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The map label for this gene is ynfJ [C]

Identifier: 91781915

GI number: 91781915

Start: 598762

End: 600561

Strand: Reverse

Name: ynfJ [C]

Synonym: Bxe_A3930

Alternate gene names: 91781915

Gene position: 600561-598762 (Counterclockwise)

Preceding gene: 91781916

Following gene: 91781914

Centisome position: 12.27

GC content: 66.72

Gene sequence:

>1800_bases
ATGACAGCCGACACCCATAAGCGCGACTTCGCCGAAAACCGCCGCCTGCCCGGCATCGCTCTGCTGGCAGCGGGCATCGG
CGTAATCAGCACGCTGGCTGCTTTCGTGCTGCTGAATCTGATCCACCTGTTCACGAACCTGTTTTTCTTCGGCGGGCTTT
CGTTCGCGGAACGCTCGCCGGCGCAAAACACGCTCGGCGCCTGGGTCGTGCTGGTGCCGGTGGCGGGTGGTCTGATCGTC
GGGCTGATGGCGCGCTTCGGCTCGGAGAAGATTCGCGGCCACGGCATTCCGGAAGCGATCGAAGCCATTCTGTTCGGCAA
GAGCCGCATGTCGCCGAAGGTAGCGATCCTCAAGCCACTGTCCTCCGGCATCGTGATCGGCAGCGGTGGGCCGTTCGGCG
CGGAAGGTCCCATCATCATGACGGGCGGCGCGTTGGGTTCGCTGATTGCGCAGTGCGTGCGCGTGAGCGCGGCGGAGCGC
AAAACACTGCTGGTCGCCGGCGCCGCGGCCGGCATGACCGCGGTGTTCGGCACACCGGTCGCGGCGGTGCTCCTTGCGGT
CGAGCTGCTGTTGTTCGAATGGCGGCCGCGCAGCTTTCTGCCGGTCGCGCTCGCCTGTGCGGTGGCCGGCTTTGCGCGCG
GCGCCCTGTTCGGCGCGGGACCGTTGTTTCCGCTCGAAACGGCGCCGCCTGGCGCGCTCTCGCTGTTCACCTGCGTGATT
GCCGGCTTGCTCTCGGGAGCGCTGGCAGCAGGCCTCTCCGCCGCGCTCTACAAGACCGAAGACCTGTTCGGCAAACTCAG
GATCCACTGGATGTGGTGGCCCGCGCTCGGCGGACTGGTGGTCGGCATTGGCGGCTTTATCGAGCCGCGTGCGCTGGGCG
TCGGCTACGACGTCATCGGTGACCTGCTGCATCTGCATCTGGCCTTGGAGATTGCGCTTGCGTTGCTGCTCGTCAAGGCC
GTCATGTGGGTCGTCGCGCTCGGCTCGGGTACGTCGGGCGGCGTGCTGGCGCCGTTGCTGATGCTCGGCGCCGGTCTCGG
CGTGGTGTTGAGCCATCTGATGCCGGGCGGCGATCCGGCACTGTGGCCGCTCGTGTGCATGGCCGCCACGCTCGGCGCGA
CGCTTGGGGCGCCGCTCACCGCAATCGTGTTCGCGTTCGGCCTGACCAACGACAGCAACGCGTTATTGCCGCTACTCGCC
GCCACGCTGGTCGCACATGGCTTCGCGACGGTCGTGATGCGACGCTCGATCATGACCGAGAAAATCGCGCGCCGCGGGTA
TCACATCTACCGCGAATACGGCGTCGATCCACTGGAGCGCCATTACGTCGACGAAGTCATGACACGCGACGTCGAAACGA
TCGACGCACACTCGACAGTCGACGAAACGCTGACGCGGTACTTCGGCCCTGGGCAAACGCATCGCGCGTATCCGGTGATC
GTGGACGGCGCGGTGACCGGGGTGATCGACCGCGCGGCCCTCGAACGCATTCCTCGCACTCGCGACCGCCAGCCCGGCGC
GACTACATTGCTCGAACTGATGGAGACGCGCGCGCCTGTCGTCGCGTTGCCCGGCGAGACCTGCCGGCTCGTTGCAACAC
GCCTCGCGGTTCATGGCCTGGAGCGCATGCCCGTGGTGAGCGACATGAACACGCTGCGAATCGTCGGCATCGTGGCGCGC
AGCGACCTGGTGAAACCGTCGCTCGCGCATTTCGACGAAGAGCATAAGAAGGAGCGCTTCCGGCGTCTCGGCACGCGTGC
GGCGAAGCGTCGCCTTTCGCCGCTTCGCCGCAGCGGCTGA

Upstream 100 bases:

>100_bases
CGAACTGAAGTCGCTCGAAGGCGCGTTCAACGTGCCGCAGATCGATCTCTAGCGGGCGGCACGCCGCTCACTGGCTACTC
ACCTCAACCTTGTCTCGATA

Downstream 100 bases:

>100_bases
CATGAATCTTTAAGCGCAGGTGTCGATCCACACTGCGTAGGAAAGCGTAATCACAGATTTTTCACAAAATGCTTTCCACC
ATTTCGCACGACCCGTTAAG

Product: voltage-gated chloride channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTADTHKRDFAENRRLPGIALLAAGIGVISTLAAFVLLNLIHLFTNLFFFGGLSFAERSPAQNTLGAWVVLVPVAGGLIV
GLMARFGSEKIRGHGIPEAIEAILFGKSRMSPKVAILKPLSSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVRVSAAER
KTLLVAGAAAGMTAVFGTPVAAVLLAVELLLFEWRPRSFLPVALACAVAGFARGALFGAGPLFPLETAPPGALSLFTCVI
AGLLSGALAAGLSAALYKTEDLFGKLRIHWMWWPALGGLVVGIGGFIEPRALGVGYDVIGDLLHLHLALEIALALLLVKA
VMWVVALGSGTSGGVLAPLLMLGAGLGVVLSHLMPGGDPALWPLVCMAATLGATLGAPLTAIVFAFGLTNDSNALLPLLA
ATLVAHGFATVVMRRSIMTEKIARRGYHIYREYGVDPLERHYVDEVMTRDVETIDAHSTVDETLTRYFGPGQTHRAYPVI
VDGAVTGVIDRAALERIPRTRDRQPGATTLLELMETRAPVVALPGETCRLVATRLAVHGLERMPVVSDMNTLRIVGIVAR
SDLVKPSLAHFDEEHKKERFRRLGTRAAKRRLSPLRRSG

Sequences:

>Translated_599_residues
MTADTHKRDFAENRRLPGIALLAAGIGVISTLAAFVLLNLIHLFTNLFFFGGLSFAERSPAQNTLGAWVVLVPVAGGLIV
GLMARFGSEKIRGHGIPEAIEAILFGKSRMSPKVAILKPLSSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVRVSAAER
KTLLVAGAAAGMTAVFGTPVAAVLLAVELLLFEWRPRSFLPVALACAVAGFARGALFGAGPLFPLETAPPGALSLFTCVI
AGLLSGALAAGLSAALYKTEDLFGKLRIHWMWWPALGGLVVGIGGFIEPRALGVGYDVIGDLLHLHLALEIALALLLVKA
VMWVVALGSGTSGGVLAPLLMLGAGLGVVLSHLMPGGDPALWPLVCMAATLGATLGAPLTAIVFAFGLTNDSNALLPLLA
ATLVAHGFATVVMRRSIMTEKIARRGYHIYREYGVDPLERHYVDEVMTRDVETIDAHSTVDETLTRYFGPGQTHRAYPVI
VDGAVTGVIDRAALERIPRTRDRQPGATTLLELMETRAPVVALPGETCRLVATRLAVHGLERMPVVSDMNTLRIVGIVAR
SDLVKPSLAHFDEEHKKERFRRLGTRAAKRRLSPLRRSG
>Mature_598_residues
TADTHKRDFAENRRLPGIALLAAGIGVISTLAAFVLLNLIHLFTNLFFFGGLSFAERSPAQNTLGAWVVLVPVAGGLIVG
LMARFGSEKIRGHGIPEAIEAILFGKSRMSPKVAILKPLSSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVRVSAAERK
TLLVAGAAAGMTAVFGTPVAAVLLAVELLLFEWRPRSFLPVALACAVAGFARGALFGAGPLFPLETAPPGALSLFTCVIA
GLLSGALAAGLSAALYKTEDLFGKLRIHWMWWPALGGLVVGIGGFIEPRALGVGYDVIGDLLHLHLALEIALALLLVKAV
MWVVALGSGTSGGVLAPLLMLGAGLGVVLSHLMPGGDPALWPLVCMAATLGATLGAPLTAIVFAFGLTNDSNALLPLLAA
TLVAHGFATVVMRRSIMTEKIARRGYHIYREYGVDPLERHYVDEVMTRDVETIDAHSTVDETLTRYFGPGQTHRAYPVIV
DGAVTGVIDRAALERIPRTRDRQPGATTLLELMETRAPVVALPGETCRLVATRLAVHGLERMPVVSDMNTLRIVGIVARS
DLVKPSLAHFDEEHKKERFRRLGTRAAKRRLSPLRRSG

