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The map label for this gene is yxeQ [H]

Identifier: 91781759

GI number: 91781759

Start: 414863

End: 416275

Strand: Direct

Name: yxeQ [H]

Synonym: Bxe_A4086

Alternate gene names: 91781759

Gene position: 414863-416275 (Clockwise)

Preceding gene: 91781758

Following gene: 91781760

Centisome position: 8.47

GC content: 67.3

Gene sequence:

>1413_bases
ATGACTTCACTCGACTCCTCCGTCACGCTCTCTGGTCAGTACGCGCGCTTCGGCGTGGGCCTGCAATTGCGCGACGTTCC
GCATGCGGTGCAGACGCGCGCCAGGCATCTGATTCTCGACGCCGTAGGCATTGCGCTCGCGTCGCGCGGCTATCCGTATG
CGGATGTCTCGTTCGCCGCATTCTCCGAGTTCGGCGGCGGCACCTCGCCGGTGATCGGCTACGGCCGGCGGCTCGGCCTG
CGCGATGCCGCGATGATGAACGGCGTGCTGATTCACGGTCTCGACTTCGACGACACGCATTCGCGCGGCGTCATCCATTC
GACCGCCAGCGCCCTGTCTTGCGTGCTCGCGCTCGCCGATCGCGACGCCGTGAGCGGCGCGGATCTGCTGGCCGCATATA
TCGTCGCGATGGAAGTGTCGACGCGCGTGGCGTCGGCGGCCAGGGGCGGATTTCACGACGCCGGTTTTCATCCGACAGGC
CTGGTCGGCGCGTTCGGCTGCGCGGTTGCCGCGGCGCGTCTGTTGCAACTGGACGAGGCGCGGGCCGCGCATGCGCAAGG
CATCGTGCTGTCGATGGCGGCCGGCAGCCTCGAATTCCTCGAAGACGGCGCGTGGACCAAACGCGCCCATCCCGGCTTCG
CGGCCGCTTCGGCCATTACGGCAGCGACGCTCGCGAAGCACGGCTTCACCGGCCCGCGTCTGGCCTACGAAGGACGCTTC
GGTCTCTACGCGAGCCACCTCGGCAAGCCGCTCGACGCGGCCGATCGCGAACTCGCCAGCGAAGGCCTCGGCAGCTCATG
GCAGATCGATGAAGTAGCGATCAAACCGCTGCCCGCTTGCCACTTCACGCATGCGGTGGCCGATGCGGCGATGGCGCTGC
ATCGCGAGCATCGTTTTTCCGCTGAATCGCTCGACTCGATCCGGCGTGTGGTTGCCAGGGTGCCGCGCGGTACGGTCGAG
ATCGTGTGCGAACCGGTCGATGCGAAGCGCAAACCGGTTACCGCCTACGACGCGCAATTCAGCGTGCCGTACATCGTCGC
CACCGCGTTGCTCAAAGGCCGCTTCACGCTCGACGAACTCGAACCGGCCGCGCTCGCCGATCCCGCCGTGCTGGCGCTTG
CTGCGCGGGTCGATTACGAAATCGACCCCGACACGACTTTCCCGCGCCACTACACCGGCGAAGTCGTGGTGGAGCGCAGC
GACGGTTCGCGCGTCGCGCATCGCGAGGCGATCAATCGCGGTTGCGCGGACCGGCCCGTCAGCAACGACGACATCGTCGC
CAAGTTCCACGCGAACGCGCAGCGCAGCGTTTCGCGCAGCGCCGCCGAACAGATCGCCCAAGCCGTATTGCAACTCGACG
AACGGCCGGCCCGCGCGCTTGCCGACACGCTTGCTGGACTCACCGCATCCTGA

Upstream 100 bases:

>100_bases
GCTGCGTGTGGAAACCAATCAGCACGCGCTCGAGCGGATCTCGCCGAGCCTCGACACCGATTCCGATCCGATGCCCGCTA
CCCAGCAAGGTTAAATCACC

Downstream 100 bases:

>100_bases
GGACGTCATCATGACCGCAACTGAACTCGACACGCTGGAAAACGATCAACTGATTCTCGACATGCTGGACCGCTTTCTCG
AAACCGAGGTGCGTCCTTAC

Product: hypothetical protein

Products: cis-2-Methylaconitate; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTSLDSSVTLSGQYARFGVGLQLRDVPHAVQTRARHLILDAVGIALASRGYPYADVSFAAFSEFGGGTSPVIGYGRRLGL
RDAAMMNGVLIHGLDFDDTHSRGVIHSTASALSCVLALADRDAVSGADLLAAYIVAMEVSTRVASAARGGFHDAGFHPTG
LVGAFGCAVAAARLLQLDEARAAHAQGIVLSMAAGSLEFLEDGAWTKRAHPGFAAASAITAATLAKHGFTGPRLAYEGRF
GLYASHLGKPLDAADRELASEGLGSSWQIDEVAIKPLPACHFTHAVADAAMALHREHRFSAESLDSIRRVVARVPRGTVE
IVCEPVDAKRKPVTAYDAQFSVPYIVATALLKGRFTLDELEPAALADPAVLALAARVDYEIDPDTTFPRHYTGEVVVERS
DGSRVAHREAINRGCADRPVSNDDIVAKFHANAQRSVSRSAAEQIAQAVLQLDERPARALADTLAGLTAS

Sequences:

>Translated_470_residues
MTSLDSSVTLSGQYARFGVGLQLRDVPHAVQTRARHLILDAVGIALASRGYPYADVSFAAFSEFGGGTSPVIGYGRRLGL
RDAAMMNGVLIHGLDFDDTHSRGVIHSTASALSCVLALADRDAVSGADLLAAYIVAMEVSTRVASAARGGFHDAGFHPTG
LVGAFGCAVAAARLLQLDEARAAHAQGIVLSMAAGSLEFLEDGAWTKRAHPGFAAASAITAATLAKHGFTGPRLAYEGRF
GLYASHLGKPLDAADRELASEGLGSSWQIDEVAIKPLPACHFTHAVADAAMALHREHRFSAESLDSIRRVVARVPRGTVE
IVCEPVDAKRKPVTAYDAQFSVPYIVATALLKGRFTLDELEPAALADPAVLALAARVDYEIDPDTTFPRHYTGEVVVERS
DGSRVAHREAINRGCADRPVSNDDIVAKFHANAQRSVSRSAAEQIAQAVLQLDERPARALADTLAGLTAS
>Mature_469_residues
TSLDSSVTLSGQYARFGVGLQLRDVPHAVQTRARHLILDAVGIALASRGYPYADVSFAAFSEFGGGTSPVIGYGRRLGLR
DAAMMNGVLIHGLDFDDTHSRGVIHSTASALSCVLALADRDAVSGADLLAAYIVAMEVSTRVASAARGGFHDAGFHPTGL
VGAFGCAVAAARLLQLDEARAAHAQGIVLSMAAGSLEFLEDGAWTKRAHPGFAAASAITAATLAKHGFTGPRLAYEGRFG
LYASHLGKPLDAADRELASEGLGSSWQIDEVAIKPLPACHFTHAVADAAMALHREHRFSAESLDSIRRVVARVPRGTVEI
VCEPVDAKRKPVTAYDAQFSVPYIVATALLKGRFTLDELEPAALADPAVLALAARVDYEIDPDTTFPRHYTGEVVVERSD
GSRVAHREAINRGCADRPVSNDDIVAKFHANAQRSVSRSAAEQIAQAVLQLDERPARALADTLAGLTAS

Specific function: Catalyzes The Dehydration Of 2-Methylcitrate To 2- Methyl-Cis-Aconitate. Also Seems To Be Responsible For The Residual Aconitase Activity Of The Acnab-Null Strain. [C]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Homo sapiens, GI310129732, Length=453, Percent_Identity=31.1258278145695, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI310110533, Length=453, Percent_Identity=31.1258278145695, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI310123058, Length=453, Percent_Identity=31.1258278145695, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: 4.2.1.79 [C]

Molecular weight: Translated: 49606; Mature: 49475

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLDSSVTLSGQYARFGVGLQLRDVPHAVQTRARHLILDAVGIALASRGYPYADVSFAA
CCCCCCCEEECCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FSEFGGGTSPVIGYGRRLGLRDAAMMNGVLIHGLDFDDTHSRGVIHSTASALSCVLALAD
HHHCCCCCCCHHHCCHHCCCHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
RDAVSGADLLAAYIVAMEVSTRVASAARGGFHDAGFHPTGLVGAFGCAVAAARLLQLDEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAAHAQGIVLSMAAGSLEFLEDGAWTKRAHPGFAAASAITAATLAKHGFTGPRLAYEGRF
HHHHHCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCC
GLYASHLGKPLDAADRELASEGLGSSWQIDEVAIKPLPACHFTHAVADAAMALHREHRFS
CHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
AESLDSIRRVVARVPRGTVEIVCEPVDAKRKPVTAYDAQFSVPYIVATALLKGRFTLDEL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHC
EPAALADPAVLALAARVDYEIDPDTTFPRHYTGEVVVERSDGSRVAHREAINRGCADRPV
CCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCC
SNDDIVAKFHANAQRSVSRSAAEQIAQAVLQLDERPARALADTLAGLTAS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSLDSSVTLSGQYARFGVGLQLRDVPHAVQTRARHLILDAVGIALASRGYPYADVSFAA
CCCCCCEEECCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
FSEFGGGTSPVIGYGRRLGLRDAAMMNGVLIHGLDFDDTHSRGVIHSTASALSCVLALAD
HHHCCCCCCCHHHCCHHCCCHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHC
RDAVSGADLLAAYIVAMEVSTRVASAARGGFHDAGFHPTGLVGAFGCAVAAARLLQLDEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAAHAQGIVLSMAAGSLEFLEDGAWTKRAHPGFAAASAITAATLAKHGFTGPRLAYEGRF
HHHHHCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCC
GLYASHLGKPLDAADRELASEGLGSSWQIDEVAIKPLPACHFTHAVADAAMALHREHRFS
CHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
AESLDSIRRVVARVPRGTVEIVCEPVDAKRKPVTAYDAQFSVPYIVATALLKGRFTLDEL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHC
EPAALADPAVLALAARVDYEIDPDTTFPRHYTGEVVVERSDGSRVAHREAINRGCADRPV
CCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCC
SNDDIVAKFHANAQRSVSRSAAEQIAQAVLQLDERPARALADTLAGLTAS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Methylcitrate [C]

Specific reaction: 2-Methylcitrate --> cis-2-Methylaconitate + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]