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The map label for this gene is queF [H]

Identifier: 91781789

GI number: 91781789

Start: 449005

End: 449829

Strand: Reverse

Name: queF [H]

Synonym: Bxe_A4056

Alternate gene names: 91781789

Gene position: 449829-449005 (Counterclockwise)

Preceding gene: 91781790

Following gene: 91781785

Centisome position: 9.19

GC content: 59.15

Gene sequence:

>825_bases
ATGACACCCGAACAATCCCCGCTGGGTAAGGCCTCCACTTACACCGAACAATACGACGCGTCGCTGCTGTTTCCGATCGC
GCGAAAAAATGCGCGCGAGGCGATCGGCATCGGCGCGCAGTTGCCGTTCTTCGGCACGGACATCTGGAATGCCTACGAAC
TGTCCTGGCTGAATGCGCGCGGCAAGCCGCAAATCGCGGTGGCGACTTTCTTCGTGCCGGCGGATTCGCCGAATATCGTC
GAATCCAAGTCGTTCAAGCTTTATCTCGGCTCGTTCGCGCAGACCGCTTTCGAGTCGATGGACGCCGTACGCGACACGAT
CAGGCGCGATGTGTCCGCGTCGTGCGGCGCGACGGTTTCCGTTCATCTAACGGCGCCGTACAAGTTCGGCAAATTGCAGA
TGGAAGAATTCGAAGGCTTGTCGCTGGACCGGCTGGATCTGGACACCGACGTTTATCAGCCGGACGCGTCTTTGCTGAAG
GCGGCACTGCATGAAGCGCCGGTCGAAGAAACGGTGTTTTCCAACTTGCTGAAGTCGAATTGCCCGGTAACGGGGCAGCC
GGATTGGGGCAGCGTGCAGATTCATTACGTGGGCCCGCAGATCGATCACGCAGGCTTGCTGCGCTACATCATCTCGTACC
GGAATCACACCGGCTTTCACGAGCAATGTGTCGAGCGGATTTTCGTCGACGTGCTGAAGGCCTGCAAGCCGGTGAAGCTT
GCGGTGTACGCGCGTTATACGCGGCGCGGCGGGCTGGACATCAATCCGTTCCGCACGAATTACAACTTGCCGATGCCGGA
CAATATGCGGCTCGCGCGGCAGTAA

Upstream 100 bases:

>100_bases
TCGAGCCGGACTTTTTCTTCGACGACCAAATTGGCCACTGCGAATCGGCCCGCGTCGTGACGGCGACGGGGCATGTGATG
AGTGGCATCGTGAACGCATC

Downstream 100 bases:

>100_bases
GAAAAGCAGGTTGTTCGCCGCGCCGAGTCGATGCGAATTTGCGGCGAACGAACCGGCTGAATGTGCAGGCTTAGGCCGTG
GGCTTCTTATAAGCCACGCA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTPEQSPLGKASTYTEQYDASLLFPIARKNAREAIGIGAQLPFFGTDIWNAYELSWLNARGKPQIAVATFFVPADSPNIV
ESKSFKLYLGSFAQTAFESMDAVRDTIRRDVSASCGATVSVHLTAPYKFGKLQMEEFEGLSLDRLDLDTDVYQPDASLLK
AALHEAPVEETVFSNLLKSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISYRNHTGFHEQCVERIFVDVLKACKPVKL
AVYARYTRRGGLDINPFRTNYNLPMPDNMRLARQ

Sequences:

>Translated_274_residues
MTPEQSPLGKASTYTEQYDASLLFPIARKNAREAIGIGAQLPFFGTDIWNAYELSWLNARGKPQIAVATFFVPADSPNIV
ESKSFKLYLGSFAQTAFESMDAVRDTIRRDVSASCGATVSVHLTAPYKFGKLQMEEFEGLSLDRLDLDTDVYQPDASLLK
AALHEAPVEETVFSNLLKSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISYRNHTGFHEQCVERIFVDVLKACKPVKL
AVYARYTRRGGLDINPFRTNYNLPMPDNMRLARQ
>Mature_273_residues
TPEQSPLGKASTYTEQYDASLLFPIARKNAREAIGIGAQLPFFGTDIWNAYELSWLNARGKPQIAVATFFVPADSPNIVE
SKSFKLYLGSFAQTAFESMDAVRDTIRRDVSASCGATVSVHLTAPYKFGKLQMEEFEGLSLDRLDLDTDVYQPDASLLKA
ALHEAPVEETVFSNLLKSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISYRNHTGFHEQCVERIFVDVLKACKPVKLA
VYARYTRRGGLDINPFRTNYNLPMPDNMRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=272, Percent_Identity=52.2058823529412, Blast_Score=274, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30659; Mature: 30528

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQSPLGKASTYTEQYDASLLFPIARKNAREAIGIGAQLPFFGTDIWNAYELSWLNAR
CCCCCCCCCCCHHHHHHHCCEEEEHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCC
GKPQIAVATFFVPADSPNIVESKSFKLYLGSFAQTAFESMDAVRDTIRRDVSASCGATVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VHLTAPYKFGKLQMEEFEGLSLDRLDLDTDVYQPDASLLKAALHEAPVEETVFSNLLKSN
EEEECCCCCCCEEHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISYRNHTGFHEQCVERIFVDVLKACKPVKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNLPMPDNMRLARQ
EEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
TPEQSPLGKASTYTEQYDASLLFPIARKNAREAIGIGAQLPFFGTDIWNAYELSWLNAR
CCCCCCCCCCHHHHHHHCCEEEEHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCC
GKPQIAVATFFVPADSPNIVESKSFKLYLGSFAQTAFESMDAVRDTIRRDVSASCGATVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VHLTAPYKFGKLQMEEFEGLSLDRLDLDTDVYQPDASLLKAALHEAPVEETVFSNLLKSN
EEEECCCCCCCEEHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISYRNHTGFHEQCVERIFVDVLKACKPVKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNLPMPDNMRLARQ
EEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA