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The map label for this gene is puuR [H]

Identifier: 91782830

GI number: 91782830

Start: 1605505

End: 1606080

Strand: Direct

Name: puuR [H]

Synonym: Bxe_A2995

Alternate gene names: 91782830

Gene position: 1605505-1606080 (Clockwise)

Preceding gene: 91782829

Following gene: 91782831

Centisome position: 32.79

GC content: 56.25

Gene sequence:

>576_bases
ATGGTTTTGCCACTCAATAACAAGGCACACGTTGCAGTCGCTATAGGAAGCAAAATCCGCGCGCTTCGCCAACGGCTCAA
GCGCACGCTGGATGAAGTGGCAAAGACTGCGGGCATTTCCAAGCCCTTTCTGTCGCAGGTGGAACGGGGTCACGCAACCC
CTTCGATTACATCGCTGGTTGGTATTGCCCGAGCTTTAGGTGTCACGGTGCAGTACTTCGTCGACACGCCGACCGAAGAC
AAGTCGGTGTTGAGAGGGAGTGATCTGAAGTACTTCGGTTTTGACGGAAGCGCCAATCTGTTTGGGCGGCTCACGAATCT
GTCGGTCGGCAGCAAGCTGGAGGTCATCCTCGTCAGAATGCCGGTGGGACAGAATCCGTCGGAGGTCACGACGCACGCAG
GCGAAGAGTTTCTGTATGTAATGAGCGGCCAGATCTCGCTCACGCTGGAAGGTACGACGTTCGTGTTGCAGGCGGGTGAC
ACCGCGCATTACGAGTCTACTGTGCCGCACTCCTGGTCCAATACCGCACGTGAAGAAGCGGTGATCGTGTGGGTGGGTAC
ACCAAGATTGTTCTGA

Upstream 100 bases:

>100_bases
CCGGCAGGCGTACCAGGGAGGCTTGAGGAGGGTTGCAGCGTTTGATGAAACTTAATTTGCTTATATCAAACTATTTTTGA
CTACGGGATTCTGTCTTGCT

Downstream 100 bases:

>100_bases
TGCCGTAGGCCATGCTTCTGAAAAAAGCAGGCATTCTGTATTTGTAAGCATTACTTAAGTCTATATCCGCGATTTGGGAA
AAACAGGCCGGCCAATGGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MVLPLNNKAHVAVAIGSKIRALRQRLKRTLDEVAKTAGISKPFLSQVERGHATPSITSLVGIARALGVTVQYFVDTPTED
KSVLRGSDLKYFGFDGSANLFGRLTNLSVGSKLEVILVRMPVGQNPSEVTTHAGEEFLYVMSGQISLTLEGTTFVLQAGD
TAHYESTVPHSWSNTAREEAVIVWVGTPRLF

Sequences:

>Translated_191_residues
MVLPLNNKAHVAVAIGSKIRALRQRLKRTLDEVAKTAGISKPFLSQVERGHATPSITSLVGIARALGVTVQYFVDTPTED
KSVLRGSDLKYFGFDGSANLFGRLTNLSVGSKLEVILVRMPVGQNPSEVTTHAGEEFLYVMSGQISLTLEGTTFVLQAGD
TAHYESTVPHSWSNTAREEAVIVWVGTPRLF
>Mature_191_residues
MVLPLNNKAHVAVAIGSKIRALRQRLKRTLDEVAKTAGISKPFLSQVERGHATPSITSLVGIARALGVTVQYFVDTPTED
KSVLRGSDLKYFGFDGSANLFGRLTNLSVGSKLEVILVRMPVGQNPSEVTTHAGEEFLYVMSGQISLTLEGTTFVLQAGD
TAHYESTVPHSWSNTAREEAVIVWVGTPRLF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=183, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20726; Mature: 20726

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPLNNKAHVAVAIGSKIRALRQRLKRTLDEVAKTAGISKPFLSQVERGHATPSITSLV
CEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHH
GIARALGVTVQYFVDTPTEDKSVLRGSDLKYFGFDGSANLFGRLTNLSVGSKLEVILVRM
HHHHHHCCEEEEEECCCCCCCHHHCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEEEEE
PVGQNPSEVTTHAGEEFLYVMSGQISLTLEGTTFVLQAGDTAHYESTVPHSWSNTAREEA
CCCCCHHHHHHCCCCEEEEEEECEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCE
VIVWVGTPRLF
EEEEECCCCCC
>Mature Secondary Structure
MVLPLNNKAHVAVAIGSKIRALRQRLKRTLDEVAKTAGISKPFLSQVERGHATPSITSLV
CEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHH
GIARALGVTVQYFVDTPTEDKSVLRGSDLKYFGFDGSANLFGRLTNLSVGSKLEVILVRM
HHHHHHCCEEEEEECCCCCCCHHHCCCCEEEEECCCCCCCEEEEECCCCCCCEEEEEEEE
PVGQNPSEVTTHAGEEFLYVMSGQISLTLEGTTFVLQAGDTAHYESTVPHSWSNTAREEA
CCCCCHHHHHHCCCCEEEEEEECEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCE
VIVWVGTPRLF
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]