| Definition | Burkholderia xenovorans LB400 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007951 |
| Length | 4,895,836 |
Click here to switch to the map view.
The map label for this gene is capD [H]
Identifier: 91782064
GI number: 91782064
Start: 772344
End: 774221
Strand: Direct
Name: capD [H]
Synonym: Bxe_A3780
Alternate gene names: 91782064
Gene position: 772344-774221 (Clockwise)
Preceding gene: 91782063
Following gene: 91782069
Centisome position: 15.78
GC content: 58.04
Gene sequence:
>1878_bases ATGCTAAGAAGCAAGGCCGCTTGGCTGTCTTTCAGCGCTTTCCTTTTCGACTTATGCGCGGTCGCGGCCGCTTGGCTGAT CGCGTACATCATTCGTTTCAACGGCTTGGTTCCCGAAGATTTTCGGCTCGGGGCGATGCACTCGCTCGTCTGGGTCCTGC CGACGTACGGCATCATGTTCCGCATCTTCGGCTTGTACCGCGGCATGTGGGTGTTTGCGAGCTTGCCGGATCTCGTACGT ATATCGAAAGCAGTTATCGCCGGGGCGCTCGTCGCCATGGTCGTTTCGGTGCTGGTCCAACCTATGCCGATCGTGCCGCG CTCGGTGCTGGTGGTGTCGCCGCTATTCCTGTTTCTCGCGATGGGCGGATCGCGCGCGCTGTACCGTGCGACCAAAGAGT TCTACCTGTATGGCGGCCTCGTTGCGCAGGGTAAGCCGGTCGTTGTGCTTGGCGCCGGCAACGCGGGCGCGAGTCTCGCC CGCGAGCTGTCGCGTTCGAGCGAATGGCGTCTGGTGGGCTTGCTCGACGACGATGTCTCCAAGCATGGGCGCGAAATTTA CGGCTACAAGGTATTTGGCTCGATAAGCGAATTACCGCAATTGGCTGAGACGCTGAAAACTGAATACGCGATCATTGCCA TTCCGTCCGCTACAGTGGATGTACAGCGGCGTGTAGCGACGCTATGTGTGCGCGCCGGCGTACGGGCACTCGTGCTGCCT GCGCTAACCGACGTCACGCAAGGCCAGGCGTTTCTGTCGCGCGTGCGACAGATTGACCTCGAAGATTTGCTGGGCCGTGA TCCTATCAATATCGATACGCCCCATGTAGAAGCCCTGTTGCATGACAGAGTGGTGATGGTCACCGGCGCAGGCGGGTCTA TAGGCTCCGAATTGTGCCGCCAAATCTTGCGATTCCAGCCGGCTCAACTGATTGCTTATGACTTGTCCGAATTCGCGATC TATCGGCTTACTGAAGAGTTGAGCGAGCGTTTTCCAGGCGTGTCAGTAATTCCGGTTATCGGAGATGCGAAGGATTCGCT GCTGCTCGATGAGGTGATGTCGCGTCATACGCCGCACATCGTATTTCATGCGGCCGCGTATAAACACGTCCCGCTAATGG AAGAGCAGAACGCATGGCAGGCCGTGCGTAACAACGTGCTCGGCACGTACCGGGTAGGTCGCGCTGCGATTCGTCACGAT GTAAAGCATTTCGTGCTGATCTCCACCGACAAAGCAGTCAATCCGACCAACGTTATGGGTGCGAGTAAACGTCTTGCGGA AATGGCGTGTCAGGCCTTGCAGCAGACCAGCCCGCGCACCCAGTTCGAGACAGTTCGGTTCGGGAACGTACTCGGGAGCG CCGGGAGTGTCATACCGAAGTTCCAGCAGCAGATTGCAAAGGGCGGTCCCGTCACGGTGACGCACCCGGAGATCACGCGC TTCTTCATGACCATCCCGGAAGCGTCGCAACTGGTGCTACAAGCGTCCAGCATGGGGCACGGCGGCGAGATTTTCATCCT CGACATGGGCGAACCGGTAAAGATCGTCGACCTGGCTCGCGATTTGATTCGTTTGTACGGATTTAACGAGGAGCAGATCC GGATCGTGTTTACTGGTCTACGGCCTGGTGAAAAGCTCTACGAAGAACTGCTGGCTTGCGACGAGACGACAACACGTACA CCTCATCCTAAGTTGCGGACTGCGCGTGCGCGTGAAGTATCAGACGATCTGTTGGATAAGTTGTTGCCGTGGCTTATGCA GCATCGCGTGTTAGGCGACGACGAAGTACGCCGCGACTTGCGTCGCTGGGTGCCGGAATATCAACCGGCAAGCTCGCCGA CGCTTCACAGCGTCGCCGCGTTTCCCCGTGCTTCGTAG
Upstream 100 bases:
>100_bases GGCCGTTTGGATCGCCGTTCTCTGTGTAGCAGGCGCTTTGGTCGATTTGCGCTGGCGTCGTTTCCAATCAACACTGTCCG ATAACTGAGGTATAACGCTG
Downstream 100 bases:
>100_bases CGCTATGCGGAAGGGCGTCAGGAAATCACGATCTTGTGTACCATTCGCGCTTGATTTCGCGAGCGGCCTACGTCTTAAAA GGAAACCAGTTCGAGTGGTT
Product: putative epimerase/dehydratase polysaccharide- related biosynthesis protein
Products: UDPglucoseal [C]
Alternate protein names: NA
Number of amino acids: Translated: 625; Mature: 625
Protein sequence:
>625_residues MLRSKAAWLSFSAFLFDLCAVAAAWLIAYIIRFNGLVPEDFRLGAMHSLVWVLPTYGIMFRIFGLYRGMWVFASLPDLVR ISKAVIAGALVAMVVSVLVQPMPIVPRSVLVVSPLFLFLAMGGSRALYRATKEFYLYGGLVAQGKPVVVLGAGNAGASLA RELSRSSEWRLVGLLDDDVSKHGREIYGYKVFGSISELPQLAETLKTEYAIIAIPSATVDVQRRVATLCVRAGVRALVLP ALTDVTQGQAFLSRVRQIDLEDLLGRDPINIDTPHVEALLHDRVVMVTGAGGSIGSELCRQILRFQPAQLIAYDLSEFAI YRLTEELSERFPGVSVIPVIGDAKDSLLLDEVMSRHTPHIVFHAAAYKHVPLMEEQNAWQAVRNNVLGTYRVGRAAIRHD VKHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLARDLIRLYGFNEEQIRIVFTGLRPGEKLYEELLACDETTTRT PHPKLRTARAREVSDDLLDKLLPWLMQHRVLGDDEVRRDLRRWVPEYQPASSPTLHSVAAFPRAS
Sequences:
>Translated_625_residues MLRSKAAWLSFSAFLFDLCAVAAAWLIAYIIRFNGLVPEDFRLGAMHSLVWVLPTYGIMFRIFGLYRGMWVFASLPDLVR ISKAVIAGALVAMVVSVLVQPMPIVPRSVLVVSPLFLFLAMGGSRALYRATKEFYLYGGLVAQGKPVVVLGAGNAGASLA RELSRSSEWRLVGLLDDDVSKHGREIYGYKVFGSISELPQLAETLKTEYAIIAIPSATVDVQRRVATLCVRAGVRALVLP ALTDVTQGQAFLSRVRQIDLEDLLGRDPINIDTPHVEALLHDRVVMVTGAGGSIGSELCRQILRFQPAQLIAYDLSEFAI YRLTEELSERFPGVSVIPVIGDAKDSLLLDEVMSRHTPHIVFHAAAYKHVPLMEEQNAWQAVRNNVLGTYRVGRAAIRHD VKHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLARDLIRLYGFNEEQIRIVFTGLRPGEKLYEELLACDETTTRT PHPKLRTARAREVSDDLLDKLLPWLMQHRVLGDDEVRRDLRRWVPEYQPASSPTLHSVAAFPRAS >Mature_625_residues MLRSKAAWLSFSAFLFDLCAVAAAWLIAYIIRFNGLVPEDFRLGAMHSLVWVLPTYGIMFRIFGLYRGMWVFASLPDLVR ISKAVIAGALVAMVVSVLVQPMPIVPRSVLVVSPLFLFLAMGGSRALYRATKEFYLYGGLVAQGKPVVVLGAGNAGASLA RELSRSSEWRLVGLLDDDVSKHGREIYGYKVFGSISELPQLAETLKTEYAIIAIPSATVDVQRRVATLCVRAGVRALVLP ALTDVTQGQAFLSRVRQIDLEDLLGRDPINIDTPHVEALLHDRVVMVTGAGGSIGSELCRQILRFQPAQLIAYDLSEFAI YRLTEELSERFPGVSVIPVIGDAKDSLLLDEVMSRHTPHIVFHAAAYKHVPLMEEQNAWQAVRNNVLGTYRVGRAAIRHD VKHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSPRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLARDLIRLYGFNEEQIRIVFTGLRPGEKLYEELLACDETTTRT PHPKLRTARAREVSDDLLDKLLPWLMQHRVLGDDEVRRDLRRWVPEYQPASSPTLHSVAAFPRAS
Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]
COG id: COG1086
COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide synthase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016040 - InterPro: IPR003869 [H]
Pfam domain/function: PF02719 Polysacc_synt_2 [H]
EC number: 5.1.3.2 [C]
Molecular weight: Translated: 69186; Mature: 69186
Theoretical pI: Translated: 8.64; Mature: 8.64
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLRSKAAWLSFSAFLFDLCAVAAAWLIAYIIRFNGLVPEDFRLGAMHSLVWVLPTYGIMF CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH RIFGLYRGMWVFASLPDLVRISKAVIAGALVAMVVSVLVQPMPIVPRSVLVVSPLFLFLA HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH MGGSRALYRATKEFYLYGGLVAQGKPVVVLGAGNAGASLARELSRSSEWRLVGLLDDDVS CCCCHHHHHHHHHHHEECCEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHH KHGREIYGYKVFGSISELPQLAETLKTEYAIIAIPSATVDVQRRVATLCVRAGVRALVLP HCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHH ALTDVTQGQAFLSRVRQIDLEDLLGRDPINIDTPHVEALLHDRVVMVTGAGGSIGSELCR HHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHH QILRFQPAQLIAYDLSEFAIYRLTEELSERFPGVSVIPVIGDAKDSLLLDEVMSRHTPHI HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEE VFHAAAYKHVPLMEEQNAWQAVRNNVLGTYRVGRAAIRHDVKHFVLISTDKAVNPTNVMG EEEEHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH ASKRLAEMACQALQQTSPRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLARDLIRLYGFNEEQIRIVFTGL HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC RPGEKLYEELLACDETTTRTPHPKLRTARAREVSDDLLDKLLPWLMQHRVLGDDEVRRDL CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH RRWVPEYQPASSPTLHSVAAFPRAS HHHCCCCCCCCCCCHHHHHCCCCCC >Mature Secondary Structure MLRSKAAWLSFSAFLFDLCAVAAAWLIAYIIRFNGLVPEDFRLGAMHSLVWVLPTYGIMF CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH RIFGLYRGMWVFASLPDLVRISKAVIAGALVAMVVSVLVQPMPIVPRSVLVVSPLFLFLA HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH MGGSRALYRATKEFYLYGGLVAQGKPVVVLGAGNAGASLARELSRSSEWRLVGLLDDDVS CCCCHHHHHHHHHHHEECCEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHH KHGREIYGYKVFGSISELPQLAETLKTEYAIIAIPSATVDVQRRVATLCVRAGVRALVLP HCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHH ALTDVTQGQAFLSRVRQIDLEDLLGRDPINIDTPHVEALLHDRVVMVTGAGGSIGSELCR HHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHHH QILRFQPAQLIAYDLSEFAIYRLTEELSERFPGVSVIPVIGDAKDSLLLDEVMSRHTPHI HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEE VFHAAAYKHVPLMEEQNAWQAVRNNVLGTYRVGRAAIRHDVKHFVLISTDKAVNPTNVMG EEEEHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH ASKRLAEMACQALQQTSPRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH FFMTIPEASQLVLQASSMGHGGEIFILDMGEPVKIVDLARDLIRLYGFNEEQIRIVFTGL HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC RPGEKLYEELLACDETTTRTPHPKLRTARAREVSDDLLDKLLPWLMQHRVLGDDEVRRDL CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH RRWVPEYQPASSPTLHSVAAFPRAS HHHCCCCCCCCCCCHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 57600 [C]
Specific activity: 233.3
Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]
Substrates: UDPglucose [C]
Specific reaction: UDPglucose <==> UDPglucoseal [C]
General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7961465 [H]