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The map label for this gene is idnO [C]

Identifier: 91783110

GI number: 91783110

Start: 1960766

End: 1961539

Strand: Direct

Name: idnO [C]

Synonym: Bxe_A2697

Alternate gene names: 91783110

Gene position: 1960766-1961539 (Clockwise)

Preceding gene: 91783109

Following gene: 91783111

Centisome position: 40.05

GC content: 62.79

Gene sequence:

>774_bases
ATGTCACGGATCTTCATTACTGGTTCGACCGATGGCCTGGGATTCATGGCCGCCGAATTACTTCTTTCCCAGGGACACGA
AGTCGTGCTGCACGCGCGCAGTCCGCGGCGTGCCGACAACGTGCGCCAGCGTCTTCCCGGCGCTGCCGCGATTGTGACCG
GCGACCTCTCGCGCATAGCGGAAATGAAATCGGCTGCGGAGCAGGTCAACCGGCTCGGAACGTTCGACGCAGTCATTCAT
AACGTGGGTATCGGCTACCGCGAGCCGAAAAGAATCGAGACGGAAGACGGACTGCCACATGTCTTTGCCGTCAACGTGAT
GGCGCCGTACGTGCTGACGGCTCTGATCGAGCGACCACGTCGTCTCGTGTACCTCAGTTCCGGCATGCACCATGGGGTTA
CCGCGAACCTCGGGGACACACGCTGGAAGCAGCGCCGCTGGAATGGCTCCGAGGCCTACGCCGAGAGCAAGCTGTACGAC
GTTCTGCTCGCCTTTGCCATTGCCCGCCGCTGGCCCGACGTCATGTCCAATGCGCTGGAGCCCGGCTGGGTGCCGACGCG
CATGGGCGGCCCTTCGGCACCGGACGATCTCGATCAGGCACATCGCACGCAGGTCTGGCTGGCCGCCAGTGATGATCCGG
GTGCGATGGTCTCGGGCGAGTATTTTTACCACCTGCAGCGGCGTGCGCCGAATCCCGCAACGCGAGATCACAGCACGCAG
GACAGGCTGATTGATCTGTGCGAGTCGTTCTCCGGTATCCGGCTGCCCGATTGA

Upstream 100 bases:

>100_bases
ACGCCGCGTGGGTCGGGATCGCAGGCATGCTGGCCGGCGGCCTCGCACTGGTCCTCGCGAAGTCATTGAGGTCTTCGTAA
TCGTACTGCCGGATATTGCT

Downstream 100 bases:

>100_bases
GCACGCGGCCAAAGATGCGTCCCGACTGTTTCACAAATGGAAACCCTATGAACATCAAAGGTTGTACGGCACTCGTCACA
GGTGCCAATCGAGGCTTGGG

Product: putative daunorubicin C-13 ketoreductase

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSRIFITGSTDGLGFMAAELLLSQGHEVVLHARSPRRADNVRQRLPGAAAIVTGDLSRIAEMKSAAEQVNRLGTFDAVIH
NVGIGYREPKRIETEDGLPHVFAVNVMAPYVLTALIERPRRLVYLSSGMHHGVTANLGDTRWKQRRWNGSEAYAESKLYD
VLLAFAIARRWPDVMSNALEPGWVPTRMGGPSAPDDLDQAHRTQVWLAASDDPGAMVSGEYFYHLQRRAPNPATRDHSTQ
DRLIDLCESFSGIRLPD

Sequences:

>Translated_257_residues
MSRIFITGSTDGLGFMAAELLLSQGHEVVLHARSPRRADNVRQRLPGAAAIVTGDLSRIAEMKSAAEQVNRLGTFDAVIH
NVGIGYREPKRIETEDGLPHVFAVNVMAPYVLTALIERPRRLVYLSSGMHHGVTANLGDTRWKQRRWNGSEAYAESKLYD
VLLAFAIARRWPDVMSNALEPGWVPTRMGGPSAPDDLDQAHRTQVWLAASDDPGAMVSGEYFYHLQRRAPNPATRDHSTQ
DRLIDLCESFSGIRLPD
>Mature_256_residues
SRIFITGSTDGLGFMAAELLLSQGHEVVLHARSPRRADNVRQRLPGAAAIVTGDLSRIAEMKSAAEQVNRLGTFDAVIHN
VGIGYREPKRIETEDGLPHVFAVNVMAPYVLTALIERPRRLVYLSSGMHHGVTANLGDTRWKQRRWNGSEAYAESKLYDV
LLAFAIARRWPDVMSNALEPGWVPTRMGGPSAPDDLDQAHRTQVWLAASDDPGAMVSGEYFYHLQRRAPNPATRDHSTQD
RLIDLCESFSGIRLPD

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=289, Percent_Identity=27.681660899654, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI10190746, Length=293, Percent_Identity=28.3276450511945, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI19923931, Length=232, Percent_Identity=29.3103448275862, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535059, Length=178, Percent_Identity=30.8988764044944, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 28526; Mature: 28394

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFITGSTDGLGFMAAELLLSQGHEVVLHARSPRRADNVRQRLPGAAAIVTGDLSRIA
CCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHH
EMKSAAEQVNRLGTFDAVIHNVGIGYREPKRIETEDGLPHVFAVNVMAPYVLTALIERPR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
RLVYLSSGMHHGVTANLGDTRWKQRRWNGSEAYAESKLYDVLLAFAIARRWPDVMSNALE
EEEEEECCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC
PGWVPTRMGGPSAPDDLDQAHRTQVWLAASDDPGAMVSGEYFYHLQRRAPNPATRDHSTQ
CCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCEECCHHHHHHHHCCCCCCCCCCCHH
DRLIDLCESFSGIRLPD
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRIFITGSTDGLGFMAAELLLSQGHEVVLHARSPRRADNVRQRLPGAAAIVTGDLSRIA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHHHH
EMKSAAEQVNRLGTFDAVIHNVGIGYREPKRIETEDGLPHVFAVNVMAPYVLTALIERPR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
RLVYLSSGMHHGVTANLGDTRWKQRRWNGSEAYAESKLYDVLLAFAIARRWPDVMSNALE
EEEEEECCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC
PGWVPTRMGGPSAPDDLDQAHRTQVWLAASDDPGAMVSGEYFYHLQRRAPNPATRDHSTQ
CCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCEECCHHHHHHHHCCCCCCCCCCCHH
DRLIDLCESFSGIRLPD
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} 20 {D-gluconate}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]