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The map label for this gene is metK [H]

Identifier: 91781475

GI number: 91781475

Start: 108858

End: 110048

Strand: Direct

Name: metK [H]

Synonym: Bxe_A4371

Alternate gene names: 91781475

Gene position: 108858-110048 (Clockwise)

Preceding gene: 91781468

Following gene: 91781477

Centisome position: 2.22

GC content: 63.64

Gene sequence:

>1191_bases
GTGGCAAACGACTATCTCTTCACTTCCGAATCCGTCTCCGAAGGCCATCCCGACAAAGTCGCGGATCAGATTTCCGACGC
CATTCTCGACGCCATCCTGGCTCAGGACAAATACTCGCGTGTCGCCGCGGAAACGCTGTGCAACACGGGTCTCGTCGTGC
TGGCCGGTGAAATCACCACGACCGCTAACGTCGATTACATCCAGGTCGCACGCAACACGATCAAGCGCATCGGTTACGAC
AACACCGACTACGGCATCGACTATCGCGGCTGCGCCGTGCTCGTCGCGTATGACAAGCAATCGCCGGACATCGCCCAGGG
CGTGGACCGCGCGCACGACAACAACCTCGATCAAGGCGCCGGCGACCAGGGCCTGATGTTCGGTTACGCGTGCGAAGAGA
CACCGGAACTGATGCCGTTGCCGATCCACCTGTCGCACCGTCTGGTGGAGCGTCAGGCGAATCTGCGCCGTGACGGCCGT
CTGCCCTGGCTGCGTCCGGACGCGAAGTCGCAGGTCACCGTGCGTTATGTCGACGGCAAACCGCATTCCATCGACACCGT
GGTGCTGTCCACGCAGCATTCGCCGGACATCGATCTGGGCACGCTGCGCGAAGCCGTGATCGAAGAAGTGATCAAGCCGA
CGCTGCCGGCCGACCTCATCAAGGGCGACATCAAGTTCCTCGTGAACCCGACCGGCCGTTTCGTGATTGGCGGTCCGCAA
GGCGATTGCGGCTTGACCGGCCGCAAGATCATCGTCGATACGTACGGCGGTGCCGCACCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGACCCGTCGAAGGTCGACCGTTCGGCGGCCTACGCCGGCCGTTATGTCGCGAAGAACATCGTGGCCGCGGGCC
TGGCTTCGCGCTGCCTGATCCAGGTTTCGTACGCGATCGGCGTCGCCCAGCCGACTTCGGTGATGGTGAACACGTTCGGC
ACCGGCCGCGTGTCGGATGCGGTCATCACGCGTCTCGTGCAGGAGCATTTCGACCTGCGTCCGAAGGGCATCATCCAGAT
GCTCGACCTGCTGCGCCCGATCTACGAGAAGAGCGCCGCCTATGGTCACTTCGGCCGCGAAGAGCCGGAATTCACGTGGG
AATCCACGGACAAGGCGCTGGCACTCGCCGAAGCCGCCGGCACGGAACCGGTTGCCGCGTTGGCCGAGTAA

Upstream 100 bases:

>100_bases
CGCCTGTCGGTAAGGTAATCCGCTAAAGCGTCGCCGCTTCAAGGCTCCCGAAGCTGGCTACGTGAAACTCAACCGTAACC
ACAAAACAGGAGTCTGCAAC

Downstream 100 bases:

>100_bases
GCGCAAGCCGTCCTTTCAAGTAAAAACCCCGCCCGGCGCGTTGAGCCGGGCGGGGTTTTTTTTATCGCCGTCGCCAGTCC
ATGAACTGAATGGCGACGTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHSPDIDLGTLREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFG
TGRVSDAVITRLVQEHFDLRPKGIIQMLDLLRPIYEKSAAYGHFGREEPEFTWESTDKALALAEAAGTEPVAALAE

Sequences:

>Translated_396_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHSPDIDLGTLREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFG
TGRVSDAVITRLVQEHFDLRPKGIIQMLDLLRPIYEKSAAYGHFGREEPEFTWESTDKALALAEAAGTEPVAALAE
>Mature_395_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARNTIKRIGYDN
TDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGRL
PWLRPDAKSQVTVRYVDGKPHSIDTVVLSTQHSPDIDLGTLREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLIQVSYAIGVAQPTSVMVNTFGT
GRVSDAVITRLVQEHFDLRPKGIIQMLDLLRPIYEKSAAYGHFGREEPEFTWESTDKALALAEAAGTEPVAALAE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=58.7765957446808, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=57.1052631578947, Blast_Score=415, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=69.9738903394256, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=59.3085106382979, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.9473684210526, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.7765957446808, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=55.3615960099751, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=52.9255319148936, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.9268292682927, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=57.2289156626506, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.1428571428571, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=60.7142857142857, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=56.4643799472295, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=57.1428571428571, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=54.7872340425532, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=54.2780748663102, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=54.2780748663102, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=55.4621848739496, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42724; Mature: 42592

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARNTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHSPDIDLGTLREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVSYAIGVAQPTSVMVNTFGTGRVSDAVITRLVQEHFDLRPKGIIQMLDLLRPIYEKSAA
EEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
YGHFGREEPEFTWESTDKALALAEAAGTEPVAALAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARNTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACEETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTVRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHSPDIDLGTLREAVIEEVIKPTLPADLIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRCLI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVSYAIGVAQPTSVMVNTFGTGRVSDAVITRLVQEHFDLRPKGIIQMLDLLRPIYEKSAA
EEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
YGHFGREEPEFTWESTDKALALAEAAGTEPVAALAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA