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The map label for this gene is mbeA [H]

Identifier: 91781907

GI number: 91781907

Start: 589351

End: 590610

Strand: Direct

Name: mbeA [H]

Synonym: Bxe_A3938

Alternate gene names: 91781907

Gene position: 589351-590610 (Clockwise)

Preceding gene: 91781906

Following gene: 91781908

Centisome position: 12.04

GC content: 38.57

Gene sequence:

>1260_bases
ATGCTAGCGAACTGTTTTGATTCTGGAACCAGTGGAGGTGATGCACCTGTTCGATATCTTTTAGGCAATAAAGACCATAA
AGGAAGCGAACGAAGTGTTAAGCCAAAATTATTTCGAGGCGATCCAGAATCCACGAAAGACTTGATAGATAGTATAAATA
ATAAACAAAAATATACTTCAGGAGTCCTTGCATTTCGTGAAAATGAAGTCCCTTCTGTCAAACAAATGCACCAAATTTGT
GACGAGTTTGAGCGCTATATGTTTGGTGGGTTAAGTAAAGATAGAGTTAATATTTTATGGGTGCTTCATAATGATAAGGG
TCGTCCTGAATTGCATTTTGTTGTGCCAAAAATAGACCTAAAATCGGGTAAGCAACTAAACATTTCTCCACCTGGTAAGC
AAAATCAAGACTACTATCGATTGTTTGGTGCTTACATTAATGACAAGTTTGGATGGGAGCAAGTTAGTCCAAAAAAGAAG
TGGGATTTGAAAGCTGCTGAGTATAAAAGTGAAACTGTTCCAGCTTTATACAAAAGAGCACTTTGCAAGCAAATCAACAA
TAATTATGAAACAGGGATGTTTAAGGATAGAAATGATTTGATCAAATGGCTTGAGCAAAATAAAATTCAGGTTCTAAAGC
AAGCTGATGACTTCATAACAATTGGAGTGGGTGGCAATAAACTACGCATGTATGGGGAAATATTTAAGCAAAATCCAGAT
ATGTCAGTAGGGAAACCCTATGTGTTCAATCAACATGAAAAAAACAGATTGGAGCATTATAGAGTTGAGCGCGTAGCTTA
CTTTGATAAGCGTTTTAATACTCCCCCAAAACCTAGATTTACAGCATCTCCACAAGGCAAAACAACTGTAAAGCCTCAAG
TTTTCCAGACTGTAGCCGATAGAATTAAGGAAAGAGAGGTCGCTAGCATGGTTCAAAACATGGCTAGCATTGGGGGTGAT
GTAGCTGATGTGATGCCAAAAGAGCAAAAGCCATCATCTGGCGTGTCTTCTGGTGGGGGTTCTTCTGGTGGGGCTGATAC
TTCACTTCAAAAAGTAGCGCTTTCTTTAAGAAGAAACGATATTTTGAGGCAGTTAATTGATGCAACGCCTTCCCAAGTGG
CTGAATTGCAAAATGAGCTTACTAATATAGATATTGCTTTGGGACGCTTGAAATTGCAGGAAATGGAAGAGGCTGGGCGT
GAAATGGATAAACTTTATGAGTTATATAAAAAGCCTCAGATAGATGAGGCACCTACTTAA

Upstream 100 bases:

>100_bases
CTAATTTACTTCATATGTCACTTTTACATGAGGTAAGTGACATAGCAAAAACGCTCAAGCAGTTGAGGAAAAACTTTGAT
AAAGGTGTTGAGATTTAAGG

Downstream 100 bases:

>100_bases
AATGTAATAAATTGCGACTAAATAGTGACATATCATGTTCACGATGTAAAATATACATGCATACTAATAAAGGAGAAAAA
AATGCACAATCAAGCTCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: DNA nickase; Mobilization protein mbeA [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLANCFDSGTSGGDAPVRYLLGNKDHKGSERSVKPKLFRGDPESTKDLIDSINNKQKYTSGVLAFRENEVPSVKQMHQIC
DEFERYMFGGLSKDRVNILWVLHNDKGRPELHFVVPKIDLKSGKQLNISPPGKQNQDYYRLFGAYINDKFGWEQVSPKKK
WDLKAAEYKSETVPALYKRALCKQINNNYETGMFKDRNDLIKWLEQNKIQVLKQADDFITIGVGGNKLRMYGEIFKQNPD
MSVGKPYVFNQHEKNRLEHYRVERVAYFDKRFNTPPKPRFTASPQGKTTVKPQVFQTVADRIKEREVASMVQNMASIGGD
VADVMPKEQKPSSGVSSGGGSSGGADTSLQKVALSLRRNDILRQLIDATPSQVAELQNELTNIDIALGRLKLQEMEEAGR
EMDKLYELYKKPQIDEAPT

