Click here to switch to the map view.

The map label for this gene is purT

Identifier: 91782528

GI number: 91782528

Start: 1290699

End: 1291913

Strand: Direct

Name: purT

Synonym: Bxe_A3301

Alternate gene names: 91782528

Gene position: 1290699-1291913 (Clockwise)

Preceding gene: 91782525

Following gene: 91782529

Centisome position: 26.36

GC content: 67.57

Gene sequence:

>1215_bases
ATGCAGATCGGCCAACGGATCGGCACGCCCCTTTCTGAATCCGCCACGCGTGTCATGCTGCTCGGCGCCGGCGAGCTCGG
CAAGGAAGTGATCATCGCGCTGCAGCGGCTGGGCGTCGAAGTGATCGCCGTCGACCGTTACCCGAATGCGCCTGGCCACC
AGGTGGCGCACCGCGCGCACGTGATCGACATGACCGATCGCGCCGCGCTGCGCGCGCTGGTGGAACAGGAGCGGCCGCAT
CTGATCGTCCCCGAGATCGAGGCGATCGCGACGGACGAACTCGCCGCCATCGAAAGCGCCGGTCTCGCCGAAGTGATCCC
GACCGCGCGCGCCACGCAGCTCACCATGAACCGCGAAGGCATCCGCCGTCTCGCCGCTGAGGAACTCGGCCTGCCGACCT
CGCCGTACGCGTTCGCCGATTCGCTCGACGAACTGCGCGCGGGCATCGCCAAAGTCGGCTATCCGTGCGTGGTGAAGCCG
GTCATGTCGTCATCGGGCAAGGGCCAGTCGGTGCTGCGCGGCGACGCCGACGTCGAACCCGCATGGCAATACGCGATGGC
CGGCGGCCGCGTGAATCACGGCCGTGTGATCGTCGAAGGCTTCATCGACTTCGAATACGAAATCACGCAATTGACTGTGC
GCGCGATCAACCCCGCGAGCGGCGAGGTCAGCACGTATTTCTGCGACCCGATCGGCCACGTGCAGGTGGCGGGCGACTAC
GTGGAATCGTGGCAGCCACAGCCCATGAGCCCGCTCGCGCTCGAACGTTCGCGTGAAGTGGCGCACAAGGTGACGGCCGC
GCTCGGCGGGCGCGGCCTGTTCGGCGTGGAACTTTTCGTGCGCGGCGATGACGTATGGTTTTCGGAAGTGAGTCCGCGTC
CGCACGACACTGGTCTTGTCACGCTGTGCTCGCAACGCTTTTCGGAATTCGAACTGCACGCGCGCGCGATCCTCGGTTTG
CCGGTGGATACGTCGCTGCGGGCGCCGGGTGCGTCGGCGGTGATTTATGGCGGGCTCGACGAGGCCGGCATTGCGTTCGA
AGGCGTGGCCGCGGCGTTGGCGGTGCCGAACGCGGACCTGCGCCTGTTCGGCAAGCCGGAGAGCTTCGTCAAGCGCCGCA
TGGGCGTGGCGCTCGCCACCGGCGAGACCACCGACGAAGCCCGCTCGCGCGCGAAGCAGGCTGCGGCGGCGGTGCGGCCG
GTGTCGGCGAAATAA

Upstream 100 bases:

>100_bases
GATCGGGATGCGCCCGCGAGCAAGGTGCGGCGAGGGGTCCGCATGTTAACATCGTGTTCTACCCAATCCCACCCTCATCC
GACTTTTATCTGGAGACCCC

Downstream 100 bases:

>100_bases
GTTGGCGAAATGAGCCGGCGAGATGCACGGGAAGCGCCGTGACGAACGGCCACGCGTTGCCTGTCCCGCGCGCCCGGCAT
AATCGCCGCGCGCAATCCGG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQIGQRIGTPLSESATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDRAALRALVEQERPH
LIVPEIEAIATDELAAIESAGLAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKP
VMSSSGKGQSVLRGDADVEPAWQYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAINPASGEVSTYFCDPIGHVQVAGDY
VESWQPQPMSPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLVTLCSQRFSEFELHARAILGL
PVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADLRLFGKPESFVKRRMGVALATGETTDEARSRAKQAAAAVRP
VSAK

Sequences:

>Translated_404_residues
MQIGQRIGTPLSESATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDRAALRALVEQERPH
LIVPEIEAIATDELAAIESAGLAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKP
VMSSSGKGQSVLRGDADVEPAWQYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAINPASGEVSTYFCDPIGHVQVAGDY
VESWQPQPMSPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLVTLCSQRFSEFELHARAILGL
PVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADLRLFGKPESFVKRRMGVALATGETTDEARSRAKQAAAAVRP
VSAK
>Mature_404_residues
MQIGQRIGTPLSESATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAHVIDMTDRAALRALVEQERPH
LIVPEIEAIATDELAAIESAGLAEVIPTARATQLTMNREGIRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKP
VMSSSGKGQSVLRGDADVEPAWQYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAINPASGEVSTYFCDPIGHVQVAGDY
VESWQPQPMSPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLVTLCSQRFSEFELHARAILGL
PVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADLRLFGKPESFVKRRMGVALATGETTDEARSRAKQAAAAVRP
VSAK

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=394, Percent_Identity=61.6751269035533, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=23.5135135135135, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_BURXL (Q142Q7)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_557734.1
- ProteinModelPortal:   Q142Q7
- SMR:   Q142Q7
- GeneID:   4002370
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A3301
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43156; Mature: 43156

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 85-85 BINDING 118-118 BINDING 159-159 BINDING 207-207 BINDING 277-277 BINDING 289-289 BINDING 296-296 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGQRIGTPLSESATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAH
CCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDRAALRALVEQERPHLIVPEIEAIATDELAAIESAGLAEVIPTARATQLTMNREG
EEECHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECHHH
IRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKPVMSSSGKGQSVLRGDADVEP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECCCCCCCH
AWQYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAINPASGEVSTYFCDPIGHVQVAGDY
HHHHHHCCCCCCCCEEEEEEEECCEEEEEEEEEEEECCCCCCEEEEEECCCCCEEEECHH
VESWQPQPMSPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCHHH
TLCSQRFSEFELHARAILGLPVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADL
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCE
RLFGKPESFVKRRMGVALATGETTDEARSRAKQAAAAVRPVSAK
EEECCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIGQRIGTPLSESATRVMLLGAGELGKEVIIALQRLGVEVIAVDRYPNAPGHQVAHRAH
CCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEE
VIDMTDRAALRALVEQERPHLIVPEIEAIATDELAAIESAGLAEVIPTARATQLTMNREG
EEECHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECHHH
IRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKPVMSSSGKGQSVLRGDADVEP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECCCCCCCH
AWQYAMAGGRVNHGRVIVEGFIDFEYEITQLTVRAINPASGEVSTYFCDPIGHVQVAGDY
HHHHHHCCCCCCCCEEEEEEEECCEEEEEEEEEEEECCCCCCEEEEEECCCCCEEEECHH
VESWQPQPMSPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCHHH
TLCSQRFSEFELHARAILGLPVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADL
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCE
RLFGKPESFVKRRMGVALATGETTDEARSRAKQAAAAVRPVSAK
EEECCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA