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The map label for this gene is epsB [H]

Identifier: 91781994

GI number: 91781994

Start: 690982

End: 693336

Strand: Direct

Name: epsB [H]

Synonym: Bxe_A3850

Alternate gene names: 91781994

Gene position: 690982-693336 (Clockwise)

Preceding gene: 91781993

Following gene: 91781995

Centisome position: 14.11

GC content: 60.21

Gene sequence:

>2355_bases
ATGAATTCGAGTGTTTCCCGTACTCCGCCGGTTATGCTACGGAACAACGTAGATGCGTTTTCCATCGGCGAACTGGCGCG
GCATGTTGTGGACAATGTATGGCTGGTGATTGGCATTGTCGCCGTGGTAACAGGAGCGGCCGCGCTGTACGCATTCGTGG
CCACTCCCATTTATTCCGCGGATGCGTTGGTGAAAGTTGATTTTCCTCAGCCTAATGCGCTTGGTGAAGCGTCGAAGGCG
CAGGAGCAGGTCGTGCCGCCCACGCTGCCCACCGAAGCGGAGATTCAGATCATTCAGAGCCGGAGCGTGATTGCGCCGGT
GATCAGTAAATTCAGGCAGGACATCTCGATTACGCCGAATCGCATCCCGGTTATCGGCGCGCTCATCAGCCAATTCGCCG
CGCCGGGAACGCCGATGGCGGCACCCTTCGGTCTGGGCTCTTTCGCTTGGGGCGGAGAGGAAGCGTCCGTCGGTTCATTG
ACCGTGCCGGCCTGGCTCGAAGACGAATCACTCACGTTGACCGTTCTGCCCGGCGGACACTACAGGCTCACCGATCCAAA
AGGGCGAACCCTGCTAACGGGCGCACCTGGCGTACCGACCCAGGCCAACGGCGTGGCGCTCACGATGGATCGCCTCATCG
CGCGCCCCGGAACGGAATTCACGCTCGTGCGCTTTAGCGATGTCAAGGCAATCGAGCGTTTTCAGCATCAGTTGAAAGTC
AGCGAGTCGGGTAAGGACACCGGGGTCGTGCAAATCATCTACGAGAATCCCGATCCGGAACTGGCTCGCGCAATCGCCAA
CGGCATTGCCCAAGGCTATATCGCTTCTCATATCGCGCAGCATCGCGCCGAAGCGCTGACCACTCGCGAATTTATCGTGG
GCGAGTTGCCGCAGCTTCACGCTAACCTGACGCAGGCAGAAGCCGAACTGAGTCAGTTCAGAACTGCATCGAGCTCGATG
CAGGCGAGTACCGAAGCCGCGTCCTATCTCCAGGGCAGCATTGAGTTTGACCGTCAGATCGCAATGCTCAAGGTGCAGAA
AGCCGGTTTGGAAAGTCACTTTGCAGCGAACTCGCGCGACGTGCAGGCCATCGATAACCAGCTCCAGCTCCTTGAAAATG
CCAAGAGCGCTTTCGAGGCGCGCTTTGATCAATTACCCGCGTCCGAGCGACGGTCGGCTGACCTCACGCGCAACGTCAAG
GTCGCCGAGGATATCTATGTAGCGATGGTCAACAAATCCAACGAACTGGCGGTGACGCAGGCCGGAACGCTGGGGAACGT
ACATATCATCGACACCGCCCTGTTGCCCGTAGACCCTGTGAAGCCGAAGCGACCGCTGGTGATAGCCGTGGGCGCCGCTG
GCGGTCTGATCCTGGCGATCCTGTACGTCTTTATCCGGCGCCTGTCGTCGTCCGCGCTTGGGAACTCGATCGTGGCCGAG
CGCCATCTGAACCTGCCCGTCTTTGGTCACGTATTGTTCAGTGACGAGCAGGCGCGCCTTGACCGCGTGCAGAATTCGAA
CTCGCAGCTGGCGAAAAAAGGGCTTTCCGGTAAGGTGCAGCAGACAGTGGCCGCAAGCGCAACAGGATCGGCGCTCAGAT
TGACCTATAGCGGCGTTACTCCTCAAGACAGTATGTTGGCCGGCGGCCGCTTCCTGCTCGCCACTGACGGGTACGACGAC
ATACCGCTTGAGGCTTTGCGCGGTATACGCGCGACCTTGCAACGGCAGATTCAGGACGCGGCCAATAACGTGCTCGTGGT
AACGGGCGCGACGCCCGGCACGGGAAAGAGCTTTATCTCGTCGAATCTGGCCGTGCTCAGCGCCGAGGCCGGCAAACGGG
TGTTGCTGATCGACGGCGATATGCGCCGGGGCCAACTGGCCGCGATAATGAAGCAGACCGGTGCGGGCGGTCTCTCCGAA
GTTCTGACTGGCCGGATCGATGTCGACCACGTCATTCGAAACACAGATGTTCATGGACTTTCGTTCATCGCTGCTGGGCG
CTATCCGTCCAATCCGTCGCAGCTGCTCTCGACATCGCGCATGCAACAGCTACTCGAGCGACTTGGCGAATTGTATGACG
TGGTCATTGTCGACACTCCGCCCGTGCTGGCGGTGAGTGATGCAAACCTGATCGCCTCTCTTGCAGGATCGACTGTGCTC
GTGGTGCGTCCGGATGCCCAGAGCGACCGGGAGCTGGAGGAGGCCGCGCAACGTCTCGATCGCGCCGGCGCCCGTCTGGT
CGGCATGATATTCAACGCGATGCCGCGACGGCGCAGCGAGAAGCGCAGCTACGGCTATGCGGCTGCCTATTCCGCAGCGA
GCGTCCCACCCCAGCAACAGCATGGCGCCTCGTAA

Upstream 100 bases:

>100_bases
GATGCCTCATGCAATCCGATGCGCCAAGCGAGGCGCGCCGGGCGGAGAGGATTTGCCGCCAGTGGTTCGCTCTGGTTCTA
ATAGACAAGGAGTCCGTCGT

Downstream 100 bases:

>100_bases
TCGCTGCCGGCCACCGGCCGGCTGTCGTGCGTCCCTGCTTTGAGAAAACAATGTACCCCAGACTGGTATCCCTGCTGTTC
TACATGTTCTTTACCTGCGT

Product: protein-tyrosine kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 784; Mature: 784

Protein sequence:

>784_residues
MNSSVSRTPPVMLRNNVDAFSIGELARHVVDNVWLVIGIVAVVTGAAALYAFVATPIYSADALVKVDFPQPNALGEASKA
QEQVVPPTLPTEAEIQIIQSRSVIAPVISKFRQDISITPNRIPVIGALISQFAAPGTPMAAPFGLGSFAWGGEEASVGSL
TVPAWLEDESLTLTVLPGGHYRLTDPKGRTLLTGAPGVPTQANGVALTMDRLIARPGTEFTLVRFSDVKAIERFQHQLKV
SESGKDTGVVQIIYENPDPELARAIANGIAQGYIASHIAQHRAEALTTREFIVGELPQLHANLTQAEAELSQFRTASSSM
QASTEAASYLQGSIEFDRQIAMLKVQKAGLESHFAANSRDVQAIDNQLQLLENAKSAFEARFDQLPASERRSADLTRNVK
VAEDIYVAMVNKSNELAVTQAGTLGNVHIIDTALLPVDPVKPKRPLVIAVGAAGGLILAILYVFIRRLSSSALGNSIVAE
RHLNLPVFGHVLFSDEQARLDRVQNSNSQLAKKGLSGKVQQTVAASATGSALRLTYSGVTPQDSMLAGGRFLLATDGYDD
IPLEALRGIRATLQRQIQDAANNVLVVTGATPGTGKSFISSNLAVLSAEAGKRVLLIDGDMRRGQLAAIMKQTGAGGLSE
VLTGRIDVDHVIRNTDVHGLSFIAAGRYPSNPSQLLSTSRMQQLLERLGELYDVVIVDTPPVLAVSDANLIASLAGSTVL
VVRPDAQSDRELEEAAQRLDRAGARLVGMIFNAMPRRRSEKRSYGYAAAYSAASVPPQQQHGAS

