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The map label for this gene is ntaB [H]

Identifier: 91782232

GI number: 91782232

Start: 952071

End: 952589

Strand: Direct

Name: ntaB [H]

Synonym: Bxe_A3603

Alternate gene names: 91782232

Gene position: 952071-952589 (Clockwise)

Preceding gene: 91782231

Following gene: 91782233

Centisome position: 19.45

GC content: 59.73

Gene sequence:

>519_bases
ATGAAAGACGAAGATAGAAAAACAGTGCGAGCGGTCCCGGACACGCGCAGTTTCCGGCGGGCGCTCGGCTGTTTTCCGAC
CGGCGTGACGATCGTGACTACCGCCGATTCATCGGGGATTCCGGCAGGCCTCACCTGCAATTCGTTCGGCTCCGTGTCGC
TCGAGCCACCATTGATCACGTGGAGTCTGCGTGCGACAAGCCGCTCGGCAGCAGTGTTCCGCCGCGCACGCGCTTTCGCG
GTCAACGTTCTGGCGGAAACGCAAAATCCTCTCGCGGTCCGTTTCGCCGACAGTTCGATTCCGGACAAATTCGCAACCGT
GCCGACGCGTCCCGGTCTGCGCAACATTCCGCTGATCGAAGATTGCGTTGCCTACTTCGAATGCGTGACCTGTACTGAAC
TCGATGTCGGTGACCACGTGCTTTTCATCGGCAACGTCGAACGTTTCAGCTACAGGGGACAGGACTCGCCACTCGTTTTC
TGCAAAGGCAATTACGCGGTGACCGCGGCGTGGCGCTGA

Upstream 100 bases:

>100_bases
CGATCCGGCACAACAGTACGGCGGCACGCATCAAGCCGATCTGAGTGCATTTGCGCGGCTTGGACGTTGAACGCGAGTTT
TCCTTATCAATGGAGAGGTC

Downstream 100 bases:

>100_bases
GGTCGCCATAGCGCGAAACGAACGGTTGACAACAGGATGGCGATGTCTAGAATGGTTTTACCATTATATCAGTGGAGGCG
AAAGATGGAGGCGATTGAAG

Product: putative oxygenase

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKDEDRKTVRAVPDTRSFRRALGCFPTGVTIVTTADSSGIPAGLTCNSFGSVSLEPPLITWSLRATSRSAAVFRRARAFA
VNVLAETQNPLAVRFADSSIPDKFATVPTRPGLRNIPLIEDCVAYFECVTCTELDVGDHVLFIGNVERFSYRGQDSPLVF
CKGNYAVTAAWR

Sequences:

>Translated_172_residues
MKDEDRKTVRAVPDTRSFRRALGCFPTGVTIVTTADSSGIPAGLTCNSFGSVSLEPPLITWSLRATSRSAAVFRRARAFA
VNVLAETQNPLAVRFADSSIPDKFATVPTRPGLRNIPLIEDCVAYFECVTCTELDVGDHVLFIGNVERFSYRGQDSPLVF
CKGNYAVTAAWR
>Mature_172_residues
MKDEDRKTVRAVPDTRSFRRALGCFPTGVTIVTTADSSGIPAGLTCNSFGSVSLEPPLITWSLRATSRSAAVFRRARAFA
VNVLAETQNPLAVRFADSSIPDKFATVPTRPGLRNIPLIEDCVAYFECVTCTELDVGDHVLFIGNVERFSYRGQDSPLVF
CKGNYAVTAAWR

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18796; Mature: 18796

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEDRKTVRAVPDTRSFRRALGCFPTGVTIVTTADSSGIPAGLTCNSFGSVSLEPPLIT
CCCCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCCEEE
WSLRATSRSAAVFRRARAFAVNVLAETQNPLAVRFADSSIPDKFATVPTRPGLRNIPLIE
EEECCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
DCVAYFECVTCTELDVGDHVLFIGNVERFSYRGQDSPLVFCKGNYAVTAAWR
HHHHHHHHHEEEEECCCCEEEEEECHHEEEECCCCCCEEEECCCEEEEEEEC
>Mature Secondary Structure
MKDEDRKTVRAVPDTRSFRRALGCFPTGVTIVTTADSSGIPAGLTCNSFGSVSLEPPLIT
CCCCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCEEECCCEEE
WSLRATSRSAAVFRRARAFAVNVLAETQNPLAVRFADSSIPDKFATVPTRPGLRNIPLIE
EEECCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHH
DCVAYFECVTCTELDVGDHVLFIGNVERFSYRGQDSPLVFCKGNYAVTAAWR
HHHHHHHHHEEEEECCCCEEEEEECHHEEEECCCCCCEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]