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The map label for this gene is cspA [H]

Identifier: 91783230

GI number: 91783230

Start: 2071727

End: 2071930

Strand: Direct

Name: cspA [H]

Synonym: Bxe_A2596

Alternate gene names: 91783230

Gene position: 2071727-2071930 (Clockwise)

Preceding gene: 91783229

Following gene: 91783231

Centisome position: 42.32

GC content: 53.92

Gene sequence:

>204_bases
ATGGATACCGGTACTGTCAAATGGTTCAACGATAGCAAAGGCTTTGGCTTCATTACTCCTGATGCTGGCGGCGACGATCT
CTTCGCTCACTTCTCGGAAATTCGCGGCGACGGTTTCAAGACGCTCGCAGAAAACCAGAAGGTGAGCTACGAGACAAAGC
GCGGCCCGAAGGGTATGCAGGCCGCGAATATCTCGCCGCAGTAA

Upstream 100 bases:

>100_bases
ATTTTCCGCCTCTTCTGCGCGGTACTCGTTATCCTCTCCTCTTCCTTGAAGTTTTTGTCGCTCCGCAACGGAGCTGTTAT
TTCTATCTCAGGGAATTCTC

Downstream 100 bases:

>100_bases
AGTATTTCAAGGGCCGGTCTACCGGCCCTTTGGTTATTCGCCTGACTACCATCCTCGCGGAGCCATGCGCTCCCGATCTG
ATCGCCGGGTTTCAACGTGC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
MDTGTVKWFNDSKGFGFITPDAGGDDLFAHFSEIRGDGFKTLAENQKVSYETKRGPKGMQAANISPQ

Sequences:

>Translated_67_residues
MDTGTVKWFNDSKGFGFITPDAGGDDLFAHFSEIRGDGFKTLAENQKVSYETKRGPKGMQAANISPQ
>Mature_67_residues
MDTGTVKWFNDSKGFGFITPDAGGDDLFAHFSEIRGDGFKTLAENQKVSYETKRGPKGMQAANISPQ

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=54.5454545454545, Blast_Score=86, Evalue=3e-19,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=62.2950819672131, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=61.2903225806452, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=58.7301587301587, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=56.4516129032258, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=56.4516129032258, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=54.8387096774194, Blast_Score=74, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=43.5483870967742, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7314; Mature: 7314

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGTVKWFNDSKGFGFITPDAGGDDLFAHFSEIRGDGFKTLAENQKVSYETKRGPKGMQ
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCC
AANISPQ
CCCCCCC
>Mature Secondary Structure
MDTGTVKWFNDSKGFGFITPDAGGDDLFAHFSEIRGDGFKTLAENQKVSYETKRGPKGMQ
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCC
AANISPQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]