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The map label for this gene is rlmB [H]

Identifier: 91783279

GI number: 91783279

Start: 2123543

End: 2124286

Strand: Direct

Name: rlmB [H]

Synonym: Bxe_A2539

Alternate gene names: 91783279

Gene position: 2123543-2124286 (Clockwise)

Preceding gene: 91783278

Following gene: 91783280

Centisome position: 43.37

GC content: 67.2

Gene sequence:

>744_bases
ATGGCACGTCTCAAGGTTTTATATGGTTTCCACGCAGTGACCGCGCGTATCCGGCACGATGCGTCGACAGTCGAAGATGT
TTACTACGACGCAACGCGCAAAGACCGTCGCATGACCGAATTCCTGCACGCGGCCAAAGAAGCCGGCGTGCGCCTGATCG
CCGCCGACGAAACGCGTCTGTGGGGCCTCGCGCACACCGAGCGCCACCAGGGCGTGGTCGCGCGCGCGGGCGACATGCCG
CTCGCGCAGAACCTGGCCGAGCTGCTCGACGGCATCAACGGTTCGCCGCTGATTCTCGTGCTCGACGGCGTGACCGATCC
GCACAACCTCGGCGCCTGCCTGCGCGTGGCCGATGCCGCCGGCGCGCACGCGGTGATCGCGCCGCGTGACCGCGCGGTAG
GTTTGAACGCGACGGCCGCGAAGGTGGCGAGCGGCGCGGCCGACACCGTGCCGTACATCACCGTGACGAATCTGGCGCGC
GCGCTGCGTGAGTTGAAGGATGCGGGCGTGTGGGTCGTCGGGACGGCCGGCGAGGCCACCAAAAGCCTCTACGACACGGA
GCTGGACGGTCCGGTGGCACTGGTGATGGGCGCCGAAGGCGAGGGCATGCGCCGCCTTACGCGCGACACCTGCGACGTGG
TCATGCAGATTCCGATGGCGGGCACGGTGGAAAGCCTGAATGTCTCGGTGGCGAGCGGCGTGTGTCTGTTCGAAGCCGTG
CGGCAGCGTTCGGTGAAGAAGTAA

Upstream 100 bases:

>100_bases
TCGAGCGCGGCAGACAAGCGCGGCGGACAGGTTCGCGCACATTCATATTCATCACAGCAGCGCATGTTGCACGCGCGCTC
AATCAAAGGTTGTATCAGTC

Downstream 100 bases:

>100_bases
GCCCGGCCGGCGCTCGCGCGCCACTCCGGTAAACTACCGTTTTTCTACTGCCCGCCAGCCTCTCCCATGACTCAAGACGA
ACTCAAACAACTGGTCGGCC

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MARLKVLYGFHAVTARIRHDASTVEDVYYDATRKDRRMTEFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDMP
LAQNLAELLDGINGSPLILVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVVGTAGEATKSLYDTELDGPVALVMGAEGEGMRRLTRDTCDVVMQIPMAGTVESLNVSVASGVCLFEAV
RQRSVKK

Sequences:

>Translated_247_residues
MARLKVLYGFHAVTARIRHDASTVEDVYYDATRKDRRMTEFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDMP
LAQNLAELLDGINGSPLILVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVVGTAGEATKSLYDTELDGPVALVMGAEGEGMRRLTRDTCDVVMQIPMAGTVESLNVSVASGVCLFEAV
RQRSVKK
>Mature_246_residues
ARLKVLYGFHAVTARIRHDASTVEDVYYDATRKDRRMTEFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARAGDMPL
AQNLAELLDGINGSPLILVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGVWVVGTAGEATKSLYDTELDGPVALVMGAEGEGMRRLTRDTCDVVMQIPMAGTVESLNVSVASGVCLFEAVR
QRSVKK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=261, Percent_Identity=26.8199233716475, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=52.8925619834711, Blast_Score=256, Evalue=9e-70,
Organism=Escherichia coli, GI1788935, Length=191, Percent_Identity=33.5078534031414, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790083, Length=151, Percent_Identity=33.7748344370861, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26279; Mature: 26148

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLKVLYGFHAVTARIRHDASTVEDVYYDATRKDRRMTEFLHAAKEAGVRLIAADETRL
CCEEEEEHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARAGDMPLAQNLAELLDGINGSPLILVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVVGTAGEAT
CCEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
KSLYDTELDGPVALVMGAEGEGMRRLTRDTCDVVMQIPMAGTVESLNVSVASGVCLFEAV
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCHHHCEEHHHHHHHHHHHH
RQRSVKK
HHHCCCC
>Mature Secondary Structure 
ARLKVLYGFHAVTARIRHDASTVEDVYYDATRKDRRMTEFLHAAKEAGVRLIAADETRL
CEEEEEHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARAGDMPLAQNLAELLDGINGSPLILVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVVGTAGEAT
CCEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCHHH
KSLYDTELDGPVALVMGAEGEGMRRLTRDTCDVVMQIPMAGTVESLNVSVASGVCLFEAV
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCHHHCEEHHHHHHHHHHHH
RQRSVKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]