Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 91782358

GI number: 91782358

Start: 1094497

End: 1095405

Strand: Direct

Name: gcvA [H]

Synonym: Bxe_A3473

Alternate gene names: 91782358

Gene position: 1094497-1095405 (Clockwise)

Preceding gene: 91782355

Following gene: 91782365

Centisome position: 22.36

GC content: 64.47

Gene sequence:

>909_bases
ATGAAACGGGTATTGCCTCCGCTCAACGCATTGCGAGCCTTCGAGGCCGCAGGCCGGCTCGGCAGCTTCAAGGAAGCGGC
GGCCGAATTGCACGTGACTCATGGAGCGGTGAGTCAGCAGGTGCGGCTGCTCGAGGAATGGCTGGGCGCACCGCTATTCG
AGCGGCACAACCGGCGCGTGGTGCTCACCGCGGCGGCGAAGGCCTATCTGGGAGAAATCGGCCCACTGTTCGAGCAGTTG
TCGCAGGCAACCGCGAGATATGGTTTTCCGGAGACGGTCTCCCGCACACTTTCTGTGAATGCGCCGGCTACCTTCACATT
GCGCTGGCTGGTGCCGAGACTGGCGAAGTTCCGCGCCGGGCACCCGGACGTGGACGTGAAGGTGGAGACGTCGAACGAGT
CGGCGGAGAGCCTGAAAGATGTCTACGACATCGTTATCCGGGGTGGTCCCGACACGTTCTACGGCTACTCGATGCGGCCT
TTCCTGTCCGAGGAACGGCTGCCGGTCTGTAGTCCGGCGCTGTTGCAGCGACAGCCGCTTCGCACACCCGACGATCTGCG
CCAGCATACGTTGCTGCACACGTCGAGCCTTCCGCGGCTGTGGCCCGACTGGCTGGCGAGCGCGAAGATTCCCGCACTCA
GGCCGGCCGCCGCTCTGACCTTCGATCATTTCTATCTGACGTTGCAAGCCGCCATCGACGGGATAGGCATTGCGATGGGA
CCGACCGCGCTGGTTTCCGACGATCTCGCGGCCGGCCGGCTCGTCGCGCCGTTCGCCGGCCCTCGTCTGCCGTCGCGGAG
CTATTGCACGTATGTTCCGGATGGAAAGTCCGCGGATGACCTGGTCGCGCTGTTCCGCGCATGGCTCGAACGCGAAGGGA
TGCGCTTACCGGCGGAGACCAACCCCTGA

Upstream 100 bases:

>100_bases
CGTGCGGTGTCGGCTATCAGCACTACAAGCAGGATATCTATCTTGTCGCCCGCCAACAAACGAGTTAAATTGGGTCTTCG
CGTTAGATAAACTCTTGTGA

Downstream 100 bases:

>100_bases
CCGGAAACGCAGCCCCGCGTCGCGATACCCGGATTGCCGGAGCGGCCGGGCAGCGCGTGGCCCCGAGCGTCAGGTAAGCG
CGCTGCTGGCCGCGGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKRVLPPLNALRAFEAAGRLGSFKEAAAELHVTHGAVSQQVRLLEEWLGAPLFERHNRRVVLTAAAKAYLGEIGPLFEQL
SQATARYGFPETVSRTLSVNAPATFTLRWLVPRLAKFRAGHPDVDVKVETSNESAESLKDVYDIVIRGGPDTFYGYSMRP
FLSEERLPVCSPALLQRQPLRTPDDLRQHTLLHTSSLPRLWPDWLASAKIPALRPAAALTFDHFYLTLQAAIDGIGIAMG
PTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDGKSADDLVALFRAWLEREGMRLPAETNP

Sequences:

>Translated_302_residues
MKRVLPPLNALRAFEAAGRLGSFKEAAAELHVTHGAVSQQVRLLEEWLGAPLFERHNRRVVLTAAAKAYLGEIGPLFEQL
SQATARYGFPETVSRTLSVNAPATFTLRWLVPRLAKFRAGHPDVDVKVETSNESAESLKDVYDIVIRGGPDTFYGYSMRP
FLSEERLPVCSPALLQRQPLRTPDDLRQHTLLHTSSLPRLWPDWLASAKIPALRPAAALTFDHFYLTLQAAIDGIGIAMG
PTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDGKSADDLVALFRAWLEREGMRLPAETNP
>Mature_302_residues
MKRVLPPLNALRAFEAAGRLGSFKEAAAELHVTHGAVSQQVRLLEEWLGAPLFERHNRRVVLTAAAKAYLGEIGPLFEQL
SQATARYGFPETVSRTLSVNAPATFTLRWLVPRLAKFRAGHPDVDVKVETSNESAESLKDVYDIVIRGGPDTFYGYSMRP
FLSEERLPVCSPALLQRQPLRTPDDLRQHTLLHTSSLPRLWPDWLASAKIPALRPAAALTFDHFYLTLQAAIDGIGIAMG
PTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDGKSADDLVALFRAWLEREGMRLPAETNP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=306, Percent_Identity=36.2745098039216, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=33.5616438356164, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=32.312925170068, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=27.8745644599303, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=26.9076305220884, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787589, Length=310, Percent_Identity=26.1290322580645, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081978, Length=278, Percent_Identity=25.5395683453237, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=174, Percent_Identity=29.8850574712644, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=26.2068965517241, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787949, Length=63, Percent_Identity=53.968253968254, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33291; Mature: 33291

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVLPPLNALRAFEAAGRLGSFKEAAAELHVTHGAVSQQVRLLEEWLGAPLFERHNRRV
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEE
VLTAAAKAYLGEIGPLFEQLSQATARYGFPETVSRTLSVNAPATFTLRWLVPRLAKFRAG
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCEEHHHHHHHHHHHHCCC
HPDVDVKVETSNESAESLKDVYDIVIRGGPDTFYGYSMRPFLSEERLPVCSPALLQRQPL
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
RTPDDLRQHTLLHTSSLPRLWPDWLASAKIPALRPAAALTFDHFYLTLQAAIDGIGIAMG
CCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDGKSADDLVALFRAWLEREGMRLPAET
CHHHHCCCCCCCCEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
NP
CC
>Mature Secondary Structure
MKRVLPPLNALRAFEAAGRLGSFKEAAAELHVTHGAVSQQVRLLEEWLGAPLFERHNRRV
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEE
VLTAAAKAYLGEIGPLFEQLSQATARYGFPETVSRTLSVNAPATFTLRWLVPRLAKFRAG
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCEEHHHHHHHHHHHHCCC
HPDVDVKVETSNESAESLKDVYDIVIRGGPDTFYGYSMRPFLSEERLPVCSPALLQRQPL
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
RTPDDLRQHTLLHTSSLPRLWPDWLASAKIPALRPAAALTFDHFYLTLQAAIDGIGIAMG
CCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PTALVSDDLAAGRLVAPFAGPRLPSRSYCTYVPDGKSADDLVALFRAWLEREGMRLPAET
CHHHHCCCCCCCCEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
NP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]