCGACGTGCCGGTGATCGCTTTGTCGCAGC
TCAACCGGGGTCTCGAGCAGCGCCCGAACAAGCGTCCGATCATGTCGGACCTGCGCGAATCCGGCGCTATCGAACAGGAT
GCCGACGTGATCCTGTTTATTTACCGCGACGAAGTATACAACCCGGACAGCCCGGACAAAGGCACCGCCGAGATTATCAT
CGGCAAACAGCGGAACGGTCCGATCGGACCCGTTCGGCTCACGTTCCACGGTCAATTCACGAAGTTCGACAACTTTGCAG
GCGCACAGAATTTCTACAGCGATTGA

Upstream 100 bases:

>100_bases
GAAGGGCAGGGGCCGGGTGACCGGCCCCTGCCTTTTTTGTGTCCGCTTTTTAGTTTTTCGGCCGCCCGCGCGCCCTCGAT
TTGCCCGATAATTCCTCTCC

Downstream 100 bases:

>100_bases
GCGGAGAAGTGAAGCGATAACTGGGGCGCCGTTGTAACGGCGCTTTCAAAAGTGCGGCGCGGGTCTCGCGACGGTACAAT
GTGGCGGTTTTATGTCAGCC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEHIGKLIAATRPADVITVYEAL
GTAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAED
GARGTQGFLEIGPLLTEVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIFIDETGGLNPMELRSRARRLS
RQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQD
ADVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD

Sequences:

>Translated_461_residues
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEHIGKLIAATRPADVITVYEAL
GTAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAED
GARGTQGFLEIGPLLTEVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIFIDETGGLNPMELRSRARRLS
RQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQD
ADVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD
>Mature_461_residues
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEHIGKLIAATRPADVITVYEAL
GTAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAVLRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAED
GARGTQGFLEIGPLLTEVVERIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIFIDETGGLNPMELRSRARRLS
RQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSLAKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQD
ADVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=458, Percent_Identity=56.3318777292576, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50742; Mature: 50742

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEH
CCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IGKLIAATRPADVITVYEALGTAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAV
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGTQGFLEIGPLLTEVVE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
HHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEE
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIF
ECCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEE
IDETGGLNPMELRSRARRLSRQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQDADVILFIYRDEVYNPDSPDK
HHHCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
GTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD
CCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MNAPSKDPQLESLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFLSQSDFYRYDHRIIFEH
CCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IGKLIAATRPADVITVYEALGTAGKAEEVGGLAYLNALAQNTPSAANIRRYAEIVRDRAV
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRRLVSVADEISADAFNPQGKEVRQLLDEAESKVFSIAEDGARGTQGFLEIGPLLTEVVE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RIDTLYHTANPSDVTGTPTGFVDLDRMTSGMHGGELIIVAGRPSMGKTAFSMNIGEYVAV
HHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEE
EYGLPVAVFSMEMPGSQLTMRMLGSVGRLDQHRMRTGRLTDEDWPKLTHAVQKMSEAQIF
ECCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEE
IDETGGLNPMELRSRARRLSRQCGKLGLIIIDYLQLMSGSSSGENRATEISEISRSLKSL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AKELDVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQDADVILFIYRDEVYNPDSPDK
HHHCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
GTAEIIIGKQRNGPIGPVRLTFHGQFTKFDNFAGAQNFYSD
CCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]