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The map label for this gene is citA [H]

Identifier: 91782869

GI number: 91782869

Start: 1656598

End: 1657998

Strand: Direct

Name: citA [H]

Synonym: Bxe_A2954

Alternate gene names: 91782869

Gene position: 1656598-1657998 (Clockwise)

Preceding gene: 91782868

Following gene: 91782872

Centisome position: 33.84

GC content: 63.24

Gene sequence:

>1401_bases
ATGGACTATTCCGCCACACCGCGGCCTGCCGGCACTGAAGCGTTGGCCATGCAGAGCGCGTCGGCAGTGCAGCCCGCGAG
GCGCGCGAGCCACGCCAAAGCGATTGCCGCGATTACGCTGGGCAACGGCCTCGAGTTTTTCGACTTCACGATCTACAGTT
TCTTCGCGACGATCATCGGCAAACTGTATTTCCCGGTGGAAGGGCAACTCGCGCAACTGATGCTGGCGGTCGGCACGTTC
GGTGTTGGCTTCATCATGCGTCCGGTGGGCGGCATCGTGCTCGGTGCGTACGCCGATCGCGCGGGTCGCAAGGCCGCAAT
GAGCCTCACGCTGTGGCTGATGACGCTCGGCTCCGCGATCATCGCATTTGCGCCGACTTACGCGGCGATCGGCATTGCCG
CGCCGCTGCTGGTGATTCTGGCGCGGCTGATTCAGGGTTTTGCGCTGGGCGGCGAGATCGGCGCGTCGACGTCGCTGTTG
CTGGAATACGGCAGCAACAGCACGCGCGGTTTCTATGGCAGTTGGCAGTTCGTGAGCCAGGGCTTGAACACGGTGTGCGG
ATCGCTGCTGGGTGTCGCGCTGGCGGCGGCGCTTTCGACCGCGGCGCTGGAGAGTTGGGGCTGGCGCGTGCCGTTCGTGA
TCGGCATGGCAATGGGGCCGATCGGCATCTATATCCGGCGACATCTCGATGAGACGCTGCCGGGTGTCGAAGACGGCCCG
GCGCCGCAGCCGGGCGAGGGTGCGGCGGCGGCTTTACAGCCTGGCTCGCAGCCGGTCCCGCGGGCTGTCTCGCAGCCGGT
GCGCACGCTGTTTCGCGAGCATTCACGCGTCATCACGACGGGCGTTCTGACGACGATCGGCGGCACGGCGGCAAACTATA
TCGTGCTGTTTTATCTGTCCACCTACGCGATCAGGATCCTGCATCTGCCGATGTCGTCGGCACTCTGGGCCGCGTGGACT
GCCGCGATGGTGACGGTGATCTGTTCGCCTTTTGCCGGCGCGTTGTCGGATCGCGTTGGGCGCAAGCGGGTGTTGTGGGT
TTCTCGCGTGCTGCTGATCGTCGCCGTGTATCCGGCGTTCATGATGATCAATGCGTGGCCGACAGTGCCGGTTCTGCTGT
CGGTAGTTGCCGGCCTGGCCGTGCTGGTTGCGTTGACCGCCGTGCCGAATATCGTCATGTTGCCGGAGATGTTTCCCCGC
GAAATTCGCGCGACCGGCATGTCGATTGTTTATTGTCTCGGTGTTTCTATTTTCGGCGGATTCGCCCAGTTTTTTGCTAC
CTGGCTGATTCAGGTTTCAGGGAGTAACCTCGCGCCGGCGTGGTATCTGATCGGCTGCGGGCTGGTTTCCTTGTTGCCGT
TGCCGTTTATGCAAGAGACTGCCGGGCGGGATATCGGGTAG

Upstream 100 bases:

>100_bases
TAAGACTCGCGCAGACGTTTCTGGTCTGAGGCCGGGGCTTCTGCATAAGCGGGCAGCGCCGCGTATGCGCATCAGCAGCA
AGAACATCACGGAGACACAG

Downstream 100 bases:

