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The map label for this gene is pcaQ [H]

Identifier: 91782290

GI number: 91782290

Start: 1017093

End: 1018352

Strand: Reverse

Name: pcaQ [H]

Synonym: Bxe_A3543

Alternate gene names: 91782290

Gene position: 1018352-1017093 (Counterclockwise)

Preceding gene: 91782292

Following gene: 91782289

Centisome position: 20.8

GC content: 58.1

Gene sequence:

>1260_bases
ATGGACCAGCTACCTAGAAAAGCCGCTCAGGCGCTAGATGAAATGACGAGCGTCTTTCATCTGAATTTGCGACATATTCA
CGGGCTGATAGAGGTCCATAAACACGGCAGCATCAGCGCCGCTGCGACGGCGGTCAGCCTGAGCCAGCCTGCACTGACGC
AGGGAATCCTGAAACTGGAAAGCCAGCTCGGAGAAGTGCTGTTCGAGCGCCGACCGGACGGCATCGTTCCTACGCTGGCG
GGCACGCTCGTGCTCGAGCGTGCCGCTGCGAGCATCGGCCATTTGACTGCCGGCTGCCGCCTCATCGCGGGCGCGGATTT
TTCGCCGGAGCGACGCCTGACGATGACGCAATTGCGCGCGTTCCTCGCGTTGTCGAAGGCAGGCAGCTTTGCGGCATCTG
CGATTGAACTGGGTTTGTCGCAGACTGCGGTGCATCGTGCCGTGCGTGAACTCGAAGACGCGCTGGGTCGCAGGCTCGTA
GAACGCCGAGGCCGAGGTGTACACATAAACTTTTGGGGACATCGTTTTGCGCGTTGCTGCTGGCTTGCGATCAAGGAACT
GCAAGCGGCACTATCTGAACTCGGTCTGGATCCGCTGAACCCGATGATTTCGGTTGGAGCCACGCCGCTTGCGCGGGCGT
TCCTCGTGCCAGAGGCCATGTCTTTGATGGTAACGGAGAAGTTTCCCGCCAGCTTTCGCGTGGTTGAAGGGAGTTGGGGG
GATTTGGTCGAGTCTTTACGTGATGGCGAAATCGATCTCATCGTTGGCGAACTGCCCGAGACAAGTGTTTCGGACCTTAA
GTGCACACGACTTTATACCGAGGCACCAGTCATCGTGGCTGGGCATCAGCATGATCTTGTCGCCAAGGGAAATCCGACCA
ATCAAACGCTTGCTTCATATCCTTGGATCATTGCCCCGGAGGCGTCGCCACTGCGCGCAGAATGGGATCGTCTGCTTCCT
GGAAAACGAGCACAAAAGCCTGTTGAATGCGGCTCGATTATGATCATCGGCCGGCTACTCACCTGCAGTGACATGCTAAC
GCTTGCGATGCCTGACCAGGTGGCGCTCCAGATTCGAAGCGGCTTACTCGCCCAAATCGGAAAGCCCTTGGCAAGCAGCA
TCACGATTGGAGCCACCATGCGCCATAGCTGGCGTCCTACCAGCGCACAGCGACGTTTTCTCAACCTTTTGCACATCGCC
TCAACAAGTCTCGGCGAGACCGCGAGCCGCAAACCGATGATTGAAGCTCCCTGGGTGTGA

Upstream 100 bases:

>100_bases
GAGCGGGATTCAAAGAATCCTTCAGTTATTCAATTGCCGAAAGTCTTGCCGCAACAACTGGTATATATAGACATTCACAG
CCACAGGCAGCCATCGAAAC

Downstream 100 bases:

>100_bases
GTCCACCCACTCGGAAGTTGCACTGCAAGAGTGCGGGTTCGACAATCCAATTCTGCTCGTCTGAATATGTTCCCGCGGAC
GAAGCCGGCCATCGACGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDQLPRKAAQALDEMTSVFHLNLRHIHGLIEVHKHGSISAAATAVSLSQPALTQGILKLESQLGEVLFERRPDGIVPTLA
GTLVLERAAASIGHLTAGCRLIAGADFSPERRLTMTQLRAFLALSKAGSFAASAIELGLSQTAVHRAVRELEDALGRRLV
ERRGRGVHINFWGHRFARCCWLAIKELQAALSELGLDPLNPMISVGATPLARAFLVPEAMSLMVTEKFPASFRVVEGSWG
DLVESLRDGEIDLIVGELPETSVSDLKCTRLYTEAPVIVAGHQHDLVAKGNPTNQTLASYPWIIAPEASPLRAEWDRLLP
GKRAQKPVECGSIMIIGRLLTCSDMLTLAMPDQVALQIRSGLLAQIGKPLASSITIGATMRHSWRPTSAQRRFLNLLHIA
STSLGETASRKPMIEAPWV

Sequences:

>Translated_419_residues
MDQLPRKAAQALDEMTSVFHLNLRHIHGLIEVHKHGSISAAATAVSLSQPALTQGILKLESQLGEVLFERRPDGIVPTLA
GTLVLERAAASIGHLTAGCRLIAGADFSPERRLTMTQLRAFLALSKAGSFAASAIELGLSQTAVHRAVRELEDALGRRLV
ERRGRGVHINFWGHRFARCCWLAIKELQAALSELGLDPLNPMISVGATPLARAFLVPEAMSLMVTEKFPASFRVVEGSWG
DLVESLRDGEIDLIVGELPETSVSDLKCTRLYTEAPVIVAGHQHDLVAKGNPTNQTLASYPWIIAPEASPLRAEWDRLLP
GKRAQKPVECGSIMIIGRLLTCSDMLTLAMPDQVALQIRSGLLAQIGKPLASSITIGATMRHSWRPTSAQRRFLNLLHIA
STSLGETASRKPMIEAPWV
>Mature_419_residues
MDQLPRKAAQALDEMTSVFHLNLRHIHGLIEVHKHGSISAAATAVSLSQPALTQGILKLESQLGEVLFERRPDGIVPTLA
GTLVLERAAASIGHLTAGCRLIAGADFSPERRLTMTQLRAFLALSKAGSFAASAIELGLSQTAVHRAVRELEDALGRRLV
ERRGRGVHINFWGHRFARCCWLAIKELQAALSELGLDPLNPMISVGATPLARAFLVPEAMSLMVTEKFPASFRVVEGSWG
DLVESLRDGEIDLIVGELPETSVSDLKCTRLYTEAPVIVAGHQHDLVAKGNPTNQTLASYPWIIAPEASPLRAEWDRLLP
GKRAQKPVECGSIMIIGRLLTCSDMLTLAMPDQVALQIRSGLLAQIGKPLASSITIGATMRHSWRPTSAQRRFLNLLHIA
STSLGETASRKPMIEAPWV

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=225, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=250, Percent_Identity=23.6, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789506, Length=236, Percent_Identity=25, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 45492; Mature: 45492

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLPRKAAQALDEMTSVFHLNLRHIHGLIEVHKHGSISAAATAVSLSQPALTQGILKLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
SQLGEVLFERRPDGIVPTLAGTLVLERAAASIGHLTAGCRLIAGADFSPERRLTMTQLRA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHH
FLALSKAGSFAASAIELGLSQTAVHRAVRELEDALGRRLVERRGRGVHINFWGHRFARCC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
WLAIKELQAALSELGLDPLNPMISVGATPLARAFLVPEAMSLMVTEKFPASFRVVEGSWG
HHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHH
DLVESLRDGEIDLIVGELPETSVSDLKCTRLYTEAPVIVAGHQHDLVAKGNPTNQTLASY
HHHHHHCCCCEEEEEECCCCCCHHHCCHHEEECCCCEEEECCCCCEEECCCCCCCHHHHC
PWIIAPEASPLRAEWDRLLPGKRAQKPVECGSIMIIGRLLTCSDMLTLAMPDQVALQIRS
CEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHH
GLLAQIGKPLASSITIGATMRHSWRPTSAQRRFLNLLHIASTSLGETASRKPMIEAPWV
HHHHHHCCHHHHCEEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDQLPRKAAQALDEMTSVFHLNLRHIHGLIEVHKHGSISAAATAVSLSQPALTQGILKLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
SQLGEVLFERRPDGIVPTLAGTLVLERAAASIGHLTAGCRLIAGADFSPERRLTMTQLRA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHH
FLALSKAGSFAASAIELGLSQTAVHRAVRELEDALGRRLVERRGRGVHINFWGHRFARCC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
WLAIKELQAALSELGLDPLNPMISVGATPLARAFLVPEAMSLMVTEKFPASFRVVEGSWG
HHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHH
DLVESLRDGEIDLIVGELPETSVSDLKCTRLYTEAPVIVAGHQHDLVAKGNPTNQTLASY
HHHHHHCCCCEEEEEECCCCCCHHHCCHHEEECCCCEEEECCCCCEEECCCCCCCHHHHC
PWIIAPEASPLRAEWDRLLPGKRAQKPVECGSIMIIGRLLTCSDMLTLAMPDQVALQIRS
CEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHH
GLLAQIGKPLASSITIGATMRHSWRPTSAQRRFLNLLHIASTSLGETASRKPMIEAPWV
HHHHHHCCHHHHCEEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]