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The map label for this gene is truB [H]

Identifier: 91782991

GI number: 91782991

Start: 1826010

End: 1826945

Strand: Direct

Name: truB [H]

Synonym: Bxe_A2818

Alternate gene names: 91782991

Gene position: 1826010-1826945 (Clockwise)

Preceding gene: 91782990

Following gene: 91782996

Centisome position: 37.3

GC content: 65.6

Gene sequence:

>936_bases
ATGACCGCATCTCAACGCCCCCGCGTGCCGCGTCGCGCGCTCGACGGCGTGCTGCTGCTCGACAAGCCGCTCGGCCTGTC
GAGTAACGACGCGCTGATTCGCGCGAAGCGTCTCTATCTGGCAAAAAAAGCGGGCCACACCGGTACGCTCGATCCGTTGG
CCACCGGTTTGCTGCCGCTTTGCTTCGGCGAAGCCACCAAGTTTTCGCAGGACCTGCTCGAAGCCGACAAGACGTATGAA
GCCACCATGCGCCTCGGGATTCGCACGACCACCGGCGACGCCGAAGGCGAAGCAATCGACACGCGTGAAGTGACGTGCGA
CGAGGCGGCGATTCACGCGGTGCTGCCGGCATTCATCGGCGAAATCACCCAGGTTCCGCCGATGTATTCGGCGCTCAAGC
GTGACGGCAAGCCGCTGTACGAGTACGCGCGCGCGGGCCAGACCGTGGAGCGGGAAGGGCGCCAGGTGACGATCCTGAAG
CTCGAGATACTCGCTTGCGCGTTGCCGGACGTGACGTTTCGCGTGACATGCAGCAAGGGGACGTATGTGCGCACGCTGGC
CGAAGATATCGGCGAGGCGTTGGGTTGCGGCGCGCATCTGGTGGCGCTGCGGCGCACGGGCGTCGGCGCGCTGACGCTTG
AAAATTCGGTGACACTCGACGCGTTGTCCGACGCCGCCGAAAGCGAGCGCGACGCCTGGCTGCAGCCGGTGGATGCATTG
CTGTCCACCTTTCCGCTCGTGCAACTGAACGAAGACGCGACGCGCCGCTTTCTGCACGGTCAGCGGTTGAAACTGTCCGA
GTTGACCATCACGGGCGACGCGGTGAATGCGCCGCGTGTTCGGGTGTACGCCGCGCAAGGGCGCCTGCTTGGCGTCGCGA
AACAGGGCGAGGGCGTGCTGGCGCCCGAGCGGCTGGTCGTCACCACAGCGGGCTAA

Upstream 100 bases:

>100_bases
AGACTGAGCACGCGCTTAGCCGCGGCGCTCGCTGCCTTGCCTGAGTCGCATTGCGCTCAGAGCTGTTTTCCGAATTCATC
GAAGACTTCGTCTTTCCGTC

Downstream 100 bases:

>100_bases
ATACCGCACAGTCGGTAGACCGTGCGCGCAACGCGCACGGCCGATCGAAGGCAAAGCAAAAAAGGCGGGACCGCTATCGA
GCGGTCCCGCCTTTTTGCAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTASQRPRVPRRALDGVLLLDKPLGLSSNDALIRAKRLYLAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADKTYE
ATMRLGIRTTTGDAEGEAIDTREVTCDEAAIHAVLPAFIGEITQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTILK
LEILACALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLENSVTLDALSDAAESERDAWLQPVDAL
LSTFPLVQLNEDATRRFLHGQRLKLSELTITGDAVNAPRVRVYAAQGRLLGVAKQGEGVLAPERLVVTTAG

Sequences:

>Translated_311_residues
MTASQRPRVPRRALDGVLLLDKPLGLSSNDALIRAKRLYLAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADKTYE
ATMRLGIRTTTGDAEGEAIDTREVTCDEAAIHAVLPAFIGEITQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTILK
LEILACALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLENSVTLDALSDAAESERDAWLQPVDAL
LSTFPLVQLNEDATRRFLHGQRLKLSELTITGDAVNAPRVRVYAAQGRLLGVAKQGEGVLAPERLVVTTAG
>Mature_310_residues
TASQRPRVPRRALDGVLLLDKPLGLSSNDALIRAKRLYLAKKAGHTGTLDPLATGLLPLCFGEATKFSQDLLEADKTYEA
TMRLGIRTTTGDAEGEAIDTREVTCDEAAIHAVLPAFIGEITQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTILKL
EILACALPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLENSVTLDALSDAAESERDAWLQPVDALL
STFPLVQLNEDATRRFLHGQRLKLSELTITGDAVNAPRVRVYAAQGRLLGVAKQGEGVLAPERLVVTTAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=223, Percent_Identity=35.4260089686099, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI215599015, Length=304, Percent_Identity=28.6184210526316, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4503337, Length=304, Percent_Identity=28.6184210526316, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=45.5414012738853, Blast_Score=257, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17553978, Length=259, Percent_Identity=27.7992277992278, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=33.6734693877551, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=261, Percent_Identity=29.8850574712644, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364189, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364187, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364185, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364183, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471759, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17975520, Length=299, Percent_Identity=26.7558528428094, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33460; Mature: 33329

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASQRPRVPRRALDGVLLLDKPLGLSSNDALIRAKRLYLAKKAGHTGTLDPLATGLLPL
CCCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
CFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIDTREVTCDEAAIHAVLPAFIG
HHCCHHHHHHHHHHHCCHHEEHHEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
EITQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTILKLEILACALPDVTFRVTCSKG
HHHCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEEHHCCCCCEEEEEECCC
TYVRTLAEDIGEALGCGAHLVALRRTGVGALTLENSVTLDALSDAAESERDAWLQPVDAL
CHHHHHHHHHHHHHCCCHHEEEHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHH
LSTFPLVQLNEDATRRFLHGQRLKLSELTITGDAVNAPRVRVYAAQGRLLGVAKQGEGVL
HHHCCCEEECHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEECCCEEEEEECCCCCC
APERLVVTTAG
CCCEEEEEECC
>Mature Secondary Structure 
TASQRPRVPRRALDGVLLLDKPLGLSSNDALIRAKRLYLAKKAGHTGTLDPLATGLLPL
CCCCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
CFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIDTREVTCDEAAIHAVLPAFIG
HHCCHHHHHHHHHHHCCHHEEHHEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHH
EITQVPPMYSALKRDGKPLYEYARAGQTVEREGRQVTILKLEILACALPDVTFRVTCSKG
HHHCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEEHHCCCCCEEEEEECCC
TYVRTLAEDIGEALGCGAHLVALRRTGVGALTLENSVTLDALSDAAESERDAWLQPVDAL
CHHHHHHHHHHHHHCCCHHEEEHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHH
LSTFPLVQLNEDATRRFLHGQRLKLSELTITGDAVNAPRVRVYAAQGRLLGVAKQGEGVL
HHHCCCEEECHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEECCCEEEEEECCCCCC
APERLVVTTAG
CCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA