Click here to switch to the map view.

The map label for this gene is tcmA [H]

Identifier: 91782923

GI number: 91782923

Start: 1727545

End: 1729122

Strand: Direct

Name: tcmA [H]

Synonym: Bxe_A2897

Alternate gene names: 91782923

Gene position: 1727545-1729122 (Clockwise)

Preceding gene: 91782920

Following gene: 91782924

Centisome position: 35.29

GC content: 62.23

Gene sequence:

>1578_bases
ATGAATACTGCAACGTCATCCGGGACGGATAGCAACAGGCTGCTGATTCTTGCAGCCGTCTGTCTTTCCGCGCTAGTTTT
GCCTCTCGCCTTTTCAGGAGGCGCCGTCGCTACGCCCGCAATCGGGCGAGACCTGGGGGGTAGCCCCGCCATGCTCACCT
GGGTGACTAACGCTTTCATGCTGAGCTTCGGCAGCCTGCTAATGGCCGCCGGTGCGCTGGCTGACCAGTTCGGTCGCAAA
CGGCTCTTCGTCCTCGGTCTGGGCGGCTTCATCATCACTTCGTTCGCTATGAGCTTCGCGCCTTCGGTGCTGTGGCTCGA
TATTCTGCGCGGTGTTCAGGGTGTCGCGGCAGCGGCGTCGCTCTCCAGCGGCGGCGCATCGCTTGCGCAGGAGTTCGACG
GGCATGCACGTACGCGCGCATTCAGTATGCTCGGCACGAGCTTTGGCATCGGCCTGGCATTCGGACCGCTGATGGCCGGC
ATGCTGATCGAGCAATTTGGCTGGCGCTCGATTTTCCTCGTCATCGCTGCGATTGGCGTTGTCGCCTTCATCTTTGGCGT
GCCGCGTATGCGTGAGACGCGCGACCCGAACGCAACGGGGCTCGACTATCCGGGTACGGTCACTTTCACGGCGACGCTCG
CACTCTTCACGTTCGGCGTGATTCAGGCACCGGAAAGCGGATGGGGTAGCCCGCTTGTTGTGACGCTTCTCGTTGCATCG
GTTGTCTTTCTCGCCGCGTTCATCGTGGTCGAGACGCACGTCAAGCGTCCGATGCTCGAGTTGAGTCTATTCAGATATCC
GCGCTTTGTCGGCGTGCAGATTCTGCCGGTGGGCACCTGCTATTGCTACATCGTGCTGATCGTCATGTTGCCGTTGCGGT
TCATTGGTGCGGAAGGGTTGAGCGAAATCGACGCGGGCATGCTGATGATCGCGCTCTCGGCCCCGATGCTGGCCGTGCCG
ATGCTGGTTGCTTCGTTCACGCGCTGGATGTCCGCCGGCGTGCTGTCGGGCATCGGCTTTCTGATTGCCGCCGCGGGCCT
GTACTGGTTGAGTCTGGTCGACTTTAGCGGACCGAAAGCAGAGCTCATCGTGCCGATGCTGGTGATTGGTATCGGTGCGG
GTATGCCGTGGGGCCTGATGGATGGTCTGTCGGTCAGCGTGGTGCCGAAGGAGCGGGCCGGGATGGCCTCGGGCATCTTC
AGCACGACCCGTGTTGCGGGCGAAGGTATTGCTCTCGCAATCGCGACCGCAGTGCTCGGGGCGCTCGCGCACACGAGCCT
GTCGCGCACCATTCCTGACAATGGTGGTGACATCGCCGCTCGTATCGCAGAGGCCGCACAACGTGTGACCGCCGGTGACA
TGGTGCATGCCGCTTCGACCCTGCCGGAAGTCACCCGGCACGCGTTGTCGTCGAGCTACGCGGACGCGTTCACGAGCCTC
CTGCACGTGCTGATTGTCATCACGTTGCTGTCGGCGTTCGCCACCTTCGCGTTCCTGAGCAGGACCCCGGCGCAGGATGA
CGAACCGGGTGCGAAGTCTGACCGGAAGGATGCTGCTGAAGAGGCTGTTAGCGCATAG

Upstream 100 bases:

>100_bases
TCTTACGCAATTCGCTGTTCAGAATGACGTCCTTCGAAGGCCTCATTCCGCGTGAGCCCGTCGACGTGCTTCGCACTCCA
ATCACTTCACAGGTTCACCG

Downstream 100 bases:

>100_bases
CGCTCGTGTGACGGCGCTAAGGGGGCTGTCCCGCAACGGGGTAGCCTCCTTTCGCGTCAGTCGGGTCAATCGGGCTCCTA
CAGGGCCGCAGGCGCCTGAT

Product: major facilitator superfamily drug efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNTATSSGTDSNRLLILAAVCLSALVLPLAFSGGAVATPAIGRDLGGSPAMLTWVTNAFMLSFGSLLMAAGALADQFGRK
RLFVLGLGGFIITSFAMSFAPSVLWLDILRGVQGVAAAASLSSGGASLAQEFDGHARTRAFSMLGTSFGIGLAFGPLMAG
MLIEQFGWRSIFLVIAAIGVVAFIFGVPRMRETRDPNATGLDYPGTVTFTATLALFTFGVIQAPESGWGSPLVVTLLVAS
VVFLAAFIVVETHVKRPMLELSLFRYPRFVGVQILPVGTCYCYIVLIVMLPLRFIGAEGLSEIDAGMLMIALSAPMLAVP
MLVASFTRWMSAGVLSGIGFLIAAAGLYWLSLVDFSGPKAELIVPMLVIGIGAGMPWGLMDGLSVSVVPKERAGMASGIF
STTRVAGEGIALAIATAVLGALAHTSLSRTIPDNGGDIAARIAEAAQRVTAGDMVHAASTLPEVTRHALSSSYADAFTSL
LHVLIVITLLSAFATFAFLSRTPAQDDEPGAKSDRKDAAEEAVSA

Sequences:

>Translated_525_residues
MNTATSSGTDSNRLLILAAVCLSALVLPLAFSGGAVATPAIGRDLGGSPAMLTWVTNAFMLSFGSLLMAAGALADQFGRK
RLFVLGLGGFIITSFAMSFAPSVLWLDILRGVQGVAAAASLSSGGASLAQEFDGHARTRAFSMLGTSFGIGLAFGPLMAG
MLIEQFGWRSIFLVIAAIGVVAFIFGVPRMRETRDPNATGLDYPGTVTFTATLALFTFGVIQAPESGWGSPLVVTLLVAS
VVFLAAFIVVETHVKRPMLELSLFRYPRFVGVQILPVGTCYCYIVLIVMLPLRFIGAEGLSEIDAGMLMIALSAPMLAVP
MLVASFTRWMSAGVLSGIGFLIAAAGLYWLSLVDFSGPKAELIVPMLVIGIGAGMPWGLMDGLSVSVVPKERAGMASGIF
STTRVAGEGIALAIATAVLGALAHTSLSRTIPDNGGDIAARIAEAAQRVTAGDMVHAASTLPEVTRHALSSSYADAFTSL
LHVLIVITLLSAFATFAFLSRTPAQDDEPGAKSDRKDAAEEAVSA
>Mature_525_residues
MNTATSSGTDSNRLLILAAVCLSALVLPLAFSGGAVATPAIGRDLGGSPAMLTWVTNAFMLSFGSLLMAAGALADQFGRK
RLFVLGLGGFIITSFAMSFAPSVLWLDILRGVQGVAAAASLSSGGASLAQEFDGHARTRAFSMLGTSFGIGLAFGPLMAG
MLIEQFGWRSIFLVIAAIGVVAFIFGVPRMRETRDPNATGLDYPGTVTFTATLALFTFGVIQAPESGWGSPLVVTLLVAS
VVFLAAFIVVETHVKRPMLELSLFRYPRFVGVQILPVGTCYCYIVLIVMLPLRFIGAEGLSEIDAGMLMIALSAPMLAVP
MLVASFTRWMSAGVLSGIGFLIAAAGLYWLSLVDFSGPKAELIVPMLVIGIGAGMPWGLMDGLSVSVVPKERAGMASGIF
STTRVAGEGIALAIATAVLGALAHTSLSRTIPDNGGDIAARIAEAAQRVTAGDMVHAASTLPEVTRHALSSSYADAFTSL
LHVLIVITLLSAFATFAFLSRTPAQDDEPGAKSDRKDAAEEAVSA

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=378, Percent_Identity=26.984126984127, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788710, Length=480, Percent_Identity=23.5416666666667, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790195, Length=397, Percent_Identity=23.9294710327456, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789042, Length=415, Percent_Identity=23.6144578313253, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790146, Length=187, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=237, Percent_Identity=28.6919831223629, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54552; Mature: 54552

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTATSSGTDSNRLLILAAVCLSALVLPLAFSGGAVATPAIGRDLGGSPAMLTWVTNAFM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LSFGSLLMAAGALADQFGRKRLFVLGLGGFIITSFAMSFAPSVLWLDILRGVQGVAAAAS
HHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGGASLAQEFDGHARTRAFSMLGTSFGIGLAFGPLMAGMLIEQFGWRSIFLVIAAIGV
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAFIFGVPRMRETRDPNATGLDYPGTVTFTATLALFTFGVIQAPESGWGSPLVVTLLVAS
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VVFLAAFIVVETHVKRPMLELSLFRYPRFVGVQILPVGTCYCYIVLIVMLPLRFIGAEGL
HHHHHHHHHHHHHHHCHHHHHHHHHCCHHHCEEEEEHHHHHHHHHHHHHHHHHHHCCCCH
SEIDAGMLMIALSAPMLAVPMLVASFTRWMSAGVLSGIGFLIAAAGLYWLSLVDFSGPKA
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ELIVPMLVIGIGAGMPWGLMDGLSVSVVPKERAGMASGIFSTTRVAGEGIALAIATAVLG
HHHHHHHHHHHCCCCCCHHHCCCEEEECCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ALAHTSLSRTIPDNGGDIAARIAEAAQRVTAGDMVHAASTLPEVTRHALSSSYADAFTSL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHVLIVITLLSAFATFAFLSRTPAQDDEPGAKSDRKDAAEEAVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNTATSSGTDSNRLLILAAVCLSALVLPLAFSGGAVATPAIGRDLGGSPAMLTWVTNAFM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LSFGSLLMAAGALADQFGRKRLFVLGLGGFIITSFAMSFAPSVLWLDILRGVQGVAAAAS
HHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGGASLAQEFDGHARTRAFSMLGTSFGIGLAFGPLMAGMLIEQFGWRSIFLVIAAIGV
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAFIFGVPRMRETRDPNATGLDYPGTVTFTATLALFTFGVIQAPESGWGSPLVVTLLVAS
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VVFLAAFIVVETHVKRPMLELSLFRYPRFVGVQILPVGTCYCYIVLIVMLPLRFIGAEGL
HHHHHHHHHHHHHHHCHHHHHHHHHCCHHHCEEEEEHHHHHHHHHHHHHHHHHHHCCCCH
SEIDAGMLMIALSAPMLAVPMLVASFTRWMSAGVLSGIGFLIAAAGLYWLSLVDFSGPKA
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ELIVPMLVIGIGAGMPWGLMDGLSVSVVPKERAGMASGIFSTTRVAGEGIALAIATAVLG
HHHHHHHHHHHCCCCCCHHHCCCEEEECCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ALAHTSLSRTIPDNGGDIAARIAEAAQRVTAGDMVHAASTLPEVTRHALSSSYADAFTSL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHVLIVITLLSAFATFAFLSRTPAQDDEPGAKSDRKDAAEEAVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]