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The map label for this gene is mscS [H]

Identifier: 91781913

GI number: 91781913

Start: 597074

End: 597886

Strand: Reverse

Name: mscS [H]

Synonym: Bxe_A3932

Alternate gene names: 91781913

Gene position: 597886-597074 (Counterclockwise)

Preceding gene: 91781914

Following gene: 91781911

Centisome position: 12.21

GC content: 62.73

Gene sequence:

>813_bases
TTGGATCTCGAAACCGTACGCGTCTTCATCATGACCCGAGGTATCGACCTCGGCACCAAGGTCGTCGGGGCGATCGTCTT
GTGGATCGTCGGCCGTTGGCTCATCGGCATGATCACCGGCTTGCTGCGCAAGATGTTGTCGCGCAACGGCCGGGTCGATC
CCACGCTGGCGCATTATCTCGGTTCGATTCTCGGCGCATTGCTGAACCTGCTCCTGATCCTCGCGATCCTGCAGGTGTTC
GGTGTGCAAACCACTTCGTTCGCCGCGCTGCTCGCCGGCCTCGGCCTCGCAATCGGCACCGCCTGGGGCGGCCTGCTTGC
CCACTTCGCCGCAGGCGTATTCATGCAGGTATTGCGGCCGTTCAAGGTCGGCGATTTCGTCACCGCGGGCGGCGTCACCG
GCACGGTCTCGGAACTCGGCCTGTTCGGCACCACCATCGTGACGCCGGACAACGTGACGACTATCGTCGGCAATAACAAG
ATCTTCTCCGACACGATTTCGAACTACAGCATCCTGCCCGTGCGCCGCGTCGAGCTGACGGCGAAGATCGCCAACGGCGT
CGATCCGACGGATGCGATGAACCGGTTGAAGGCGGCCGTCACGCAGATTCCGAACGTGGCCGAGAGTCCCGCACCGGACA
TCGAAGTACTCGGCTTCACGCCGGAAGGTCCGCTGCTTTGCGTGCGGCCGTACACGCACAATGCGAACTATTGGCAGGTC
TACTTCGACACCAATCGCGCGATCATCCAGACTTTCAAGGAAGCCGGCTACCCGACGCCGGAAACACCGCTGGCGCCGCG
CGCGGTGACGTGA

Upstream 100 bases:

>100_bases
GACCGAGTGTTACCGTTTGCGCCCGTCGCCGGGCATTTGCCGCTGTATTGCACCACCGCAGTCCAATCAGTCGAAGGCAA
CTCACCCAGCGAGGAACATC

Downstream 100 bases:

>100_bases
GTATCGTCGTTTGATGGAAGTCGCGCCCAACTGAACGACGCGGCTTCATCGTGTCGTCAATTGTTAAATCCACAATCAGA
GGTGCAGAACGAAAACGGCG

Product: small conductance mechanosensitive Ion channel, (MscS) family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDLETVRVFIMTRGIDLGTKVVGAIVLWIVGRWLIGMITGLLRKMLSRNGRVDPTLAHYLGSILGALLNLLLILAILQVF
GVQTTSFAALLAGLGLAIGTAWGGLLAHFAAGVFMQVLRPFKVGDFVTAGGVTGTVSELGLFGTTIVTPDNVTTIVGNNK
IFSDTISNYSILPVRRVELTAKIANGVDPTDAMNRLKAAVTQIPNVAESPAPDIEVLGFTPEGPLLCVRPYTHNANYWQV
YFDTNRAIIQTFKEAGYPTPETPLAPRAVT

Sequences:

>Translated_270_residues
MDLETVRVFIMTRGIDLGTKVVGAIVLWIVGRWLIGMITGLLRKMLSRNGRVDPTLAHYLGSILGALLNLLLILAILQVF
GVQTTSFAALLAGLGLAIGTAWGGLLAHFAAGVFMQVLRPFKVGDFVTAGGVTGTVSELGLFGTTIVTPDNVTTIVGNNK
IFSDTISNYSILPVRRVELTAKIANGVDPTDAMNRLKAAVTQIPNVAESPAPDIEVLGFTPEGPLLCVRPYTHNANYWQV
YFDTNRAIIQTFKEAGYPTPETPLAPRAVT
>Mature_270_residues
MDLETVRVFIMTRGIDLGTKVVGAIVLWIVGRWLIGMITGLLRKMLSRNGRVDPTLAHYLGSILGALLNLLLILAILQVF
GVQTTSFAALLAGLGLAIGTAWGGLLAHFAAGVFMQVLRPFKVGDFVTAGGVTGTVSELGLFGTTIVTPDNVTTIVGNNK
IFSDTISNYSILPVRRVELTAKIANGVDPTDAMNRLKAAVTQIPNVAESPAPDIEVLGFTPEGPLLCVRPYTHNANYWQV
YFDTNRAIIQTFKEAGYPTPETPLAPRAVT

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=256, Percent_Identity=32.03125, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786670, Length=201, Percent_Identity=24.8756218905473, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367355, Length=255, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 28934; Mature: 28934

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLETVRVFIMTRGIDLGTKVVGAIVLWIVGRWLIGMITGLLRKMLSRNGRVDPTLAHYL
CCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GSILGALLNLLLILAILQVFGVQTTSFAALLAGLGLAIGTAWGGLLAHFAAGVFMQVLRP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKVGDFVTAGGVTGTVSELGLFGTTIVTPDNVTTIVGNNKIFSDTISNYSILPVRRVELT
CCCCCEEECCCCCCCHHHHHHCCCEEECCCCEEEEECCCEEEHHHCCCCEECEEHHHHHH
AKIANGVDPTDAMNRLKAAVTQIPNVAESPAPDIEVLGFTPEGPLLCVRPYTHNANYWQV
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEE
YFDTNRAIIQTFKEAGYPTPETPLAPRAVT
EEECCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLETVRVFIMTRGIDLGTKVVGAIVLWIVGRWLIGMITGLLRKMLSRNGRVDPTLAHYL
CCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GSILGALLNLLLILAILQVFGVQTTSFAALLAGLGLAIGTAWGGLLAHFAAGVFMQVLRP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKVGDFVTAGGVTGTVSELGLFGTTIVTPDNVTTIVGNNKIFSDTISNYSILPVRRVELT
CCCCCEEECCCCCCCHHHHHHCCCEEECCCCEEEEECCCEEEHHHCCCCEECEEHHHHHH
AKIANGVDPTDAMNRLKAAVTQIPNVAESPAPDIEVLGFTPEGPLLCVRPYTHNANYWQV
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEE
YFDTNRAIIQTFKEAGYPTPETPLAPRAVT
EEECCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]