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The map label for this gene is shiA [H]

Identifier: 91782257

GI number: 91782257

Start: 981728

End: 983041

Strand: Direct

Name: shiA [H]

Synonym: Bxe_A3577

Alternate gene names: 91782257

Gene position: 981728-983041 (Clockwise)

Preceding gene: 91782248

Following gene: 91782258

Centisome position: 20.05

GC content: 61.64

Gene sequence:

>1314_bases
ATGGATGTCCGGTCTACGACCAGCGGCGTATCGGGTGCATCTGCGTCCAGGATGCGAACCCGCGACTATATTCTGGCGGG
CTGGGCCAGTATGGCCGGCACGACGGTCGAGTGGTACGACTTCTTTCTGTACGGCACAGCTGCCGCGCTCATCTTCAACA
AGGTTTTCTTTCCCACGCTCGATCCGATTCTCGGCACGCTCGCGGCCTTCGCGACCTATGGAGTGGGCTTCATCGGCCGG
CCAATGGGAGGGATGGTGTTCGGCCACTTCGGCGACCGGATCGGGCGCAAGTCGATGTTGCTCGCCACATTGCTGCTGAT
GGGCATTCCGAGCATGGTGATCGGTCTGATTCCCTCGTATCAGAGCATCGGCTACTGGGCAGCGGTGTTGCTCGTGGCGA
TGCGTTTCCTGCAGGGTGTGGCCGTGGGCGGCGAATGGGGTGGGGCGGTCCTGATGGCCGTTGAGCATGCGCCACCGGGC
CGCAAGGGATTTTTCGGATCGCTGCCTCAAACGGGCGTGGGCTTCGGGCTTATTCTTTCGTCGATCGCGATGGCTACTGT
CACCGCGTTGCCCGAATCGGATTTGCTGTCGTGGGGCTGGCGTGTGCCGTTTCTCGCGAGCATCCTGCTCGTATTGGTTG
GGTGGGTGATCCGTGTGCGTGTGCCGGAGTCACCTGACTTCGAGCGGGTGAAGAGTCAGGGCCAGCCTGTGAAGGCACCC
GTGCTCGAAGTATTGCGCCGTCAGCCGCGCGAACTGCTAATCATCATCGGCGCGCGTACCGCTGAAAATACCTGGTTTTA
CCTGGTAGTGGCATTCGCATTGGCCTATGCCGCGAACCAGCTCAAGATTCCCAAGACGCAGATCCTGCACGCGATTACGG
CGGGGGCGGTGCTCTCGCTCGCGACGATGCCGTTCTCGGGCTGGCTGAGCGACCGTATCGGCCAGAAAGGCCTGTTCCTC
GCAGGCCTCGTGATTATGGGCCTGTTTGCCGCACCGTTCTTCTCGATGCTGGAAACGCTGCATCCAGGGATGGTCTGGTG
GGCGATGGTGCTCGGCGTGGGTGTTGTCTTCCCGATTCTCTATGCACCCGAGTCGCAGCTGTTTGCCGCGCAATTCCCCG
CTGAAATCCGTTATTCGGGCATCTCGGTGTCCGTGCAGGTGGCCGGCGTGCTGGGCGGCGGCGTCGCGCCGATGATCGCG
ACCGCACTGCTCGCATATGGCGGCGGCAGACCGCGTTACGTGGTGATCTATATGGTCGTGCTGGCCGTAATTGCTTTCCT
GTGCACGCTCATGATGCGTTCGCGGCACGATTGA

Upstream 100 bases:

>100_bases
TAACTATACTCGGCTGCAGGCCGCTGGACCAGAACGCGGTGTGAGGTGCTACGGGTGTTGGACTTCGGTCGCGCCAACAA
AAATGAAATGGAGACACAGT

Downstream 100 bases:

>100_bases
AAATTAAACCTGTAATCCTGTCTGAATGAGCGATGTGCGGCGAGGTCCGGATCGCTCGAACCTTTGAGAGAGAATTCATG
ATGAACGCAGTCACCCAGGC

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MDVRSTTSGVSGASASRMRTRDYILAGWASMAGTTVEWYDFFLYGTAAALIFNKVFFPTLDPILGTLAAFATYGVGFIGR
PMGGMVFGHFGDRIGRKSMLLATLLLMGIPSMVIGLIPSYQSIGYWAAVLLVAMRFLQGVAVGGEWGGAVLMAVEHAPPG
RKGFFGSLPQTGVGFGLILSSIAMATVTALPESDLLSWGWRVPFLASILLVLVGWVIRVRVPESPDFERVKSQGQPVKAP
VLEVLRRQPRELLIIIGARTAENTWFYLVVAFALAYAANQLKIPKTQILHAITAGAVLSLATMPFSGWLSDRIGQKGLFL
AGLVIMGLFAAPFFSMLETLHPGMVWWAMVLGVGVVFPILYAPESQLFAAQFPAEIRYSGISVSVQVAGVLGGGVAPMIA
TALLAYGGGRPRYVVIYMVVLAVIAFLCTLMMRSRHD

