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The map label for this gene is gcvA [H]

Identifier: 91781631

GI number: 91781631

Start: 281575

End: 282543

Strand: Reverse

Name: gcvA [H]

Synonym: Bxe_A4215

Alternate gene names: 91781631

Gene position: 282543-281575 (Counterclockwise)

Preceding gene: 91781636

Following gene: 91781620

Centisome position: 5.77

GC content: 67.8

Gene sequence:

>969_bases
ATGGACCTCCGGCAACTACCCGCGTTGAACGCGATCAAGGCATTCGAGGCCGCCGCCCGTCACGAAAGCTTCTCGCGCGC
CGCCGACGAACTCTTCGTCACCCACGGCGCCGTCAGCCACCAGATCCGAGCGCTGGAGGCCGAGCTGGGCGTTTCGCTGT
TCGCGCGCGACGGCAAGCGCGTGCGGCTCACCGAAACCGGCCGGCGTTACGCCACGCACGTGCGCGCCGCGCTGATGGCG
CTCGCCGACGCCACCCGCCAGATCCGCGCGGGCGACCGCGAACGGCGGCTCGTGGTGTCCATGCTGTCGTCGTTTGCGGC
GCGCTGGGTGACGCCGCGCATCGGCAGCTTTATCGAGGCGCATCCGCAGTGGGACCTCGAACTCCTGTCCACCAACGCGC
TAACGGATTTCGCGCGCGACGACGTCGACGTGGCGATCCGTTTCGGCTTAGGCAAATATTCGGGGCTGCATGCGGAGTTG
CTGCTGGAGGAGATCTTCTTTCCCGCCTGCGCGCCGGATTTCAACGGCGGCAAGCTGCCGCAAACGCCGGCCGATCTGGC
CAACGTCCCGCTGCTGCGTTCCGACGACGAACTCTGGCGGCCTTGGTTCGACGCCGCCGGCCTCTCCGACTGGCCCGAAC
CAAAGCGCGGCGTGCTGTACCAGGATTCGTCGAATCTGCTGCAAGCGGCCATCGACGGCCAGGGCGTCGCGCTCACGCGC
CGCTCGCTGGCCATGCACGAAATCGCGGCCGGCCGCCTCGTGCGCTTATTCGACGTGGATGGGCCGAGCCCGTGGCAGTA
CTACTTCATCTGTCCGCCGCAGATGCTCGAAACCGCCCGGGTCAGGGCGTTCCGCGACTGGGTGTTCGAGGAGGTGGGAC
GCTTCAGGCAACTGTTCGACCGGGCCTGCGAGGTGGGACCCGCCGCAAGCGCCGATATGGCGGCGAACGCATTGCGCGTC
ACGCCATAA

Upstream 100 bases:

>100_bases
ATGATACCCTCGCCAAAAATCGTTTCGTGACTACAATCCTAGGCGTAGCAAGGGTTTACGCAAAACGATCTATTTTCACC
TCTATGTGAGAAATTTTGTC

Downstream 100 bases:

>100_bases
AACAAGGCGCCGGGCGAGGCTTTACAGCACCACCTTCACCATCGGGTGGCCGGTGAGCGCGCGGTAGATATCGTCCAACT
GCTCGCGGCTCGTGGCGCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDLRQLPALNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRALEAELGVSLFARDGKRVRLTETGRRYATHVRAALMA
LADATRQIRAGDRERRLVVSMLSSFAARWVTPRIGSFIEAHPQWDLELLSTNALTDFARDDVDVAIRFGLGKYSGLHAEL
LLEEIFFPACAPDFNGGKLPQTPADLANVPLLRSDDELWRPWFDAAGLSDWPEPKRGVLYQDSSNLLQAAIDGQGVALTR
RSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQMLETARVRAFRDWVFEEVGRFRQLFDRACEVGPAASADMAANALRV
TP

Sequences:

>Translated_322_residues
MDLRQLPALNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRALEAELGVSLFARDGKRVRLTETGRRYATHVRAALMA
LADATRQIRAGDRERRLVVSMLSSFAARWVTPRIGSFIEAHPQWDLELLSTNALTDFARDDVDVAIRFGLGKYSGLHAEL
LLEEIFFPACAPDFNGGKLPQTPADLANVPLLRSDDELWRPWFDAAGLSDWPEPKRGVLYQDSSNLLQAAIDGQGVALTR
RSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQMLETARVRAFRDWVFEEVGRFRQLFDRACEVGPAASADMAANALRV
TP
>Mature_322_residues
MDLRQLPALNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRALEAELGVSLFARDGKRVRLTETGRRYATHVRAALMA
LADATRQIRAGDRERRLVVSMLSSFAARWVTPRIGSFIEAHPQWDLELLSTNALTDFARDDVDVAIRFGLGKYSGLHAEL
LLEEIFFPACAPDFNGGKLPQTPADLANVPLLRSDDELWRPWFDAAGLSDWPEPKRGVLYQDSSNLLQAAIDGQGVALTR
RSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQMLETARVRAFRDWVFEEVGRFRQLFDRACEVGPAASADMAANALRV
TP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=301, Percent_Identity=40.8637873754153, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786448, Length=303, Percent_Identity=32.6732673267327, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=29.1525423728814, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=30.0675675675676, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=28.9198606271777, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=26.1324041811847, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790783, Length=262, Percent_Identity=27.4809160305344, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=209, Percent_Identity=25.8373205741627, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35903; Mature: 35903

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLPALNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRALEAELGVSLFARDGKR
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHEEECCCCE
VRLTETGRRYATHVRAALMALADATRQIRAGDRERRLVVSMLSSFAARWVTPRIGSFIEA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHC
HPQWDLELLSTNALTDFARDDVDVAIRFGLGKYSGLHAELLLEEIFFPACAPDFNGGKLP
CCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
QTPADLANVPLLRSDDELWRPWFDAAGLSDWPEPKRGVLYQDSSNLLQAAIDGQGVALTR
CCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCEEHHH
RSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQMLETARVRAFRDWVFEEVGRFRQLFD
HHHHHHHHHHCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACEVGPAASADMAANALRVTP
HHHCCCCCCCHHHHCCCEECCC
>Mature Secondary Structure
MDLRQLPALNAIKAFEAAARHESFSRAADELFVTHGAVSHQIRALEAELGVSLFARDGKR
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHEEECCCCE
VRLTETGRRYATHVRAALMALADATRQIRAGDRERRLVVSMLSSFAARWVTPRIGSFIEA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHC
HPQWDLELLSTNALTDFARDDVDVAIRFGLGKYSGLHAELLLEEIFFPACAPDFNGGKLP
CCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
QTPADLANVPLLRSDDELWRPWFDAAGLSDWPEPKRGVLYQDSSNLLQAAIDGQGVALTR
CCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCEEHHH
RSLAMHEIAAGRLVRLFDVDGPSPWQYYFICPPQMLETARVRAFRDWVFEEVGRFRQLFD
HHHHHHHHHHCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACEVGPAASADMAANALRVTP
HHHCCCCCCCHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]