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The map label for this gene is cysD [H]

Identifier: 91782173

GI number: 91782173

Start: 888796

End: 889758

Strand: Direct

Name: cysD [H]

Synonym: Bxe_A3661

Alternate gene names: 91782173

Gene position: 888796-889758 (Clockwise)

Preceding gene: 91782172

Following gene: 91782174

Centisome position: 18.15

GC content: 60.23

Gene sequence:

>963_bases
ATGAGCACCACGCTCGATTCCACTGTGAACGCACCGCTAGCCAACACGGCAAACCGTATGGATCACCTCGATTGGCTCGA
AGCCGAGTCGATTCACATCCTGCGCGAACTGGTTGCCGAGTGCAGCAAGCCCGCGCTGCTGTTTTCGGGCGGCAAGGATT
CGGTCGTGGTGCTGCACCTTGCGCTGAAGGCCTTCGGTATCGGCGCGAATCGCAAGACCGTGCTGCCGTTCCCGCTCGTG
CATATCGACACCGGCCACAACTACGAGGAAGTGATCGACTTCCGCGATCGTCGCGCGAAAGAGATCGGCGCTGAACTCGT
GGTGGGTCACGTTGAAGATTCGATCAAGCGCGGCACGGTGCGTCTACGCCGTGAAACGGATTCGCGCAACGCCGCGCAAG
CCGTGACGCTGCTCGAAACCATCGAAGAGTACGGCTACACCGCGCTGATCGGCGGCGCGCGCCGCGATGAAGAAAAGGCC
CGTGCGAAAGAACGGATTTTCTCGTTCCGCGACGAATTCGGCCAGTGGGATCCGAAGGCGCAGCGCCCGGAACTGTGGAG
CCTGTACAACGCACGTCTGCATAACGGCGAACACCTGCGCGTGTTCCCGATTTCGAACTGGACCGAACTCGACGTGTGGC
AGTACATCGCCCGTGAAAAGCTCGAACTGCCGTCGATCTACTATGCGCATCAGCGCGAGATCGTGCGCCGCAATGGTCTG
CTCGTGCCGGTCACGCCGCTCACGCCGATGCGTGAAGGTGAAGTCAGTGAAACCGCGCTGGTGCGTTTCCGTACCGTCGG
CGATATCAGCTGCACGTGCCCGGTGGAAAGCGATGCAGATGATCTCGAGAAGATCATCGCCGAAACGGCCGTGACCGAAA
TCACGGAACGCGGTGCGACGCGGATGGACGATCAGGTCTCCGAAGCCGCGATGGAACAGCGTAAGAAGCAAGGTTATTTC
TAA

Upstream 100 bases:

>100_bases
AGCGGGCCTTGGCTTTTCGCCCGCACGCCGCACATATCGATTTGAATACTTGCGCGACGCGAGCAACCGCTTGCGACGCG
AACCAACGAAGGACCCAAAC

Downstream 100 bases:

>100_bases
GCACACGGACGAGGGCAAGACAATCATGAGCGGTATTCACCAACCAGAAGACCTCGGCGTGCTGCGTTTCATCACTGCGG
GCAGCGTCGACGATGGCAAG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTTLDSTVNAPLANTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGIGANRKTVLPFPLV
HIDTGHNYEEVIDFRDRRAKEIGAELVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEEYGYTALIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHQREIVRRNGL
LVPVTPLTPMREGEVSETALVRFRTVGDISCTCPVESDADDLEKIIAETAVTEITERGATRMDDQVSEAAMEQRKKQGYF

Sequences:

>Translated_320_residues
MSTTLDSTVNAPLANTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGIGANRKTVLPFPLV
HIDTGHNYEEVIDFRDRRAKEIGAELVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEEYGYTALIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHQREIVRRNGL
LVPVTPLTPMREGEVSETALVRFRTVGDISCTCPVESDADDLEKIIAETAVTEITERGATRMDDQVSEAAMEQRKKQGYF
>Mature_319_residues
STTLDSTVNAPLANTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGIGANRKTVLPFPLVH
IDTGHNYEEVIDFRDRRAKEIGAELVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEEYGYTALIGGARRDEEKAR
AKERIFSFRDEFGQWDPKAQRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHQREIVRRNGLL
VPVTPLTPMREGEVSETALVRFRTVGDISCTCPVESDADDLEKIIAETAVTEITERGATRMDDQVSEAAMEQRKKQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=46.7105263157895, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36317; Mature: 36185

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLDSTVNAPLANTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHEE
ALKAFGIGANRKTVLPFPLVHIDTGHNYEEVIDFRDRRAKEIGAELVVGHVEDSIKRGTV
HHHHHCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCE
RLRRETDSRNAAQAVTLLETIEEYGYTALIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMREGEVSETALVRFRTVGDISCTCPVESDADDLEKIIAETAVTEITERGAT
EEEECCCCCCCCCCCCHHHHEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQVSEAAMEQRKKQGYF
CCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLDSTVNAPLANTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHEE
ALKAFGIGANRKTVLPFPLVHIDTGHNYEEVIDFRDRRAKEIGAELVVGHVEDSIKRGTV
HHHHHCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCE
RLRRETDSRNAAQAVTLLETIEEYGYTALIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHNGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMREGEVSETALVRFRTVGDISCTCPVESDADDLEKIIAETAVTEITERGAT
EEEECCCCCCCCCCCCHHHHEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQVSEAAMEQRKKQGYF
CCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]