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The map label for this gene is phbI [H]

Identifier: 91781662

GI number: 91781662

Start: 313061

End: 314866

Strand: Direct

Name: phbI [H]

Synonym: Bxe_A4184

Alternate gene names: 91781662

Gene position: 313061-314866 (Clockwise)

Preceding gene: 91781661

Following gene: 91781666

Centisome position: 6.39

GC content: 63.84

Gene sequence:

>1806_bases
GTGTCTGAGAGCATTGCAGCGGTTTGCCGCGGCATTACACAACTAGAGGAGGTGCGCGTGTCCTTCACGCTGCATGGAAT
TCCCGTGTCACGCGGTATCGCCATCGGGCGGGCCTATCTGATCGCCCCGGCTGCACTTGACGTAGACCACTATCTGATTG
AACCGGCCCAGATCGACGGCGAGGTCGAGCGCTTTCGCGCGGCCCAGCAACACGTGCATCGCGAGCTCGACGCGTTGCGC
GCCGATCTCGCCGCAGACGCACCGAGCGAGATGGGCGCGTTCATCAACGTCCACTCAATGATCCTGAACGACGCGATGCT
CGTGCAGGAAACCATCGACCTGATCCGCACGCGCCGCTACAACGTCGAGTGGGCGCTGACCGAACAGCTCGAGCGCCTGT
CGCGCCATTTCGACGATATCGAAGACGAATACCTGCGCGAGCGCAAAGCCGATATCGAGCAGGTGGTGGAGCGCGTGCTG
AAGGCGCTCGCCGGCGCGTCGGTCGCGCTGGTGGACGGCGTGCACGGCGCCTGCGACGAGATGATCGTGGTCGCGCACGA
CATCGCGCCGGCCGACATGATGCAATTCAAAACGCAAACGTTCCAGGGTTTCGTCACGGACCTCGGCGGCCGCACCTCGC
ATACAGCGATCGTCGCGCGCAGCCTGGGCATTCCGGCCGCGGTCGGCGTGCAGCATGCGAGCGCGTTGATTCGCCAGGAC
GACCTGATCATTGTCGACGGCGATCATGGCATCGTGATCGTCGATCCCGCGCCGATCGTGCTGGAGGAGTACTCGTACCG
GCAGAGCGAGAAAGCGCTCGAACAGCGCAAGCTGCAGCGGTTGAAGTTTTCGCCCACCCAAACGCTGTGCGGCACGCGTA
TCGAGTTGTGCGCGAACATCGAGTTGCCGGAAGACGCGCGCGCGGCGCTCGAGGCGGGGGCGATGGGTGTCGGCCTGTTC
CGCTCCGAGTTCCTGTTCATGAATCACAAGGACCGCTTGCCGGAAGAGGAAGAGCAATTCGCCGCCTACCGTCGCGCGGT
CGAATTGATGAACGGCCTGCCGGTCACGATCCGCACGATCGACGTGGGCGCCGACAAGCCGCTCGATTCGATGGGCGGGG
GCGACGGCTATGAAACCGCCGCGAACCCGGCCCTGGGTCTGAGAGCGATTCGCTGGAGTCTCTCCGAGCCGCAGATGTTC
CTCACGCAGTTGCGCGCAATACTGCGCGCGTCGGCGTTCGGTACCGTGAAGATTCTGATTCCGATGCTCGCGCATGCGCA
GGAGATCGACCAGACACTCGATCTGATCCGCGAAGCCAAGCGTCAGCTCGACGACGCGGGCATCGCCTACGATCCGAACG
TGCAGGTGGGCGCGATGATCGAGATTCCGGCTGCCGCCATCGCATTGCCGCTGTTCCTGAAGCGGCTCGATTTTCTGTCG
ATCGGCACCAACGATCTGATCCAGTACACGCTGGCGATCGATCGCGCGGACAACGCGGTCGCGCATCTATATGACCCGCT
GCATCCCGCGGTGCTGCATCTGATCGCGTTCACGCTGCGCGAGGCGAAGCGGGCGGGTGTGCCGGTGTCGGTGTGCGGCG
AAATGGCCGGGGACCCGGCGTTGACGCGTCTGTTGCTCGGCATGGGTCTCACCGAGTTCTCGATGCATCCGAGCCAGCTG
CTGGTGGTCAAGCAGGAAGTGCTGCGCGCGCATCTGAAGACGCTGGAAAAGCCGGTGGCCGATGTGCTGGCTTCGTTCGA
ACCGGAAGAAGTGCAGGCCGCACTGAAGCGGGTGACGCTGGCGTGA

Upstream 100 bases:

>100_bases
GCAGACGAAAGCGTGCGAGGGCGCGCTTACGGCAATTCGTGCTGCGCTGCACACAGCCCCGGCGTATCTGCGAGGGGTGC
GGAATTTATAATGGCGAAGA

Downstream 100 bases:

>100_bases
GTTGCCGGATTCACGTCTTCGAATGAGAAACGCCGCCTTATGGGCGGCATTTCTTTTTCAGCCTTGATTGCATCGACAGC
CTCGATGCACGAAATCAGAC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSESIAAVCRGITQLEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIDGEVERFRAAQQHVHRELDALR
ADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVL
KALAGASVALVDGVHGACDEMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQD
DLIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANIELPEDARAALEAGAMGVGLF
RSEFLFMNHKDRLPEEEEQFAAYRRAVELMNGLPVTIRTIDVGADKPLDSMGGGDGYETAANPALGLRAIRWSLSEPQMF
LTQLRAILRASAFGTVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGIAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLS
IGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQL
LVVKQEVLRAHLKTLEKPVADVLASFEPEEVQAALKRVTLA

Sequences:

>Translated_601_residues
MSESIAAVCRGITQLEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIDGEVERFRAAQQHVHRELDALR
ADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVL
KALAGASVALVDGVHGACDEMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQD
DLIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANIELPEDARAALEAGAMGVGLF
RSEFLFMNHKDRLPEEEEQFAAYRRAVELMNGLPVTIRTIDVGADKPLDSMGGGDGYETAANPALGLRAIRWSLSEPQMF
LTQLRAILRASAFGTVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGIAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLS
IGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQL
LVVKQEVLRAHLKTLEKPVADVLASFEPEEVQAALKRVTLA
>Mature_600_residues
SESIAAVCRGITQLEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIDGEVERFRAAQQHVHRELDALRA
DLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVLK
ALAGASVALVDGVHGACDEMIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDD
LIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANIELPEDARAALEAGAMGVGLFR
SEFLFMNHKDRLPEEEEQFAAYRRAVELMNGLPVTIRTIDVGADKPLDSMGGGDGYETAANPALGLRAIRWSLSEPQMFL
TQLRAILRASAFGTVKILIPMLAHAQEIDQTLDLIREAKRQLDDAGIAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLSI
GTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLL
VVKQEVLRAHLKTLEKPVADVLASFEPEEVQAALKRVTLA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=542, Percent_Identity=39.6678966789668, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=34.8230912476723, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI48994992, Length=471, Percent_Identity=33.5456475583864, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1788726, Length=549, Percent_Identity=28.0510018214936, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1787994, Length=410, Percent_Identity=27.8048780487805, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI226510935, Length=167, Percent_Identity=26.3473053892216, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 66025; Mature: 65894

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESIAAVCRGITQLEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIDG
CCCHHHHHHHHHHHHHHHEEEEEEECCCHHCCHHHHHHHEEECHHCCCHHHEECCHHCCC
EVERFRAAQQHVHRELDALRADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVLKALAGASVALVDGVHGACDE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
MIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQD
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
DLIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANI
CEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHEECC
ELPEDARAALEAGAMGVGLFRSEFLFMNHKDRLPEEEEQFAAYRRAVELMNGLPVTIRTI
CCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
DVGADKPLDSMGGGDGYETAANPALGLRAIRWSLSEPQMFLTQLRAILRASAFGTVKILI
ECCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
PMLAHAQEIDQTLDLIREAKRQLDDAGIAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHC
IGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPA
CCCHHHHEEHHEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHH
LTRLLLGMGLTEFSMHPSQLLVVKQEVLRAHLKTLEKPVADVLASFEPEEVQAALKRVTL
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
A
C
>Mature Secondary Structure 
SESIAAVCRGITQLEEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIDG
CCHHHHHHHHHHHHHHHEEEEEEECCCHHCCHHHHHHHEEECHHCCCHHHEECCHHCCC
EVERFRAAQQHVHRELDALRADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVEWALTEQLERLSRHFDDIEDEYLRERKADIEQVVERVLKALAGASVALVDGVHGACDE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
MIVVAHDIAPADMMQFKTQTFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQD
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
DLIIVDGDHGIVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANI
CEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHEECC
ELPEDARAALEAGAMGVGLFRSEFLFMNHKDRLPEEEEQFAAYRRAVELMNGLPVTIRTI
CCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
DVGADKPLDSMGGGDGYETAANPALGLRAIRWSLSEPQMFLTQLRAILRASAFGTVKILI
ECCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
PMLAHAQEIDQTLDLIREAKRQLDDAGIAYDPNVQVGAMIEIPAAAIALPLFLKRLDFLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHC
IGTNDLIQYTLAIDRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPA
CCCHHHHEEHHEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHH
LTRLLLGMGLTEFSMHPSQLLVVKQEVLRAHLKTLEKPVADVLASFEPEEVQAALKRVTL
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]