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The map label for this gene is purR [H]

Identifier: 91781561

GI number: 91781561

Start: 207746

End: 208732

Strand: Reverse

Name: purR [H]

Synonym: Bxe_A4284

Alternate gene names: 91781561

Gene position: 208732-207746 (Counterclockwise)

Preceding gene: 91781562

Following gene: 91781560

Centisome position: 4.26

GC content: 68.79

Gene sequence:

>987_bases
ATGGCCACAACTATCCGCGACGTCGCCCGCGCGGCCAGCGTGTCGATCGGCACCGTCTCACGCGCATTGAAGAACCAGCC
GGGTCTCTCCGAGGCCACCCGCGAGCGCGTCGTCGAGGCGGCGCGCAAGCTCGGCTATGACGCCGCGCAATTGCGCCCGC
GCATCCGCCGTCTCACCTTTCTGCTGCACCGTCAGCACAACAATTTCGCTGTGAGCCCGTTCTTTTCGCACGTGCTGCAC
GGCGTGGAAGATGCCTGCCGCGAGCGCGGCATCGTGCCGTCCGTGCTGACCGCCGGCCCGACTGAAGACGTGATCCAGCA
AATGCGCCTGCATGCGCCGGATGCAGTGGCGATTGCCGGTTTCGTCGAACCGGAAACGCTCGCCACGCTGGTGGCCATGC
AGCGCCCGCTCGTGCTGATCGACCTGTGGGCGCCCGGCCTGCGCTCGGTGAATCTCGACAACGCCGCGGGCGCCACGCTT
GCCATGCGGCATCTGTTCGAACAGCAGCGCAAGCGCGTCGCGTTCATCGGCGGCTCGCTTGCGCACTTCAGCATCGCGCA
GCGGGCGCTCGGCTACCGGCGCGCGTTTTTCGAAGCGGGGCTGCTGTTCGACCCGTCGCTGGAAGTGACCATCGACGCAG
GCCTCGACCCCGCCAGCGGCGCCGCGCGCGCCATGCATCAACTGCTCGACGCACCGGGCCCGCGCCCCGACGCCGTGTTT
GCCTACAACGACGCCGCCGCGCTCGCCGCGCTGCGCGCATGCCTCGCGCGCGGCCTGCGCGTGCCCGAGGACATCGCCAT
CATCGGTTTCGACGACATTCCCGCCGCCGCGCATGCCACGCCGCCGCTGTCCACCATCTCGGTCGACAAGGAAGCGCTCG
GCCGGCGCGGCGTCGAGCTCCTGCTCGAAGACGCACCCGCCGAACCGGAAGTCCGTCTGCCCGTCCATCTCATTGCCCGT
GCCAGTACTTTGGTGAAAACGCCATGA

Upstream 100 bases:

>100_bases
TATCGATCGACGGATTCTCGGGCTGTTTTAGCACGGTTTTACTATACAATCGCCCGAACCCAAACACCGTGCGGCCGCCC
ACGCGCGCCCGCCTGATTCG

Downstream 100 bases:

>100_bases
CGCAATCCGATCCGCTACACCCCGCCAACGGCGCCGCCGCGGTGCCGCCCGTCGAGAGCTTCCGCTCGCGCGACTTTCTG
CTCTCGCACGTGCAGGACAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVVEAARKLGYDAAQLRPRIRRLTFLLHRQHNNFAVSPFFSHVLH
GVEDACRERGIVPSVLTAGPTEDVIQQMRLHAPDAVAIAGFVEPETLATLVAMQRPLVLIDLWAPGLRSVNLDNAAGATL
AMRHLFEQQRKRVAFIGGSLAHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPASGAARAMHQLLDAPGPRPDAVF
AYNDAAALAALRACLARGLRVPEDIAIIGFDDIPAAAHATPPLSTISVDKEALGRRGVELLLEDAPAEPEVRLPVHLIAR
ASTLVKTP

Sequences:

>Translated_328_residues
MATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVVEAARKLGYDAAQLRPRIRRLTFLLHRQHNNFAVSPFFSHVLH
GVEDACRERGIVPSVLTAGPTEDVIQQMRLHAPDAVAIAGFVEPETLATLVAMQRPLVLIDLWAPGLRSVNLDNAAGATL
AMRHLFEQQRKRVAFIGGSLAHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPASGAARAMHQLLDAPGPRPDAVF
AYNDAAALAALRACLARGLRVPEDIAIIGFDDIPAAAHATPPLSTISVDKEALGRRGVELLLEDAPAEPEVRLPVHLIAR
ASTLVKTP
>Mature_327_residues
ATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVVEAARKLGYDAAQLRPRIRRLTFLLHRQHNNFAVSPFFSHVLHG
VEDACRERGIVPSVLTAGPTEDVIQQMRLHAPDAVAIAGFVEPETLATLVAMQRPLVLIDLWAPGLRSVNLDNAAGATLA
MRHLFEQQRKRVAFIGGSLAHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPASGAARAMHQLLDAPGPRPDAVFA
YNDAAALAALRACLARGLRVPEDIAIIGFDDIPAAAHATPPLSTISVDKEALGRRGVELLLEDAPAEPEVRLPVHLIARA
STLVKTP

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=34.5679012345679, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=34.5047923322684, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=31.1764705882353, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789456, Length=316, Percent_Identity=30.6962025316456, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=31.6151202749141, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=29.8816568047337, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=27.0627062706271, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=26.2536873156342, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786540, Length=345, Percent_Identity=28.4057971014493, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787906, Length=309, Percent_Identity=28.4789644012945, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=23.4920634920635, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35268; Mature: 35137

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVVEAARKLGYDAAQLRPRIRRLTF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFAVSPFFSHVLHGVEDACRERGIVPSVLTAGPTEDVIQQMRLHAPDAVAIAG
HHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCEEEEC
FVEPETLATLVAMQRPLVLIDLWAPGLRSVNLDNAAGATLAMRHLFEQQRKRVAFIGGSL
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
AHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPASGAARAMHQLLDAPGPRPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AYNDAAALAALRACLARGLRVPEDIAIIGFDDIPAAAHATPPLSTISVDKEALGRRGVEL
EECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHCHHH
LLEDAPAEPEVRLPVHLIARASTLVKTP
HHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATTIRDVARAASVSIGTVSRALKNQPGLSEATRERVVEAARKLGYDAAQLRPRIRRLTF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LLHRQHNNFAVSPFFSHVLHGVEDACRERGIVPSVLTAGPTEDVIQQMRLHAPDAVAIAG
HHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCEEEEC
FVEPETLATLVAMQRPLVLIDLWAPGLRSVNLDNAAGATLAMRHLFEQQRKRVAFIGGSL
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
AHFSIAQRALGYRRAFFEAGLLFDPSLEVTIDAGLDPASGAARAMHQLLDAPGPRPDAVF
HHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AYNDAAALAALRACLARGLRVPEDIAIIGFDDIPAAAHATPPLSTISVDKEALGRRGVEL
EECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHCHHH
LLEDAPAEPEVRLPVHLIARASTLVKTP
HHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA