BacMap

Definition	Burkholderia xenovorans LB400 chromosome 1, complete sequence.
Accession	NC_007951
Length	4,895,836

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The map label for this gene is ydfG [H]

Identifier: 91781928

GI number: 91781928

Start: 612457

End: 613203

Strand: Reverse

Name: ydfG [H]

Synonym: Bxe_A3915

Alternate gene names: 91781928

Gene position: 613203-612457 (Counterclockwise)

Preceding gene: 91781935

Following gene: 91781927

Centisome position: 12.52

GC content: 65.6

Gene sequence:

>747_bases
ATGATCGTGTTCGTCACCGGCGCGTCCGCGGGATTCGGCGCCGCTATCGCGCGCGCCTTCGTCAAGGGCGGCCATCGTGT
CGTCGCCGCGGCACGCCGCAAAGACCGTCTGCAGGCGCTCGCCGACGAACTCGGCGACGCGCTTCTGCCGTACGAACTCG
ACGTGCGCGACCGCGCCGCCGTCGAAGCCGTGCCGGCCGCGCTGCCCGCGGACTTTGCCGCCATCGACGTGCTGGTCAAC
AATGCCGGCCTCGCACCGGACATGGAGCCGGCGCAAAAAGCGAGTCTCGACGCATGGACCGCCATGATCGAGACCAACTG
CACAGGTCTCGTACAGGTCACGCACACCCTGCTGCCCGGCATGGTGGAACGCAATCGCGGCCACATTTTCAATCTGGGCT
CGTCAGCGGCCAACTGGCCGTACGCGGGCGGCAACGTCTACGGAGCCACCAAGGCGTTCGTGCGTCAGTTCAGCCTGAAT
CTGCGCGCCGATCTGGCAGGAAGCGCGCTTCGGGTGACCGACATCGAGCCGGGCCTGTGCGGCGGCACCGAGTTCTCGAA
CGTGCGTTTTTGCGGCAAAGACGCCAGCGCCGCCAGAATGGTCGAAAACGTGCAACCGCTCACACCCGAAGACATCGCGG
ACTCGATCTACTGGATCGCCACCCGCCCGGCGCACGTCAATATCAACACGATCGAGCTGATGCCGGTGGCTCAATCGTTC
GCCGGTTTGACCGTTTATCGCGGCTGA

Upstream 100 bases:

>100_bases
CCTGCCGCAGCCTCTCGTACGGCTGGTTCATCCTTGCCGCAGCGCCGCCGTGGAAGTGAGTTTGGGATCTTTCTCTCCCG
CCGGCCGGGAAACGGCTGCC

Downstream 100 bases:

>100_bases
AGCCGGCCAGACGGGGCGCACATCTTGAAACAGACCTGGGGGGTGCGATCCGGTAAAATGCACCGCATGAATATATCGAC
CAGCCAGCCCAGCGCGGAAA

Product: putative short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVAAARRKDRLQALADELGDALLPYELDVRDRAAVEAVPAALPADFAAIDVLVN
NAGLAPDMEPAQKASLDAWTAMIETNCTGLVQVTHTLLPGMVERNRGHIFNLGSSAANWPYAGGNVYGATKAFVRQFSLN
LRADLAGSALRVTDIEPGLCGGTEFSNVRFCGKDASAARMVENVQPLTPEDIADSIYWIATRPAHVNINTIELMPVAQSF
AGLTVYRG

Sequences:

>Translated_248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVAAARRKDRLQALADELGDALLPYELDVRDRAAVEAVPAALPADFAAIDVLVN
NAGLAPDMEPAQKASLDAWTAMIETNCTGLVQVTHTLLPGMVERNRGHIFNLGSSAANWPYAGGNVYGATKAFVRQFSLN
LRADLAGSALRVTDIEPGLCGGTEFSNVRFCGKDASAARMVENVQPLTPEDIADSIYWIATRPAHVNINTIELMPVAQSF
AGLTVYRG
>Mature_248_residues
MIVFVTGASAGFGAAIARAFVKGGHRVVAAARRKDRLQALADELGDALLPYELDVRDRAAVEAVPAALPADFAAIDVLVN
NAGLAPDMEPAQKASLDAWTAMIETNCTGLVQVTHTLLPGMVERNRGHIFNLGSSAANWPYAGGNVYGATKAFVRQFSLN
LRADLAGSALRVTDIEPGLCGGTEFSNVRFCGKDASAARMVENVQPLTPEDIADSIYWIATRPAHVNINTIELMPVAQSF
AGLTVYRG

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=250, Percent_Identity=34, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI7706318, Length=188, Percent_Identity=35.1063829787234, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20149619, Length=186, Percent_Identity=31.1827956989247, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI22507405, Length=217, Percent_Identity=31.3364055299539, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI40548397, Length=179, Percent_Identity=32.4022346368715, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI214010158, Length=179, Percent_Identity=32.4022346368715, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI214010156, Length=179, Percent_Identity=32.4022346368715, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI22758144, Length=179, Percent_Identity=32.4022346368715, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI7705855, Length=200, Percent_Identity=28.5, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI19923817, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4557649, Length=178, Percent_Identity=29.2134831460674, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI150247226, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI7657478, Length=183, Percent_Identity=32.7868852459016, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157739938, Length=184, Percent_Identity=27.7173913043478, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19743808, Length=178, Percent_Identity=29.7752808988764, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI10190704, Length=228, Percent_Identity=28.5087719298246, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI226371731, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40254992, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI32483357, Length=232, Percent_Identity=26.2931034482759, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=64.7773279352227, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1787335, Length=179, Percent_Identity=31.8435754189944, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786812, Length=243, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082100, Length=179, Percent_Identity=32.4022346368715, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790717, Length=179, Percent_Identity=25.1396648044693, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=242, Percent_Identity=27.6859504132231, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=24.7011952191235, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=241, Percent_Identity=26.1410788381743, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=247, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=246, Percent_Identity=24.390243902439, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=241, Percent_Identity=26.1410788381743, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=242, Percent_Identity=24.7933884297521, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=184, Percent_Identity=26.6304347826087, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=23.6, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=181, Percent_Identity=32.0441988950276, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=167, Percent_Identity=28.1437125748503, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=167, Percent_Identity=28.1437125748503, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=255, Percent_Identity=32.9411764705882, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322227, Length=199, Percent_Identity=25.6281407035176, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322067, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116007236, Length=255, Percent_Identity=31.3725490196078, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281364494, Length=250, Percent_Identity=29.6, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24584748, Length=182, Percent_Identity=31.3186813186813, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI161076933, Length=182, Percent_Identity=31.3186813186813, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21358495, Length=223, Percent_Identity=29.1479820627803, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI116007238, Length=164, Percent_Identity=36.5853658536585, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357041, Length=182, Percent_Identity=30.2197802197802, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24641232, Length=260, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571526, Length=182, Percent_Identity=31.8681318681319, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI116007234, Length=255, Percent_Identity=30.5882352941176, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI23397609, Length=185, Percent_Identity=31.3513513513514, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574402, Length=183, Percent_Identity=31.1475409836066, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24584744, Length=183, Percent_Identity=31.1475409836066, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640442, Length=198, Percent_Identity=28.7878787878788, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24663911, Length=252, Percent_Identity=26.984126984127, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24643142, Length=176, Percent_Identity=30.1136363636364, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571387, Length=252, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24641388, Length=252, Percent_Identity=28.968253968254, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648693, Length=254, Percent_Identity=28.3464566929134, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21355889, Length=254, Percent_Identity=28.3464566929134, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24644339, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26268; Mature: 26268

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFVTGASAGFGAAIARAFVKGGHRVVAAARRKDRLQALADELGDALLPYELDVRDRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
VEAVPAALPADFAAIDVLVNNAGLAPDMEPAQKASLDAWTAMIETNCTGLVQVTHTLLPG
HHHHCCCCCCCHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCH
MVERNRGHIFNLGSSAANWPYAGGNVYGATKAFVRQFSLNLRADLAGSALRVTDIEPGLC
HHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCC
GGTEFSNVRFCGKDASAARMVENVQPLTPEDIADSIYWIATRPAHVNINTIELMPVAQSF
CCCCCCCEEEECCCCHHHHHHHHCCCCCHHHHHCCEEEEEECCCEEECEEEEEEEHHHHH
AGLTVYRG
CCEEEECC
>Mature Secondary Structure
MIVFVTGASAGFGAAIARAFVKGGHRVVAAARRKDRLQALADELGDALLPYELDVRDRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
VEAVPAALPADFAAIDVLVNNAGLAPDMEPAQKASLDAWTAMIETNCTGLVQVTHTLLPG
HHHHCCCCCCCHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCH
MVERNRGHIFNLGSSAANWPYAGGNVYGATKAFVRQFSLNLRADLAGSALRVTDIEPGLC
HHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCC
GGTEFSNVRFCGKDASAARMVENVQPLTPEDIADSIYWIATRPAHVNINTIELMPVAQSF
CCCCCCCEEEECCCCHHHHHHHHCCCCCHHHHHCCEEEEEECCCEEECEEEEEEEHHHHH
AGLTVYRG
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]