Sequences:

>Translated_419_residues
MLANCFDSGTSGGDAPVRYLLGNKDHKGSERSVKPKLFRGDPESTKDLIDSINNKQKYTSGVLAFRENEVPSVKQMHQIC
DEFERYMFGGLSKDRVNILWVLHNDKGRPELHFVVPKIDLKSGKQLNISPPGKQNQDYYRLFGAYINDKFGWEQVSPKKK
WDLKAAEYKSETVPALYKRALCKQINNNYETGMFKDRNDLIKWLEQNKIQVLKQADDFITIGVGGNKLRMYGEIFKQNPD
MSVGKPYVFNQHEKNRLEHYRVERVAYFDKRFNTPPKPRFTASPQGKTTVKPQVFQTVADRIKEREVASMVQNMASIGGD
VADVMPKEQKPSSGVSSGGGSSGGADTSLQKVALSLRRNDILRQLIDATPSQVAELQNELTNIDIALGRLKLQEMEEAGR
EMDKLYELYKKPQIDEAPT
>Mature_419_residues
MLANCFDSGTSGGDAPVRYLLGNKDHKGSERSVKPKLFRGDPESTKDLIDSINNKQKYTSGVLAFRENEVPSVKQMHQIC
DEFERYMFGGLSKDRVNILWVLHNDKGRPELHFVVPKIDLKSGKQLNISPPGKQNQDYYRLFGAYINDKFGWEQVSPKKK
WDLKAAEYKSETVPALYKRALCKQINNNYETGMFKDRNDLIKWLEQNKIQVLKQADDFITIGVGGNKLRMYGEIFKQNPD
MSVGKPYVFNQHEKNRLEHYRVERVAYFDKRFNTPPKPRFTASPQGKTTVKPQVFQTVADRIKEREVASMVQNMASIGGD
VADVMPKEQKPSSGVSSGGGSSGGADTSLQKVALSLRRNDILRQLIDATPSQVAELQNELTNIDIALGRLKLQEMEEAGR
EMDKLYELYKKPQIDEAPT

Specific function: Relaxase involved in plasmid ColE1 conjugative mobilization and is thus essential to promote the specific transfer of the plasmid during conjugation. First catalyzes the specific cleavage of one of the DNA strands at oriT, forming a covalent 5'-phosphotyr

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To E.coli mbaA and mbkA [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005094 [H]

Pfam domain/function: PF03432 Relaxase [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 47563; Mature: 47563

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANCFDSGTSGGDAPVRYLLGNKDHKGSERSVKPKLFRGDPESTKDLIDSINNKQKYTS
CCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
GVLAFRENEVPSVKQMHQICDEFERYMFGGLSKDRVNILWVLHNDKGRPELHFVVPKIDL
HHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEC
KSGKQLNISPPGKQNQDYYRLFGAYINDKFGWEQVSPKKKWDLKAAEYKSETVPALYKRA
CCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHCCCCHHHHHHHH
LCKQINNNYETGMFKDRNDLIKWLEQNKIQVLKQADDFITIGVGGNKLRMYGEIFKQNPD
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCC
MSVGKPYVFNQHEKNRLEHYRVERVAYFDKRFNTPPKPRFTASPQGKTTVKPQVFQTVAD
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RIKEREVASMVQNMASIGGDVADVMPKEQKPSSGVSSGGGSSGGADTSLQKVALSLRRND
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ILRQLIDATPSQVAELQNELTNIDIALGRLKLQEMEEAGREMDKLYELYKKPQIDEAPT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLANCFDSGTSGGDAPVRYLLGNKDHKGSERSVKPKLFRGDPESTKDLIDSINNKQKYTS
CCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
GVLAFRENEVPSVKQMHQICDEFERYMFGGLSKDRVNILWVLHNDKGRPELHFVVPKIDL
HHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEC
KSGKQLNISPPGKQNQDYYRLFGAYINDKFGWEQVSPKKKWDLKAAEYKSETVPALYKRA
CCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHCCCCHHHHHHHH
LCKQINNNYETGMFKDRNDLIKWLEQNKIQVLKQADDFITIGVGGNKLRMYGEIFKQNPD
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCC
MSVGKPYVFNQHEKNRLEHYRVERVAYFDKRFNTPPKPRFTASPQGKTTVKPQVFQTVAD
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RIKEREVASMVQNMASIGGDVADVMPKEQKPSSGVSSGGGSSGGADTSLQKVALSLRRND
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ILRQLIDATPSQVAELQNELTNIDIALGRLKLQEMEEAGREMDKLYELYKKPQIDEAPT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2671664 [H]