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=416, Percent_Identity=29.3269230769231, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1786350, Length=320, Percent_Identity=26.25, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 63255; Mature: 63123

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADTHKRDFAENRRLPGIALLAAGIGVISTLAAFVLLNLIHLFTNLFFFGGLSFAERSP
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
AQNTLGAWVVLVPVAGGLIVGLMARFGSEKIRGHGIPEAIEAILFGKSRMSPKVAILKPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEC
SSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVRVSAAERKTLLVAGAAAGMTAVFGTPV
CCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECHHCHHHHHCCHH
AAVLLAVELLLFEWRPRSFLPVALACAVAGFARGALFGAGPLFPLETAPPGALSLFTCVI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AGLLSGALAAGLSAALYKTEDLFGKLRIHWMWWPALGGLVVGIGGFIEPRALGVGYDVIG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCCCCCHHHCCCHHHHH
DLLHLHLALEIALALLLVKAVMWVVALGSGTSGGVLAPLLMLGAGLGVVLSHLMPGGDPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCH
LWPLVCMAATLGATLGAPLTAIVFAFGLTNDSNALLPLLAATLVAHGFATVVMRRSIMTE
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIARRGYHIYREYGVDPLERHYVDEVMTRDVETIDAHSTVDETLTRYFGPGQTHRAYPVI
HHHHCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VDGAVTGVIDRAALERIPRTRDRQPGATTLLELMETRAPVVALPGETCRLVATRLAVHGL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHH
ERMPVVSDMNTLRIVGIVARSDLVKPSLAHFDEEHKKERFRRLGTRAAKRRLSPLRRSG
HCCCCCCCCCHHHHEEEHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TADTHKRDFAENRRLPGIALLAAGIGVISTLAAFVLLNLIHLFTNLFFFGGLSFAERSP
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
AQNTLGAWVVLVPVAGGLIVGLMARFGSEKIRGHGIPEAIEAILFGKSRMSPKVAILKPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEC
SSGIVIGSGGPFGAEGPIIMTGGALGSLIAQCVRVSAAERKTLLVAGAAAGMTAVFGTPV
CCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECHHCHHHHHCCHH
AAVLLAVELLLFEWRPRSFLPVALACAVAGFARGALFGAGPLFPLETAPPGALSLFTCVI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AGLLSGALAAGLSAALYKTEDLFGKLRIHWMWWPALGGLVVGIGGFIEPRALGVGYDVIG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCCCCCHHHCCCHHHHH
DLLHLHLALEIALALLLVKAVMWVVALGSGTSGGVLAPLLMLGAGLGVVLSHLMPGGDPA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCH
LWPLVCMAATLGATLGAPLTAIVFAFGLTNDSNALLPLLAATLVAHGFATVVMRRSIMTE
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIARRGYHIYREYGVDPLERHYVDEVMTRDVETIDAHSTVDETLTRYFGPGQTHRAYPVI
HHHHCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VDGAVTGVIDRAALERIPRTRDRQPGATTLLELMETRAPVVALPGETCRLVATRLAVHGL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHH
ERMPVVSDMNTLRIVGIVARSDLVKPSLAHFDEEHKKERFRRLGTRAAKRRLSPLRRSG
HCCCCCCCCCHHHHEEEHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]