Sequences:

>Translated_784_residues
MNSSVSRTPPVMLRNNVDAFSIGELARHVVDNVWLVIGIVAVVTGAAALYAFVATPIYSADALVKVDFPQPNALGEASKA
QEQVVPPTLPTEAEIQIIQSRSVIAPVISKFRQDISITPNRIPVIGALISQFAAPGTPMAAPFGLGSFAWGGEEASVGSL
TVPAWLEDESLTLTVLPGGHYRLTDPKGRTLLTGAPGVPTQANGVALTMDRLIARPGTEFTLVRFSDVKAIERFQHQLKV
SESGKDTGVVQIIYENPDPELARAIANGIAQGYIASHIAQHRAEALTTREFIVGELPQLHANLTQAEAELSQFRTASSSM
QASTEAASYLQGSIEFDRQIAMLKVQKAGLESHFAANSRDVQAIDNQLQLLENAKSAFEARFDQLPASERRSADLTRNVK
VAEDIYVAMVNKSNELAVTQAGTLGNVHIIDTALLPVDPVKPKRPLVIAVGAAGGLILAILYVFIRRLSSSALGNSIVAE
RHLNLPVFGHVLFSDEQARLDRVQNSNSQLAKKGLSGKVQQTVAASATGSALRLTYSGVTPQDSMLAGGRFLLATDGYDD
IPLEALRGIRATLQRQIQDAANNVLVVTGATPGTGKSFISSNLAVLSAEAGKRVLLIDGDMRRGQLAAIMKQTGAGGLSE
VLTGRIDVDHVIRNTDVHGLSFIAAGRYPSNPSQLLSTSRMQQLLERLGELYDVVIVDTPPVLAVSDANLIASLAGSTVL
VVRPDAQSDRELEEAAQRLDRAGARLVGMIFNAMPRRRSEKRSYGYAAAYSAASVPPQQQHGAS
>Mature_784_residues
MNSSVSRTPPVMLRNNVDAFSIGELARHVVDNVWLVIGIVAVVTGAAALYAFVATPIYSADALVKVDFPQPNALGEASKA
QEQVVPPTLPTEAEIQIIQSRSVIAPVISKFRQDISITPNRIPVIGALISQFAAPGTPMAAPFGLGSFAWGGEEASVGSL
TVPAWLEDESLTLTVLPGGHYRLTDPKGRTLLTGAPGVPTQANGVALTMDRLIARPGTEFTLVRFSDVKAIERFQHQLKV
SESGKDTGVVQIIYENPDPELARAIANGIAQGYIASHIAQHRAEALTTREFIVGELPQLHANLTQAEAELSQFRTASSSM
QASTEAASYLQGSIEFDRQIAMLKVQKAGLESHFAANSRDVQAIDNQLQLLENAKSAFEARFDQLPASERRSADLTRNVK
VAEDIYVAMVNKSNELAVTQAGTLGNVHIIDTALLPVDPVKPKRPLVIAVGAAGGLILAILYVFIRRLSSSALGNSIVAE
RHLNLPVFGHVLFSDEQARLDRVQNSNSQLAKKGLSGKVQQTVAASATGSALRLTYSGVTPQDSMLAGGRFLLATDGYDD
IPLEALRGIRATLQRQIQDAANNVLVVTGATPGTGKSFISSNLAVLSAEAGKRVLLIDGDMRRGQLAAIMKQTGAGGLSE
VLTGRIDVDHVIRNTDVHGLSFIAAGRYPSNPSQLLSTSRMQQLLERLGELYDVVIVDTPPVLAVSDANLIASLAGSTVL
VVRPDAQSDRELEEAAQRLDRAGARLVGMIFNAMPRRRSEKRSYGYAAAYSAASVPPQQQHGAS

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=749, Percent_Identity=31.1081441922563, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1787216, Length=766, Percent_Identity=30.2872062663185, Blast_Score=344, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 83614; Mature: 83614

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSVSRTPPVMLRNNVDAFSIGELARHVVDNVWLVIGIVAVVTGAAALYAFVATPIYSA
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DALVKVDFPQPNALGEASKAQEQVVPPTLPTEAEIQIIQSRSVIAPVISKFRQDISITPN
CCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
RIPVIGALISQFAAPGTPMAAPFGLGSFAWGGEEASVGSLTVPAWLEDESLTLTVLPGGH
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEEECCCC
YRLTDPKGRTLLTGAPGVPTQANGVALTMDRLIARPGTEFTLVRFSDVKAIERFQHQLKV
EEECCCCCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHH
SESGKDTGVVQIIYENPDPELARAIANGIAQGYIASHIAQHRAEALTTREFIVGELPQLH
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ANLTQAEAELSQFRTASSSMQASTEAASYLQGSIEFDRQIAMLKVQKAGLESHFAANSRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCH
VQAIDNQLQLLENAKSAFEARFDQLPASERRSADLTRNVKVAEDIYVAMVNKSNELAVTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHCCCEECCEEEEEEECCCCCEEEEE
AGTLGNVHIIDTALLPVDPVKPKRPLVIAVGAAGGLILAILYVFIRRLSSSALGNSIVAE
CCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RHLNLPVFGHVLFSDEQARLDRVQNSNSQLAKKGLSGKVQQTVAASATGSALRLTYSGVT
HCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCC
PQDSMLAGGRFLLATDGYDDIPLEALRGIRATLQRQIQDAANNVLVVTGATPGTGKSFIS
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
SNLAVLSAEAGKRVLLIDGDMRRGQLAAIMKQTGAGGLSEVLTGRIDVDHVIRNTDVHGL
CCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHCCCCCCE
SFIAAGRYPSNPSQLLSTSRMQQLLERLGELYDVVIVDTPPVLAVSDANLIASLAGSTVL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCHHHHHHHCCCEEE
VVRPDAQSDRELEEAAQRLDRAGARLVGMIFNAMPRRRSEKRSYGYAAAYSAASVPPQQQ
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCHH
HGAS
CCCC
>Mature Secondary Structure
MNSSVSRTPPVMLRNNVDAFSIGELARHVVDNVWLVIGIVAVVTGAAALYAFVATPIYSA
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DALVKVDFPQPNALGEASKAQEQVVPPTLPTEAEIQIIQSRSVIAPVISKFRQDISITPN
CCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
RIPVIGALISQFAAPGTPMAAPFGLGSFAWGGEEASVGSLTVPAWLEDESLTLTVLPGGH
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEEECCCC
YRLTDPKGRTLLTGAPGVPTQANGVALTMDRLIARPGTEFTLVRFSDVKAIERFQHQLKV
EEECCCCCCEEEECCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHH
SESGKDTGVVQIIYENPDPELARAIANGIAQGYIASHIAQHRAEALTTREFIVGELPQLH
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ANLTQAEAELSQFRTASSSMQASTEAASYLQGSIEFDRQIAMLKVQKAGLESHFAANSRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCH
VQAIDNQLQLLENAKSAFEARFDQLPASERRSADLTRNVKVAEDIYVAMVNKSNELAVTQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHCCCEECCEEEEEEECCCCCEEEEE
AGTLGNVHIIDTALLPVDPVKPKRPLVIAVGAAGGLILAILYVFIRRLSSSALGNSIVAE
CCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RHLNLPVFGHVLFSDEQARLDRVQNSNSQLAKKGLSGKVQQTVAASATGSALRLTYSGVT
HCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCC
PQDSMLAGGRFLLATDGYDDIPLEALRGIRATLQRQIQDAANNVLVVTGATPGTGKSFIS
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHH
SNLAVLSAEAGKRVLLIDGDMRRGQLAAIMKQTGAGGLSEVLTGRIDVDHVIRNTDVHGL
CCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHCCCCCCE
SFIAAGRYPSNPSQLLSTSRMQQLLERLGELYDVVIVDTPPVLAVSDANLIASLAGSTVL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCHHHHHHHCCCEEE
VVRPDAQSDRELEEAAQRLDRAGARLVGMIFNAMPRRRSEKRSYGYAAAYSAASVPPQQQ
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCHH
HGAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]