>100_bases
GGTTTTTTTGCCCGCGCGGCGGTGTTCTTCCGGATGCCGCGCAGGCAAAAAAGCGGCAGTCACCGAGCCGCCGACTTCCC
TCCGTCCGCCACCGGCGCTT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDYSATPRPAGTEALAMQSASAVQPARRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTF
GVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAIIAFAPTYAAIGIAAPLLVILARLIQGFALGGEIGASTSLL
LEYGSNSTRGFYGSWQFVSQGLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLDETLPGVEDGP
APQPGEGAAAALQPGSQPVPRAVSQPVRTLFREHSRVITTGVLTTIGGTAANYIVLFYLSTYAIRILHLPMSSALWAAWT
AAMVTVICSPFAGALSDRVGRKRVLWVSRVLLIVAVYPAFMMINAWPTVPVLLSVVAGLAVLVALTAVPNIVMLPEMFPR
EIRATGMSIVYCLGVSIFGGFAQFFATWLIQVSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRDIG

Sequences:

>Translated_466_residues
MDYSATPRPAGTEALAMQSASAVQPARRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTF
GVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAIIAFAPTYAAIGIAAPLLVILARLIQGFALGGEIGASTSLL
LEYGSNSTRGFYGSWQFVSQGLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLDETLPGVEDGP
APQPGEGAAAALQPGSQPVPRAVSQPVRTLFREHSRVITTGVLTTIGGTAANYIVLFYLSTYAIRILHLPMSSALWAAWT
AAMVTVICSPFAGALSDRVGRKRVLWVSRVLLIVAVYPAFMMINAWPTVPVLLSVVAGLAVLVALTAVPNIVMLPEMFPR
EIRATGMSIVYCLGVSIFGGFAQFFATWLIQVSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRDIG
>Mature_466_residues
MDYSATPRPAGTEALAMQSASAVQPARRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTF
GVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAIIAFAPTYAAIGIAAPLLVILARLIQGFALGGEIGASTSLL
LEYGSNSTRGFYGSWQFVSQGLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLDETLPGVEDGP
APQPGEGAAAALQPGSQPVPRAVSQPVRTLFREHSRVITTGVLTTIGGTAANYIVLFYLSTYAIRILHLPMSSALWAAWT
AAMVTVICSPFAGALSDRVGRKRVLWVSRVLLIVAVYPAFMMINAWPTVPVLLSVVAGLAVLVALTAVPNIVMLPEMFPR
EIRATGMSIVYCLGVSIFGGFAQFFATWLIQVSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRDIG

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=438, Percent_Identity=32.1917808219178, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=31.1475409836066, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788292, Length=441, Percent_Identity=25.6235827664399, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1789941, Length=441, Percent_Identity=30.1587301587302, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87082231, Length=349, Percent_Identity=25.5014326647564, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49183; Mature: 49183

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSATPRPAGTEALAMQSASAVQPARRASHAKAIAAITLGNGLEFFDFTIYSFFATIIG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHH
KLYFPVEGQLAQLMLAVGTFGVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
IAFAPTYAAIGIAAPLLVILARLIQGFALGGEIGASTSLLLEYGSNSTRGFYGSWQFVSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCCCHHHHHH
GLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLDETLPGVEDGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
APQPGEGAAAALQPGSQPVPRAVSQPVRTLFREHSRVITTGVLTTIGGTAANYIVLFYLS
CCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TYAIRILHLPMSSALWAAWTAAMVTVICSPFAGALSDRVGRKRVLWVSRVLLIVAVYPAF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MMINAWPTVPVLLSVVAGLAVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIFGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
FAQFFATWLIQVSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRDIG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MDYSATPRPAGTEALAMQSASAVQPARRASHAKAIAAITLGNGLEFFDFTIYSFFATIIG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHH
KLYFPVEGQLAQLMLAVGTFGVGFIMRPVGGIVLGAYADRAGRKAAMSLTLWLMTLGSAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
IAFAPTYAAIGIAAPLLVILARLIQGFALGGEIGASTSLLLEYGSNSTRGFYGSWQFVSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCCCCCCHHHHHH
GLNTVCGSLLGVALAAALSTAALESWGWRVPFVIGMAMGPIGIYIRRHLDETLPGVEDGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
APQPGEGAAAALQPGSQPVPRAVSQPVRTLFREHSRVITTGVLTTIGGTAANYIVLFYLS
CCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TYAIRILHLPMSSALWAAWTAAMVTVICSPFAGALSDRVGRKRVLWVSRVLLIVAVYPAF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MMINAWPTVPVLLSVVAGLAVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIFGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
FAQFFATWLIQVSGSNLAPAWYLIGCGLVSLLPLPFMQETAGRDIG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]