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The map label for this gene is ilvI [H]

Identifier: 91782597

GI number: 91782597

Start: 1364608

End: 1366371

Strand: Direct

Name: ilvI [H]

Synonym: Bxe_A3231

Alternate gene names: 91782597

Gene position: 1364608-1366371 (Clockwise)

Preceding gene: 91782585

Following gene: 91782598

Centisome position: 27.87

GC content: 61.45

Gene sequence:

>1764_bases
ATGAATATGCCCAGCGCGGAATTCTCCACGTCGGATACGACCCCTCATCCCGAAGCTGACTCTATCGGCGCCACCGTGCT
CATGAAGGCACTGGCCGACGAAGACGTCGAGTTTGTCTGGGGCTATCCCGGCGGCTCGGTACTCTACATTTACGACGAGC
TGTACAAGCAGGACAAATTCCAGCACATCCTCGTGCGCCACGAGCAGGCCGCAGTCCACGCCGCCGACGCCTACGCGCGT
TCCACCGGCAAGGTCGGTGTGTGCCTCGTGACCTCCGGCCCCGGCGTCACCAATGCGGTGACCGGCATCGCCACTGCCTA
CATGGATTCGATTCCGATGGTGGTGATCAGCGGCCAGGTGCCGACTGCCGCGATCGGCCAGGATGCGTTCCAGGAGTGCG
ACACGGTCGGTATCACGCGTCCTTGCGTGAAGCACAACTTCCTCGTGAAGGACGTGCGCGACCTCGCCGCCACCGTCAAG
AAAGCCTTTTACATTGCCCGTACCGGCCGTCCCGGCCCCGTGCTGATCGACATTCCGAAAGACGTGTCGAAGGCGCCTTG
CCAGTACGAGCCGCTCAAAAGCGTGTCGCTGCGCTCGTACAACCCGGTCACGAAGGGCCACTCCGGCCAGATCCGCAAAG
CGGTCGCGCTGCTGTTGTCGGCGAAGCGTCCTTATATCTATACCGGCGGCGGCATCATTCTCGCGGACGCATCGCGTGAA
CTGAACCAGTTCGCCGATCTGCTTGGTTATCCCGTCACCAACACGTTGATGGGTCTGGGCGGCTACCGCGCGAGTGACAA
AAAATTCCTCGGCATGCTCGGCATGCACGGCACGTACGAAGCCAATATGGCGATGCAGCACTGCGACGTGCTGATCGCGA
TCGGCGCGCGTTTCGACGACCGTGTGATCGGCGACCCGGCGCACTTCGCGTCGCGTCCGCGCAAGATCATCCATATCGAC
ATCGACCCGTCCTCCATCTCCAAGCGCGTCAAGGTCGACATTCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGAGCT
GATCGAGCAGCTGCAAACGGCCGAGCACGGCCCGGACACCGCGGCGCTCGCCGACTGGTGGAAGGACATCGAGGCGTGGC
GCGCGAAAGACTGCCTCAAGTTCGACCGCAAGAGCGACATCATCAAGCCGCAGTACGTGGTGGAAAAGGCGTGGGAACTG
ACCGACGGCAATGCCTTCGTGTGTTCCGACGTCGGCCAGCATCAGATGTGGGCGGCGCAGTTCTATCGCTTCAACAAGCC
GCGCCGCTGGATCAACTCTGGTGGTCTCGGCACGATGGGCTTCGGCCTGCCGGCGGCGATGGGCGTGAAGATGGCGCACC
CGGACGACGACGTGCTCTGTATCACGGGCGAAGGCTCGATCCAGATGTGCATCCAGGAGCTCTCCACCTGCAAGCAGTAC
GAGACTCCGGTGAAGATCATTTCGCTGAACAACCGCTATCTGGGCATGGTGCGCCAGTGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCGGATTTCGTGAAGCTCGCCGAAGCGTACGGCCACGTGGGCATGCGTA
TCGAACGCACGGCGGATGTGGAGCCGGCGTTGAAGGAAGCGCTGCGCCTGAAAGATCGCACGGTGTTTCTCGATTTCCAG
ACCGATCCGACCGAAAACGTCTGGCCGATGGTTCAGGCCGGCAAGGGCATCACCGAGATGCTCATGGGTTCGGAAGATCT
ATAA

Upstream 100 bases:

>100_bases
GAAGCTCACCTGTCAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGCCG
AAAATTCGAAAGGTGAATAA

Downstream 100 bases:

>100_bases
CGACGGCGCGCTTGGGTTCGTTCACCTGAGCGCAGCGTTGGCTTCGTCATGCGCGCCTTCATGAAAGGCGGGAGCGGACG
AGGCGGCGCGAACCGTTCGC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSDTTPHPEADSIGATVLMKALADEDVEFVWGYPGGSVLYIYDELYKQDKFQHILVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDVSKAPCQYEPLKSVSLRSYNPVTKGHSGQIRKAVALLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRKSDIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
ETPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLMGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSDTTPHPEADSIGATVLMKALADEDVEFVWGYPGGSVLYIYDELYKQDKFQHILVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDVSKAPCQYEPLKSVSLRSYNPVTKGHSGQIRKAVALLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRKSDIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
ETPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLMGSEDL
>Mature_587_residues
MNMPSAEFSTSDTTPHPEADSIGATVLMKALADEDVEFVWGYPGGSVLYIYDELYKQDKFQHILVRHEQAAVHAADAYAR
STGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVK
KAFYIARTGRPGPVLIDIPKDVSKAPCQYEPLKSVSLRSYNPVTKGHSGQIRKAVALLLSAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTAALADWWKDIEAWRAKDCLKFDRKSDIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
ETPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLMGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=25.0428816466552, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI21361361, Length=569, Percent_Identity=27.4165202108963, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=57.1929824561403, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=41.6071428571429, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=34.2056074766355, Blast_Score=308, Evalue=9e-85,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=27.9491833030853, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.5487364620939, Blast_Score=145, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=26.2206148282098, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=26.2206148282098, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=516, Percent_Identity=27.3255813953488, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=42.512908777969, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.4210526315789, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=524, Percent_Identity=22.5190839694656, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.0176991150442, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64894; Mature: 64894

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSDTTPHPEADSIGATVLMKALADEDVEFVWGYPGGSVLYIYDELYKQDKF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHILVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSKAPCQYEPLKSVSLRSYNPVTKGHSGQIRKAVALLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
AALADWWKDIEAWRAKDCLKFDRKSDIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHH
ETPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLMGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSDTTPHPEADSIGATVLMKALADEDVEFVWGYPGGSVLYIYDELYKQDKF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHILVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSKAPCQYEPLKSVSLRSYNPVTKGHSGQIRKAVALLLSAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
AALADWWKDIEAWRAKDCLKFDRKSDIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHH
ETPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLMGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]