Sequences:

>Translated_437_residues
MDVRSTTSGVSGASASRMRTRDYILAGWASMAGTTVEWYDFFLYGTAAALIFNKVFFPTLDPILGTLAAFATYGVGFIGR
PMGGMVFGHFGDRIGRKSMLLATLLLMGIPSMVIGLIPSYQSIGYWAAVLLVAMRFLQGVAVGGEWGGAVLMAVEHAPPG
RKGFFGSLPQTGVGFGLILSSIAMATVTALPESDLLSWGWRVPFLASILLVLVGWVIRVRVPESPDFERVKSQGQPVKAP
VLEVLRRQPRELLIIIGARTAENTWFYLVVAFALAYAANQLKIPKTQILHAITAGAVLSLATMPFSGWLSDRIGQKGLFL
AGLVIMGLFAAPFFSMLETLHPGMVWWAMVLGVGVVFPILYAPESQLFAAQFPAEIRYSGISVSVQVAGVLGGGVAPMIA
TALLAYGGGRPRYVVIYMVVLAVIAFLCTLMMRSRHD
>Mature_437_residues
MDVRSTTSGVSGASASRMRTRDYILAGWASMAGTTVEWYDFFLYGTAAALIFNKVFFPTLDPILGTLAAFATYGVGFIGR
PMGGMVFGHFGDRIGRKSMLLATLLLMGIPSMVIGLIPSYQSIGYWAAVLLVAMRFLQGVAVGGEWGGAVLMAVEHAPPG
RKGFFGSLPQTGVGFGLILSSIAMATVTALPESDLLSWGWRVPFLASILLVLVGWVIRVRVPESPDFERVKSQGQPVKAP
VLEVLRRQPRELLIIIGARTAENTWFYLVVAFALAYAANQLKIPKTQILHAITAGAVLSLATMPFSGWLSDRIGQKGLFL
AGLVIMGLFAAPFFSMLETLHPGMVWWAMVLGVGVVFPILYAPESQLFAAQFPAEIRYSGISVSVQVAGVLGGGVAPMIA
TALLAYGGGRPRYVVIYMVVLAVIAFLCTLMMRSRHD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=42.4882629107981, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=37.0629370629371, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=32.4582338902148, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788942, Length=434, Percent_Identity=29.4930875576037, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI87082404, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082231, Length=205, Percent_Identity=25.3658536585366, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46966; Mature: 46966

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRSTTSGVSGASASRMRTRDYILAGWASMAGTTVEWYDFFLYGTAAALIFNKVFFPTL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DPILGTLAAFATYGVGFIGRPMGGMVFGHFGDRIGRKSMLLATLLLMGIPSMVIGLIPSY
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCH
QSIGYWAAVLLVAMRFLQGVAVGGEWGGAVLMAVEHAPPGRKGFFGSLPQTGVGFGLILS
HHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
SIAMATVTALPESDLLSWGWRVPFLASILLVLVGWVIRVRVPESPDFERVKSQGQPVKAP
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCHHH
VLEVLRRQPRELLIIIGARTAENTWFYLVVAFALAYAANQLKIPKTQILHAITAGAVLSL
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ATMPFSGWLSDRIGQKGLFLAGLVIMGLFAAPFFSMLETLHPGMVWWAMVLGVGVVFPIL
HHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YAPESQLFAAQFPAEIRYSGISVSVQVAGVLGGGVAPMIATALLAYGGGRPRYVVIYMVV
CCCCCHHHHHHCCHHHEECCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LAVIAFLCTLMMRSRHD
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVRSTTSGVSGASASRMRTRDYILAGWASMAGTTVEWYDFFLYGTAAALIFNKVFFPTL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DPILGTLAAFATYGVGFIGRPMGGMVFGHFGDRIGRKSMLLATLLLMGIPSMVIGLIPSY
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCH
QSIGYWAAVLLVAMRFLQGVAVGGEWGGAVLMAVEHAPPGRKGFFGSLPQTGVGFGLILS
HHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
SIAMATVTALPESDLLSWGWRVPFLASILLVLVGWVIRVRVPESPDFERVKSQGQPVKAP
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCHHH
VLEVLRRQPRELLIIIGARTAENTWFYLVVAFALAYAANQLKIPKTQILHAITAGAVLSL
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ATMPFSGWLSDRIGQKGLFLAGLVIMGLFAAPFFSMLETLHPGMVWWAMVLGVGVVFPIL
HHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YAPESQLFAAQFPAEIRYSGISVSVQVAGVLGGGVAPMIATALLAYGGGRPRYVVIYMVV
CCCCCHHHHHHCCHHHEECCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LAVIAFLCTLMMRSRHD
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]