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The map label for this gene is citA [H]

Identifier: 91781514

GI number: 91781514

Start: 155684

End: 156976

Strand: Direct

Name: citA [H]

Synonym: Bxe_A4332

Alternate gene names: 91781514

Gene position: 155684-156976 (Clockwise)

Preceding gene: 91781513

Following gene: 91781517

Centisome position: 3.18

GC content: 63.34

Gene sequence:

>1293_bases
ATGACACCAGCGTCAAGGAGCCTTCATACGCGGGCGGTCGTCGCGGCTGTGATCGGCAATGCGCTCGAGTGGTACGACTT
CACCGTGTTCGGTTTTCTGACGGTCGTGATCGCGCAACTGTTCTTCCCGGCCGGCAACGACTATGCGTCGCTGCTCCTCA
CCACCGCAACGTTCGGCGTCGCTTTCGTCATGCGGCCGATCGGCGGCATCGTATTCGGACTCTACGCGGATCGCGCGGGA
CGCAAAGCGGCGTTGTCGCTCGTCATCGCGCTGATGACGCTCGGCATTCTGGTGCTCGCGATCGCACCGCCGTATTCGGC
GATCGGCATCGGCGCGCCGTTGATGATCGTAGTCGGACGCCTGCTGCAGGGCTTTTCCGCCGGCGGCGAGTTCGGCAGTT
CGACGGCGTTGCTGATCGAAGCGGCGCCATTCTCGAAGCGCGGCTTCTACGGCAGCTGGCAGATGGCGAGCCAGGCGGCG
GCGCTGCTGCTCGGCTCGCTCATGGGCGCATTGATCACGCGTGGGTTGTCGCCCGAAGCGTTGAAATCCTGGGGCTGGCG
CGTGCCGTTCATCCTGGGTTTGATCATCGGGCCGATCGGCTTTTATATCCGCCGGAATCTCGCTGACTCGGAAGCGTTCC
TGCTCGCGCAAAAAACCGCGCGGCGCGCGACGCTCGGCGAAGTCTTCAGAAGCCACAGCCGCGACGTGCTCTGCGGACTC
GGCTCGGTGATCGCACTCACGGTGACGATCTACGTGCTGATCAGCTATCTGCCGACCTTCGCGGTCCATCAACTGAAACT
GCCCTACGCACAATCCTTTTACGCGGTGATCGTCGGCAATCTGCTGTTGACGGTGCTTTCGCCGTTGACCGGCGCCTGGT
CGGACCGGATCGGGCGCAAGGGGCTGTCGCTGTGGTCGCTGGTGATCACGCTCGTGATCATCTATCCGCTGTTCGTGTGG
CTCGCGGCGGAGCCGAGCGTGTCGAAACTGATTCTCGTGCAGGCGTTGCTGTCGATCACGCTGTCCGGCTATTACGGGCC
GTTCGGCGCGCTGATTGCGGAGCTGTTTCCGGCGAATGTGCGCTCGATTGGACTATCGCTCGCGTACAACATCGCGGTGA
TGGTGTTCGGCGGGTTTGGGCCTTTTATCGTGACGTGGCTGATCGATACCACCGGCTCGCCGCTCGCGCCGACTTACTAC
GTGATGGGTGGACTGGCGCTGTCGATCGTCGCGGTGGCCTGCATTCCGGGCAAGCGCCACGCCGATCTCGACGCGCAGCG
CAAACCGGCCTGA

Upstream 100 bases:

>100_bases
TGCGACCTCAGCGGCCGGCGTCATTTTTCTCACTCGCGCCGATGCGCCATTGCACCGAAACGACGCACCACAGGATACTT
TTCGCAGGCTGGAGATTCGA

Downstream 100 bases:

>100_bases
GCGGCGGTTCGCATCCGGTTGCGCGTCGGTGTTCACTGCGGGACTTCGCCGCCACTTCGCATCGCTGCCCTTGGGTGCCT
TTGGTGCCTTGGATGCTTTC

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTPASRSLHTRAVVAAVIGNALEWYDFTVFGFLTVVIAQLFFPAGNDYASLLLTTATFGVAFVMRPIGGIVFGLYADRAG
RKAALSLVIALMTLGILVLAIAPPYSAIGIGAPLMIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAA
ALLLGSLMGALITRGLSPEALKSWGWRVPFILGLIIGPIGFYIRRNLADSEAFLLAQKTARRATLGEVFRSHSRDVLCGL
GSVIALTVTIYVLISYLPTFAVHQLKLPYAQSFYAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVIIYPLFVW
LAAEPSVSKLILVQALLSITLSGYYGPFGALIAELFPANVRSIGLSLAYNIAVMVFGGFGPFIVTWLIDTTGSPLAPTYY
VMGGLALSIVAVACIPGKRHADLDAQRKPA

Sequences:

>Translated_430_residues
MTPASRSLHTRAVVAAVIGNALEWYDFTVFGFLTVVIAQLFFPAGNDYASLLLTTATFGVAFVMRPIGGIVFGLYADRAG
RKAALSLVIALMTLGILVLAIAPPYSAIGIGAPLMIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAA
ALLLGSLMGALITRGLSPEALKSWGWRVPFILGLIIGPIGFYIRRNLADSEAFLLAQKTARRATLGEVFRSHSRDVLCGL
GSVIALTVTIYVLISYLPTFAVHQLKLPYAQSFYAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVIIYPLFVW
LAAEPSVSKLILVQALLSITLSGYYGPFGALIAELFPANVRSIGLSLAYNIAVMVFGGFGPFIVTWLIDTTGSPLAPTYY
VMGGLALSIVAVACIPGKRHADLDAQRKPA
>Mature_429_residues
TPASRSLHTRAVVAAVIGNALEWYDFTVFGFLTVVIAQLFFPAGNDYASLLLTTATFGVAFVMRPIGGIVFGLYADRAGR
KAALSLVIALMTLGILVLAIAPPYSAIGIGAPLMIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAA
LLLGSLMGALITRGLSPEALKSWGWRVPFILGLIIGPIGFYIRRNLADSEAFLLAQKTARRATLGEVFRSHSRDVLCGLG
SVIALTVTIYVLISYLPTFAVHQLKLPYAQSFYAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVIIYPLFVWL
AAEPSVSKLILVQALLSITLSGYYGPFGALIAELFPANVRSIGLSLAYNIAVMVFGGFGPFIVTWLIDTTGSPLAPTYYV
MGGLALSIVAVACIPGKRHADLDAQRKPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=32.512315270936, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788942, Length=376, Percent_Identity=34.3085106382979, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=34.4988344988345, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=29.2740046838407, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87082404, Length=373, Percent_Identity=26.2734584450402, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082231, Length=238, Percent_Identity=26.890756302521, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45739; Mature: 45608

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASRSLHTRAVVAAVIGNALEWYDFTVFGFLTVVIAQLFFPAGNDYASLLLTTATFGV
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AFVMRPIGGIVFGLYADRAGRKAALSLVIALMTLGILVLAIAPPYSAIGIGAPLMIVVGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
LLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLLGSLMGALITRGLSPEA
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LKSWGWRVPFILGLIIGPIGFYIRRNLADSEAFLLAQKTARRATLGEVFRSHSRDVLCGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSVIALTVTIYVLISYLPTFAVHQLKLPYAQSFYAVIVGNLLLTVLSPLTGAWSDRIGRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLSLWSLVITLVIIYPLFVWLAAEPSVSKLILVQALLSITLSGYYGPFGALIAELFPANV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
RSIGLSLAYNIAVMVFGGFGPFIVTWLIDTTGSPLAPTYYVMGGLALSIVAVACIPGKRH
HHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ADLDAQRKPA
CCCCCCCCCC
>Mature Secondary Structure 
TPASRSLHTRAVVAAVIGNALEWYDFTVFGFLTVVIAQLFFPAGNDYASLLLTTATFGV
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AFVMRPIGGIVFGLYADRAGRKAALSLVIALMTLGILVLAIAPPYSAIGIGAPLMIVVGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
LLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLLGSLMGALITRGLSPEA
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LKSWGWRVPFILGLIIGPIGFYIRRNLADSEAFLLAQKTARRATLGEVFRSHSRDVLCGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSVIALTVTIYVLISYLPTFAVHQLKLPYAQSFYAVIVGNLLLTVLSPLTGAWSDRIGRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLSLWSLVITLVIIYPLFVWLAAEPSVSKLILVQALLSITLSGYYGPFGALIAELFPANV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
RSIGLSLAYNIAVMVFGGFGPFIVTWLIDTTGSPLAPTYYVMGGLALSIVAVACIPGKRH
HHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ADLDAQRKPA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]