Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 91781855

GI number: 91781855

Start: 527601

End: 528791

Strand: Reverse

Name: tgt [H]

Synonym: Bxe_A3990

Alternate gene names: 91781855

Gene position: 528791-527601 (Counterclockwise)

Preceding gene: 91781856

Following gene: 91781854

Centisome position: 10.8

GC content: 62.64

Gene sequence:

>1191_bases
ATGACCGACGGTCATCTGTCGCAAGATGCGACCCACGAGCGTCCCGAAAACGGCCTCAAATTCGAACTGCTCGGCGTCGA
CGGCCAGGCCCGCCGCGGCCGCGTCGCCCTGAATCACGGCGTGGTGGAAACGCCGATCTTCATGCCGGTCGGCACCTACG
GCACCGTGAAAGCGGTGCAGCCGCGCGAGCTGGAGGAAATGCACGCGCAGATCATCCTCGGCAACACTTTCCATCTGTGG
CTTCGTCCGGGGCTGGAAACGATCGAAGCGTACGGCGGCCTGCATGGCTTCATGGGCTGGAAGAAGCCGATCCTGACCGA
CTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGATCTGCGCAAGATCACCGAAGATGGCGTCACGTTCGCGTCGCCGATCA
ACGGCGACAAGCTGTTCCTGTCGCCCGAAGTGTCGATGCAGATCCAGAAAGTGCTGAACTCGGACATCGTCATGCAATTC
GACGAATGCACGCCGTACGCGACCCATAACGTGCCGACCTCGCACCAGGAGGCGGCCGATTCCATGCGCATGTCGATGCG
CTGGGCACAGCGCTCGATCGACGAATTCAACCGGCTGGGCAATCCGAACGCGTTGTTCGGCATCGTTCAGGGCGGCATGT
TCGAAGACCTGCGCGACGAGTCGCTGGCCGGACTCGCGGAGAAGGATTTCCACGGTCTGGCGATCGGCGGGCTGTCGGTC
GGCGAACCGAAAGAAGACATGATGCGCGTGCTGAACCACATCGGTCCGAAGCTGCCGGCCAACAAGCCGCACTACCTGAT
GGGCGTCGGCACGCCGGAAGATCTGGTGGCGGGCGTCGCCGCCGGCGTCGACATGTTCGACTGCGTGATGCCGACCCGCA
ACGCGCGCAACGGCTGGCTCTTCACGCGTTTCGGCGACATCAAGATCCGCAACGCCGCGCACAGGAATTCGCTGCGCCCG
CTCGACGAGCAATGCGGCTGCTACACGTGCCGAAATTTCACTCGCGGCTATCTGCATCATCTGCATCGTGTTGGGGAAAT
CCTCGGTGCGCAGTTGAACACGATCCACAACCTGCACTACTACCTCGAACTGATGCAGGAAATCCGCGACGCGATCGACG
CGAAAATGTTCGAGCCGTTCCGCAAACGCTTTCACGAGAACCGCGCGCGCGGCACCGCCGAGGCGCCGTAA

Upstream 100 bases:

>100_bases
GCCGCTTCAAGGTTTTCGTCGTTCCCTCTGCCGCCGCGAACCTTCCGGCGGCGTTCAACATTTGCGCCGGACTGTTCTCC
GGTTGCACAGGAGTTATTTC

Downstream 100 bases:

>100_bases
CAGCGCGGGGTAACGGATTCAGCCGAAGAATCCGTCGGCCTCGCGAAGAAAACCTTACAATTAACTTTCGCGGACGGCGG
TAAAGACGGCGAAGCAGGCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTDGHLSQDATHERPENGLKFELLGVDGQARRGRVALNHGVVETPIFMPVGTYGTVKAVQPRELEEMHAQIILGNTFHLW
LRPGLETIEAYGGLHGFMGWKKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQF
DECTPYATHNVPTSHQEAADSMRMSMRWAQRSIDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAEKDFHGLAIGGLSV
GEPKEDMMRVLNHIGPKLPANKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDIKIRNAAHRNSLRP
LDEQCGCYTCRNFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMQEIRDAIDAKMFEPFRKRFHENRARGTAEAP

Sequences:

>Translated_396_residues
MTDGHLSQDATHERPENGLKFELLGVDGQARRGRVALNHGVVETPIFMPVGTYGTVKAVQPRELEEMHAQIILGNTFHLW
LRPGLETIEAYGGLHGFMGWKKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQF
DECTPYATHNVPTSHQEAADSMRMSMRWAQRSIDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAEKDFHGLAIGGLSV
GEPKEDMMRVLNHIGPKLPANKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDIKIRNAAHRNSLRP
LDEQCGCYTCRNFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMQEIRDAIDAKMFEPFRKRFHENRARGTAEAP
>Mature_395_residues
TDGHLSQDATHERPENGLKFELLGVDGQARRGRVALNHGVVETPIFMPVGTYGTVKAVQPRELEEMHAQIILGNTFHLWL
RPGLETIEAYGGLHGFMGWKKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFD
ECTPYATHNVPTSHQEAADSMRMSMRWAQRSIDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAEKDFHGLAIGGLSVG
EPKEDMMRVLNHIGPKLPANKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDIKIRNAAHRNSLRPL
DEQCGCYTCRNFTRGYLHHLHRVGEILGAQLNTIHNLHYYLELMQEIRDAIDAKMFEPFRKRFHENRARGTAEAP

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=43.5393258426966, Blast_Score=298, Evalue=6e-81,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=62.9032258064516, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=42.6630434782609, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=28.0487804878049, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=44.2577030812325, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21355311, Length=223, Percent_Identity=25.1121076233184, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44305; Mature: 44174

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGHLSQDATHERPENGLKFELLGVDGQARRGRVALNHGVVETPIFMPVGTYGTVKAVQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEECCEEEECCCCCCEECCC
PRELEEMHAQIILGNTFHLWLRPGLETIEAYGGLHGFMGWKKPILTDSGGFQVFSLGDLR
CHHHHHHHHEEEECCEEEEEECCCHHHHHHHCCHHHHCCCCCCCEECCCCEEEEECCHHH
KITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATHNVPTSHQEAAD
HHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHH
SMRMSMRWAQRSIDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAEKDFHGLAIGGLSV
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCHHCCCCCEEECCCCC
GEPKEDMMRVLNHIGPKLPANKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWL
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEE
FTRFGDIKIRNAAHRNSLRPLDEQCGCYTCRNFTRGYLHHLHRVGEILGAQLNTIHNLHY
EEEECCEEEECCHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLELMQEIRDAIDAKMFEPFRKRFHENRARGTAEAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDGHLSQDATHERPENGLKFELLGVDGQARRGRVALNHGVVETPIFMPVGTYGTVKAVQ
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCEECCEEEECCCCCCEECCC
PRELEEMHAQIILGNTFHLWLRPGLETIEAYGGLHGFMGWKKPILTDSGGFQVFSLGDLR
CHHHHHHHHEEEECCEEEEEECCCHHHHHHHCCHHHHCCCCCCCEECCCCEEEEECCHHH
KITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATHNVPTSHQEAAD
HHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHH
SMRMSMRWAQRSIDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAEKDFHGLAIGGLSV
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCHHCCCCCEEECCCCC
GEPKEDMMRVLNHIGPKLPANKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWL
CCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEE
FTRFGDIKIRNAAHRNSLRPLDEQCGCYTCRNFTRGYLHHLHRVGEILGAQLNTIHNLHY
EEEECCEEEECCHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLELMQEIRDAIDAKMFEPFRKRFHENRARGTAEAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA