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The map label for this gene is sbp [H]

Identifier: 91781579

GI number: 91781579

Start: 227809

End: 228834

Strand: Direct

Name: sbp [H]

Synonym: Bxe_A4267

Alternate gene names: 91781579

Gene position: 227809-228834 (Clockwise)

Preceding gene: 91781578

Following gene: 91781584

Centisome position: 4.65

GC content: 63.06

Gene sequence:

>1026_bases
ATGGCAGGCAAGACGGGGACTTCGCGCTGGCTGGCAGCCGGCATGACGGCAATGACGCTCGCGCTGGCGGGCGTGCCGGC
AGCGCACGCAGAGACGTCGCTGCTCAACGTGTCGTACGACGTGACGCGTGAGCTGTACAAGGACATCAACGCTGGCTTCA
TTGCCGCGTACAAGCAGAAGAGCGGCGAGACCGTCTCGATCCGCCAGTCGCATGGCGCGTCGAGCGCGCAGGCGCTGTCG
GTGCTGCAAGGCTTGCAGGCCGACGTGGTGACGATGAACCAGCCGAACGACATCGACCTGCTCGCCGAGAAAGGTCAACT
GCTGCCGGCCAACTGGCGCACGCGTCTGCCGAACGACAGCGTGCCCTACACCACCACGATGGTGTTCCTCGTGCGCAAGG
GCAACCCGAAGCACATCAAGGACTGGGACGATCTCGCGAGGCCAGGCGTGCAGGTGATCATCGCCAATCCGAAGACATCG
GGTAACGGGCGCTATACGTATCTGGCCGCGTGGGGTTATCGCAAGCAGCACGGCGGGACCGACGCGCAGGCGCTCGACTT
CGAAAAGGCGATCTTCAAAAACGTGCCGGTGCTCGACACGGGCGGCCGCGGCGCGACGACCACGTTTACGCAACGCGGTA
TCGGCGACGTGCTGGTGACGTTCGAAAACGAAGTGGCGCTGATGGACACGGGCGTGGGCGGGGGCAACTTCGAGGCGGTG
TATCCGTCCGTGAGTTTGCTGGCGGCGCCGCCGGTTTCGATCGTCGACAAGGTGGTCGACAAGCGCGGCACGCGCAAGGA
AGCGCAGGCGTATCTCGACTATCTGTGGTCGCCGGCCGCGCAGGAGATCATCGCGCAGCATCACTTGCGTCCGCAGGACA
AGACCGTGCTCGCGAAGCATGCGTCGGAGTTCAAGGCGATCAAAACCTTCACTGTCGAAGAGATGTTCGGTGGCTGGCAG
AAAGCGCAGCAAACGCATTTCTCCGATGGCGGGACATTCGATCAGATCATCGTCGACAAGAAGTAA

Upstream 100 bases:

>100_bases
GCGCACGCGGCACAGACCGAGTAAGCGATCCGTTAGAATCCCGCTTCACGCTGCACGTTCGTGCGGCCACTGGTTTTCAT
CAACGAAAGGCGGGGACAGG

Downstream 100 bases:

>100_bases
TTTCCGGCTTCACAAGTAAAAAGGCTGCCTCGTGGAAAACGAGGCAGCCTTTTTTATCGCGCGTGTTCTACTGCACGCGA
CGCATGCGCTCAGTTCGGCG

Product: sulfate ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAGKTGTSRWLAAGMTAMTLALAGVPAAHAETSLLNVSYDVTRELYKDINAGFIAAYKQKSGETVSIRQSHGASSAQALS
VLQGLQADVVTMNQPNDIDLLAEKGQLLPANWRTRLPNDSVPYTTTMVFLVRKGNPKHIKDWDDLARPGVQVIIANPKTS
GNGRYTYLAAWGYRKQHGGTDAQALDFEKAIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGVGGGNFEAV
YPSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSPAAQEIIAQHHLRPQDKTVLAKHASEFKAIKTFTVEEMFGGWQ
KAQQTHFSDGGTFDQIIVDKK

Sequences:

>Translated_341_residues
MAGKTGTSRWLAAGMTAMTLALAGVPAAHAETSLLNVSYDVTRELYKDINAGFIAAYKQKSGETVSIRQSHGASSAQALS
VLQGLQADVVTMNQPNDIDLLAEKGQLLPANWRTRLPNDSVPYTTTMVFLVRKGNPKHIKDWDDLARPGVQVIIANPKTS
GNGRYTYLAAWGYRKQHGGTDAQALDFEKAIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGVGGGNFEAV
YPSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSPAAQEIIAQHHLRPQDKTVLAKHASEFKAIKTFTVEEMFGGWQ
KAQQTHFSDGGTFDQIIVDKK
>Mature_340_residues
AGKTGTSRWLAAGMTAMTLALAGVPAAHAETSLLNVSYDVTRELYKDINAGFIAAYKQKSGETVSIRQSHGASSAQALSV
LQGLQADVVTMNQPNDIDLLAEKGQLLPANWRTRLPNDSVPYTTTMVFLVRKGNPKHIKDWDDLARPGVQVIIANPKTSG
NGRYTYLAAWGYRKQHGGTDAQALDFEKAIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGVGGGNFEAVY
PSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSPAAQEIIAQHHLRPQDKTVLAKHASEFKAIKTFTVEEMFGGWQK
AQQTHFSDGGTFDQIIVDKK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=320, Percent_Identity=54.375, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=50.3246753246753, Blast_Score=313, Evalue=8e-87,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37038; Mature: 36907

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKTGTSRWLAAGMTAMTLALAGVPAAHAETSLLNVSYDVTRELYKDINAGFIAAYKQK
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCEEEECHHHHHHHHHHHCCCCEEEEEECC
SGETVSIRQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLAEKGQLLPANWRTRLPNDS
CCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCC
VPYTTTMVFLVRKGNPKHIKDWDDLARPGVQVIIANPKTSGNGRYTYLAAWGYRKQHGGT
CCCEEEEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCC
DAQALDFEKAIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGVGGGNFEAV
CCHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCCCCEEE
YPSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSPAAQEIIAQHHLRPQDKTVLAKH
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHH
ASEFKAIKTFTVEEMFGGWQKAQQTHFSDGGTFDQIIVDKK
HHHHHHHHHEEHHHHHCCHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
AGKTGTSRWLAAGMTAMTLALAGVPAAHAETSLLNVSYDVTRELYKDINAGFIAAYKQK
CCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCEEEECHHHHHHHHHHHCCCCEEEEEECC
SGETVSIRQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLAEKGQLLPANWRTRLPNDS
CCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCCCC
VPYTTTMVFLVRKGNPKHIKDWDDLARPGVQVIIANPKTSGNGRYTYLAAWGYRKQHGGT
CCCEEEEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCC
DAQALDFEKAIFKNVPVLDTGGRGATTTFTQRGIGDVLVTFENEVALMDTGVGGGNFEAV
CCHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCCCCEEE
YPSVSLLAAPPVSIVDKVVDKRGTRKEAQAYLDYLWSPAAQEIIAQHHLRPQDKTVLAKH
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHH
ASEFKAIKTFTVEEMFGGWQKAQQTHFSDGGTFDQIIVDKK
HHHHHHHHHEEHHHHHCCHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]