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The map label for this gene is paaF [C]

Identifier: 91783061

GI number: 91783061

Start: 1906074

End: 1906862

Strand: Direct

Name: paaF [C]

Synonym: Bxe_A2749

Alternate gene names: 91783061

Gene position: 1906074-1906862 (Clockwise)

Preceding gene: 91783052

Following gene: 91783063

Centisome position: 38.93

GC content: 65.78

Gene sequence:

>789_bases
ATGAGCGAGCCGTCCGTTGTCGCAGTGAGCCGCGCCGGCACCGTCGGCGTCATCGAGCTCGCACGCCCTGAAAAATTCAA
TTGCCTGTCGCTTGCGGTGTTCGCGGCGATCAGCGCGGCTGTGGACGCGTTCGAGACGCCTGAGTCGGGCGTGCGCTCGA
TCATGATCTGCGCTCAAGGCAAGAACTTCTGCACCGGCGCGGATCTGGACGAAGTGCTTTCGCTGCGCCAGGAGATCGGC
GATATGCGCCGTTTTATCAGCACTGCGCACCAAACCATGAAGCGGCTGTCCACGAGCAGCCTGCCGGTTGTTGCGGCGTG
CCAGGGTTTATCGCTAGCCGGCGGCTTCGAATTGATGCTGGCCTGCGACATTGCGATCGCCGCACGAGATGCGCGCTTCG
GCGATCAGCACGCACAGTACGGCCTGCTGCCGGGCTTCGGCGCGAGCCAGCGGATTCCCCGTTTGATCGGGTTGCGCCGC
AGCATGGATCTGTTCTTCTCGGCGCGCTGGCTCGATGCGCAGACCGCGCAGCAATGGGGTCTCGTGAATCGCGTCGTCGA
AGCGGGCGAACTGAGGCAGGCGGCGCTCGACTACTGCGAGGAGCTGGCCACGCGCAGCCGCATCGGGCTTGCAACGATGA
AGCGGCTGGCGCGCGAGGGACTGGAAGGGTCGCTGGAGGCGGGACTGAAGCTGGAGGAGGCGGTCGTGCCGGGCGGCCTG
CTGGAAGACGACGTGAGCGAAGGACTGGCGGCGTTCCAGGAGCGACGCTCGCCCCGGTTCCGCTCATAG

Upstream 100 bases:

>100_bases
AGTCAGTCCAATTGACACGTGGTATTCATGCGGCTATCGTGGGCGGCGAATCCGGGAGGATTGGCGGCCCGGAAAGTGTC
GGACAGAGGGAGAACCCAGC

Downstream 100 bases:

>100_bases
GTTGCGGGCCGCACCCGCGCGGCCTTTCCGATCAGACTGCCGAGATACCGCCCGTGATGCTGATCGCCTGACCGGTGATC
TTGGCGGCGGCCGGCGACGC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSEPSVVAVSRAGTVGVIELARPEKFNCLSLAVFAAISAAVDAFETPESGVRSIMICAQGKNFCTGADLDEVLSLRQEIG
DMRRFISTAHQTMKRLSTSSLPVVAACQGLSLAGGFELMLACDIAIAARDARFGDQHAQYGLLPGFGASQRIPRLIGLRR
SMDLFFSARWLDAQTAQQWGLVNRVVEAGELRQAALDYCEELATRSRIGLATMKRLAREGLEGSLEAGLKLEEAVVPGGL
LEDDVSEGLAAFQERRSPRFRS

Sequences:

>Translated_262_residues
MSEPSVVAVSRAGTVGVIELARPEKFNCLSLAVFAAISAAVDAFETPESGVRSIMICAQGKNFCTGADLDEVLSLRQEIG
DMRRFISTAHQTMKRLSTSSLPVVAACQGLSLAGGFELMLACDIAIAARDARFGDQHAQYGLLPGFGASQRIPRLIGLRR
SMDLFFSARWLDAQTAQQWGLVNRVVEAGELRQAALDYCEELATRSRIGLATMKRLAREGLEGSLEAGLKLEEAVVPGGL
LEDDVSEGLAAFQERRSPRFRS
>Mature_261_residues
SEPSVVAVSRAGTVGVIELARPEKFNCLSLAVFAAISAAVDAFETPESGVRSIMICAQGKNFCTGADLDEVLSLRQEIGD
MRRFISTAHQTMKRLSTSSLPVVAACQGLSLAGGFELMLACDIAIAARDARFGDQHAQYGLLPGFGASQRIPRLIGLRRS
MDLFFSARWLDAQTAQQWGLVNRVVEAGELRQAALDYCEELATRSRIGLATMKRLAREGLEGSLEAGLKLEEAVVPGGLL
EDDVSEGLAAFQERRSPRFRS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=30.6772908366534, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=35.4581673306773, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=27.3076923076923, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=210, Percent_Identity=27.6190476190476, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI68989263, Length=144, Percent_Identity=32.6388888888889, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI70995211, Length=210, Percent_Identity=30.4761904761905, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI213417737, Length=176, Percent_Identity=25.5681818181818, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=176, Percent_Identity=25.5681818181818, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI150378541, Length=137, Percent_Identity=32.1167883211679, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=31.2741312741313, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=29.0322580645161, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=28.968253968254, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=27.2373540856031, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=31.3513513513514, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=31.1881188118812, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=25.1937984496124, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=31.9230769230769, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=264, Percent_Identity=29.1666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=28.6363636363636, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=215, Percent_Identity=30.2325581395349, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=27.3062730627306, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=30.5220883534137, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=255, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=255, Percent_Identity=30.1960784313725, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=255, Percent_Identity=30.1960784313725, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=255, Percent_Identity=30.1960784313725, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=215, Percent_Identity=27.906976744186, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=28.1818181818182, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=29.3172690763052, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=29.3172690763052, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=30.5555555555556, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=28.125, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357171, Length=204, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24654903, Length=233, Percent_Identity=25.7510729613734, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28281; Mature: 28150

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPSVVAVSRAGTVGVIELARPEKFNCLSLAVFAAISAAVDAFETPESGVRSIMICAQG
CCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
KNFCTGADLDEVLSLRQEIGDMRRFISTAHQTMKRLSTSSLPVVAACQGLSLAGGFELML
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHH
ACDIAIAARDARFGDQHAQYGLLPGFGASQRIPRLIGLRRSMDLFFSARWLDAQTAQQWG
HHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LVNRVVEAGELRQAALDYCEELATRSRIGLATMKRLAREGLEGSLEAGLKLEEAVVPGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCCC
LEDDVSEGLAAFQERRSPRFRS
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEPSVVAVSRAGTVGVIELARPEKFNCLSLAVFAAISAAVDAFETPESGVRSIMICAQG
CCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
KNFCTGADLDEVLSLRQEIGDMRRFISTAHQTMKRLSTSSLPVVAACQGLSLAGGFELML
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHH
ACDIAIAARDARFGDQHAQYGLLPGFGASQRIPRLIGLRRSMDLFFSARWLDAQTAQQWG
HHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
LVNRVVEAGELRQAALDYCEELATRSRIGLATMKRLAREGLEGSLEAGLKLEEAVVPGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHCCCCC
LEDDVSEGLAAFQERRSPRFRS
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA