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The map label for this gene is otsA [H]

Identifier: 91782457

GI number: 91782457

Start: 1203561

End: 1204988

Strand: Direct

Name: otsA [H]

Synonym: Bxe_A3374

Alternate gene names: 91782457

Gene position: 1203561-1204988 (Clockwise)

Preceding gene: 91782456

Following gene: 91782462

Centisome position: 24.58

GC content: 63.45

Gene sequence:

>1428_bases
ATGAGCAGACTGATCGTGGTATCGAATCGTGTCGCGCCGACTCAGGAAGGCCGTCCCGCAGCGGGCGGACTGGCGATCGG
CGTGCTGGACGCGCTGAAGGAAACCGGCGGAGTCTGGTTCGGCTGGAGCGGCGAGACGGTGAGCGAGCCGAGCGCTCCGG
TGATCGAGAAACAGGGCAACGTGACGTACGCGACGGTGGGTCTCACCAAGCGCGATTACGACCAGTACTACCGCGGTTTT
TCCAACGCGACGCTGTGGCCGACGTTCCATTACCGCAACGACCTCTCGCGCTACGACCGTCAGGAATACGCGGGCTATCA
GCGCGTGAACGCGACGCTCGCCAAGCAGCTCAAGGAGCTGCTGAAACCCGACGACATCATCTGGGTTCACGACTATCACC
TGCTGCCGTTCGCGCGCTGCCTGCGCGAGCTGGGCGTGAAGAATCCCATCGGCTTCTTCCTGCATATTCCGTTTCCGGTG
CCGGAGGTGCTGCGCACCATTCCGCCGCACGAAGAACTGGTCAAGGCCATGTGCAGCTACGACGTGATCGGCTTCCAGAC
CGATGCGGATCGCCAGTCGTTCGTCGACTTCATCGAGCGTGGCCAGCACGGCACATCGAGCGAGGACGGCATGGTGCACG
CGTACAACCGCTTCCTGAAAGTGGGCGCGTATCCGATCGGAATCTATCCGGACGCGATTGCCAAGGCCGCCGAGCAGTTC
ACCGACCGCAAGCCGGTGCGCAGCCTGCGCGACGGTATGCGCGGGCGCAAGCTGATCATGAGCGTGGATCGTCTCGACTA
TTCGAAGGGGCTGGTGGAGCGCTTTCAGGCGTTCGAGCGACTGTTGCTGAACGCACCCGGTTGGCACGGACGCGTATCGC
TGGTGCAGATCGCGCCGCCGACGCGTGCGGACGTGCAGACATATCAGCGGATTCGGCAGACGCTGGAGGGCGAAGCGGGC
CGCATCAACGGCCGGTTCGCGCAGCTCGACTGGACGCCGATCCAGTACCTGAACCGCAAATACGAGCGCAATCTGCTGAT
GGCGCTATTCCGTCAATCGCAGGTCGGTTATGTGACGCCGCTGCGCGACGGTATGAATCTGGTCGCGAAGGAGTATGTCG
CCTCGCAGGATCCGGCCGATCCGGGCGTGCTGGTGCTGTCGCAATTCGCCGGCGCCGCCGAGCAGTTGCCGGGCGCGCTG
GTCGTCAATCCGTTTGATCTGTCGCAGATGGCCGAAGCGCTTGAACGCGCTCTGTCCATGCCGCTCGCCGAGCGTCAGGC
GCGGCATGCCGACATGATGGCGCCGATGCGAGAGAACAATCTGTCGGTGTGGCGCGATACGTTCCTTGCCGATCTGCGCA
ATGTCGCCACGGCCTCTTCGGTGACGGCCAAAGCGGTAAAGCTCGCTGACGTGACCGCATCGTCGTAG

Upstream 100 bases:

>100_bases
ATTTTTATTTCCAATATCTTAAAAAACGGCACGCGACTTGCATGCACCTTTTTGGCACATATCCGTATGCAACGAAACAA
CTGAACTAGGTGGAGCACCA

Downstream 100 bases:

>100_bases
GCGGATCAACGGCCGCGCGGCTTGCACGAGTGCACGCCGCGTGTGCTGGTCACGTCATCGGGTAACAGCACAGATGGGCG
AAAGACCGCAAGGGCGCGAA

Product: Alpha, alpha-trehalose-phosphate synthase(UDP- forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGETVSEPSAPVIEKQGNVTYATVGLTKRDYDQYYRGF
SNATLWPTFHYRNDLSRYDRQEYAGYQRVNATLAKQLKELLKPDDIIWVHDYHLLPFARCLRELGVKNPIGFFLHIPFPV
PEVLRTIPPHEELVKAMCSYDVIGFQTDADRQSFVDFIERGQHGTSSEDGMVHAYNRFLKVGAYPIGIYPDAIAKAAEQF
TDRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQAFERLLLNAPGWHGRVSLVQIAPPTRADVQTYQRIRQTLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGAL
VVNPFDLSQMAEALERALSMPLAERQARHADMMAPMRENNLSVWRDTFLADLRNVATASSVTAKAVKLADVTASS

Sequences:

>Translated_475_residues
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGETVSEPSAPVIEKQGNVTYATVGLTKRDYDQYYRGF
SNATLWPTFHYRNDLSRYDRQEYAGYQRVNATLAKQLKELLKPDDIIWVHDYHLLPFARCLRELGVKNPIGFFLHIPFPV
PEVLRTIPPHEELVKAMCSYDVIGFQTDADRQSFVDFIERGQHGTSSEDGMVHAYNRFLKVGAYPIGIYPDAIAKAAEQF
TDRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQAFERLLLNAPGWHGRVSLVQIAPPTRADVQTYQRIRQTLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGAL
VVNPFDLSQMAEALERALSMPLAERQARHADMMAPMRENNLSVWRDTFLADLRNVATASSVTAKAVKLADVTASS
>Mature_474_residues
SRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGETVSEPSAPVIEKQGNVTYATVGLTKRDYDQYYRGFS
NATLWPTFHYRNDLSRYDRQEYAGYQRVNATLAKQLKELLKPDDIIWVHDYHLLPFARCLRELGVKNPIGFFLHIPFPVP
EVLRTIPPHEELVKAMCSYDVIGFQTDADRQSFVDFIERGQHGTSSEDGMVHAYNRFLKVGAYPIGIYPDAIAKAAEQFT
DRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQAFERLLLNAPGWHGRVSLVQIAPPTRADVQTYQRIRQTLEGEAGR
INGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALV
VNPFDLSQMAEALERALSMPLAERQARHADMMAPMRENNLSVWRDTFLADLRNVATASSVTAKAVKLADVTASS

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=469, Percent_Identity=53.091684434968, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71987012, Length=440, Percent_Identity=28.8636363636364, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI115533558, Length=360, Percent_Identity=31.1111111111111, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI115533560, Length=360, Percent_Identity=31.1111111111111, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=33.6909871244635, Blast_Score=293, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6320279, Length=403, Percent_Identity=28.287841191067, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323537, Length=431, Percent_Identity=27.1461716937355, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323917, Length=398, Percent_Identity=27.1356783919598, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19920676, Length=483, Percent_Identity=35.8178053830228, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53295; Mature: 53164

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGETVSEPSAPVIEKQGN
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEECCCC
VTYATVGLTKRDYDQYYRGFSNATLWPTFHYRNDLSRYDRQEYAGYQRVNATLAKQLKEL
EEEEEECCCHHHHHHHHCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPDDIIWVHDYHLLPFARCLRELGVKNPIGFFLHIPFPVPEVLRTIPPHEELVKAMCSY
CCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHCC
DVIGFQTDADRQSFVDFIERGQHGTSSEDGMVHAYNRFLKVGAYPIGIYPDAIAKAAEQF
CCEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHH
TDRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQAFERLLLNAPGWHGRVSLVQIAPP
HCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRADVQTYQRIRQTLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTP
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
LRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALVVNPFDLSQMAEALERALSM
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCC
PLAERQARHADMMAPMRENNLSVWRDTFLADLRNVATASSVTAKAVKLADVTASS
CHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGETVSEPSAPVIEKQGN
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEECCCC
VTYATVGLTKRDYDQYYRGFSNATLWPTFHYRNDLSRYDRQEYAGYQRVNATLAKQLKEL
EEEEEECCCHHHHHHHHCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPDDIIWVHDYHLLPFARCLRELGVKNPIGFFLHIPFPVPEVLRTIPPHEELVKAMCSY
CCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHCC
DVIGFQTDADRQSFVDFIERGQHGTSSEDGMVHAYNRFLKVGAYPIGIYPDAIAKAAEQF
CCEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHH
TDRKPVRSLRDGMRGRKLIMSVDRLDYSKGLVERFQAFERLLLNAPGWHGRVSLVQIAPP
HCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRADVQTYQRIRQTLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTP
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
LRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALVVNPFDLSQMAEALERALSM
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCC
PLAERQARHADMMAPMRENNLSVWRDTFLADLRNVATASSVTAKAVKLADVTASS
CHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA