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The map label for this gene is topB [H]

Identifier: 91781427

GI number: 91781427

Start: 57407

End: 60073

Strand: Reverse

Name: topB [H]

Synonym: Bxe_A4419

Alternate gene names: 91781427

Gene position: 60073-57407 (Counterclockwise)

Preceding gene: 91781428

Following gene: 91781425

Centisome position: 1.23

GC content: 62.73

Gene sequence:

>2667_bases
ATGTCCAAAGCACTGATCATCGCCGAAAAGCCTTCCGTCGCGAACGACATCGCGCGCGCTTTGGGCGGCTTTACCAAGCA
TGACGAATACTACGAAAGCGACGAGTACGTCCTTTCCTCGGCAGTCGGCCACCTGCTGGAAATTGCCGCGCCCGAAGACT
ACGAAGTCAAGCGCGGCAAGTGGAGTTTCGCCAATCTGCCCGTCATCCCGCCGCACTTCGATCTCAACCCGATCGCCAAG
AGCGAGTCGCGCCTGAAGGTGCTGACCAAGCTGCTCAAGCGCAAGGACGTCGACCGTCTGATCAACGCATGCGACGCGGG
GCGCGAGGGCGAGCTGATATTCCGCCTGATCGCGCAGCACGCGAAAGCGAGGCAGCCGGTGCAGCGCCTGTGGCTGCAAT
CCATGACGGCGGGCGCGATCCGCGACGGCTTCGCCCGTCTGCGCAGCGACGAAGAGATGCAACCGCTCGCCGATGCCGCA
CGCTGCCGCTCGGAGGCGGACTGGCTGGTCGGCATCAACGGCACGCGGGCCATGACCGCGTTCAACAGCAAGGGCGGCGG
CTTCTTCCTGACCACCGTCGGGCGGGTGCAAACGCCAACGCTGTCGATCGTGGTCGAGCGCGAAGAGAAGATTCGCCGCT
TCGTGCCGCGCGACTATTGGGAAGTCAAGGCGGAGTTCGTCTGCTCGGCCGGCTTCTACGAGGGCCGCTGGTTCGATCCG
AAATTCAAGCGCGACGAGTTCGATCCGGAAAAACGTGATTCGCGCCTGTGGTCGCTACCCGCGGCCGAGACGATCGTCGC
CGCGTGCCGTGGCCAGATCGGCACGGTGACTGAGGAATCGAAGCCGTCCACGCAACTGTCGCCGGCGCTCTTCGACCTGA
CCAGCTTGCAGCGGGAGGCCAACGGCCGCTTCGGCTTCTCGGCGAAGAACACCCTGGGCCTCGCCCAGGCGCTGTACGAA
AAGCACAAGGTGCTGACCTATCCCCGTACCGACGCGCGCGCGCTGCCGGAAGACTATATGGATACGGTCAAAGAGACGCT
CGGCATGCTCAAGGAGAGCAACAACTATCTGCCGTTCGCGAAGCAGGTGCTCGACAAGGGCTGGGTGAAGCCGAACAAGC
GCATCTTCGACAACTCGAAGATCAGCGACCACTTCGCCATCATCCCGACACTGCAAGCGCCGAAGAACCTGTCCGAGCCG
GAACAGAAGCTCTACGACCTCGTCGTGAAGCGATTCCTGTCGGTGTTCTTCCCCGCCGCCGAATTCAAGGTGACGACGCG
GATCACGGAAGTGGTCGGCCATCACTTCAAGACCGAAGGCAAGGTGCTGGTCGAGCCGGGCTGGTTGCAGGTCTACGGCC
GCGAGATCAGCGGCGAAGACGCGAACCTCGTGCCGGTGCAGAAGGACGAGAAGGTCAAGACGGACAGGATCGCCGCCCAG
CAACTGGTGACGAAACCGCCCGCACGCTACAACGAAGCCACCTTGCTGTCGGCTATGGAAGGCGCAGGCAAACTCGTCGA
AGACGACGAGTTGCGTGAAGCGATGGCCGCCAAAGGGCTCGGCACGCCGGCCACGCGCGCGGCGATCATCGAAGGTTTGC
TGGGTGAAAAGTATCTGATTCGCGAAGGCCGCGATCTGATTCCGACCGCCAAGGCTTTCCAGCTCATGACGCTGTTGCGC
GGTCTCGGCGTGAAGGAACTGACGGCGCCGGAATTGACCGGCGAGTGGGAATACAAGCTCTCGCAAATGGAACGCGGCAA
CCTGCCGCGCGACGCGTTCATGCAGGAAATCGCCCGCATGACGCAGACCATCGTGAAGCGCGCGAAAGAGTACGACTCCG
ACACGATCCCGGGCGACTACGCAACGCTGGAAACGCCGTGTCCGAATTGCGGCGGTCAGGTGAAGGAGAACTACCGGCGC
TTTGCCTGCTCGAAGTGCGAGTTCTCGATTTCGAAGATTCCGGGCGGACGCCAGTTCGAGATTCCGGAAGTCGAAGAGTT
GCTGCAGAACAAGACGATCGGGCCGCTGTCGGGTTTCCGCAGCAAGATGGGCCGGCCGTTCTCGGCGATCCTGAAGCTCT
CGTTCGACGACGAGATCAAGAACTACAAGCTCGAATTCGACTTCGGCCAGGATGCCGGCGGCGAAGACGGCGAACCGCCC
GACTTCTCCGATCAGCAGCCGGTCGGCGCGTGCCCGAAGTGCAAGGGCCGGGTGTTCGAGCACGGCATGAGCTACGTCTG
CGAGAACTCGGTCGCCAATCCGAAGACTTGCGACTTCCGCTCCGGCAAGGTGATCCTGCAGCAGGAAATCGCCCGCGAGC
AGATGGCCAAGCTGCTCGAAGAAGGCCGCACGGACCTGCTGACGAACTTCAAGTCGTCGCGAACCGGCCGGAATTTCAAG
GCATTCCTCGTCAAGCAGAACGACGGCAAGATCGGCTTCGAGTTCGAGAAGAAGGAGCCGTCGGCGAAGACTGTCGCGAA
GACCGCGGCGGCCAAGGCAGCGGCGGAAGCGGCGGATTCGGAGTCCGAGGACGGCGGAGCGCCGGTGGCCGTGAAGGCAG
CGCCGGCAGCAAAGAAGGCTGCGGCGAAAAAAGCGCCCGCAACCAAAACGGCGGCAGCCAGGAAAGCGCCCGCCAGGAAA
ACAGCGGCGCGTAAAACCGGCTCGTAA

Upstream 100 bases:

>100_bases
AGCGGTCGTTAATGGGTCGCAAAGGTCGCAAACGAGGGTCGCGCTGATTAAAAGCGCACTCACGGCCATCAACCCTGATC
TACCAACCTCACATCGAGTC

Downstream 100 bases:

>100_bases
TCGAACCGGCGGCGGAAAGGAGATTCGCTTTCCCGCCGCCTGGTTTGAGGCTTGCCACGAAAAAAGGCGCGGTCACACAA
ATGACCGCGCCTTTTTTCGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGKWSFANLPVIPPHFDLNPIAK
SESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKARQPVQRLWLQSMTAGAIRDGFARLRSDEEMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREANGRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEP
EQKLYDLVVKRFLSVFFPAAEFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDRIAAQ
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLIREGRDLIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARMTQTIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACSKCEFSISKIPGGRQFEIPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSFDDEIKNYKLEFDFGQDAGGEDGEPP
DFSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLEEGRTDLLTNFKSSRTGRNFK
AFLVKQNDGKIGFEFEKKEPSAKTVAKTAAAKAAAEAADSESEDGGAPVAVKAAPAAKKAAAKKAPATKTAAARKAPARK
TAARKTGS

Sequences:

>Translated_888_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGKWSFANLPVIPPHFDLNPIAK
SESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKARQPVQRLWLQSMTAGAIRDGFARLRSDEEMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREANGRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEP
EQKLYDLVVKRFLSVFFPAAEFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDRIAAQ
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLIREGRDLIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARMTQTIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACSKCEFSISKIPGGRQFEIPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSFDDEIKNYKLEFDFGQDAGGEDGEPP
DFSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLEEGRTDLLTNFKSSRTGRNFK
AFLVKQNDGKIGFEFEKKEPSAKTVAKTAAAKAAAEAADSESEDGGAPVAVKAAPAAKKAAAKKAPATKTAAARKAPARK
TAARKTGS
>Mature_887_residues
SKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGKWSFANLPVIPPHFDLNPIAKS
ESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKARQPVQRLWLQSMTAGAIRDGFARLRSDEEMQPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDPK
FKRDEFDPEKRDSRLWSLPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREANGRFGFSAKNTLGLAQALYEK
HKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPE
QKLYDLVVKRFLSVFFPAAEFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDRIAAQQ
LVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLIREGRDLIPTAKAFQLMTLLRG
LGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARMTQTIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRF
ACSKCEFSISKIPGGRQFEIPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSFDDEIKNYKLEFDFGQDAGGEDGEPPD
FSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLEEGRTDLLTNFKSSRTGRNFKA
FLVKQNDGKIGFEFEKKEPSAKTVAKTAAAKAAAEAADSESEDGGAPVAVKAAPAAKKAAAKKAPATKTAAARKAPARKT
AARKTGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=653, Percent_Identity=25.8805513016845, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI4507635, Length=704, Percent_Identity=22.5852272727273, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=34.248788368336, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1787529, Length=670, Percent_Identity=25.9701492537313, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=623, Percent_Identity=26.0032102728732, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=24.1324921135647, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585251, Length=644, Percent_Identity=25.9316770186335, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640096, Length=716, Percent_Identity=21.927374301676, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98557; Mature: 98426

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGK
CCCCEEEECCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
WSFANLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AKARQPVQRLWLQSMTAGAIRDGFARLRSDEEMQPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVKRFLSVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
EFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDRIAAQ
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHH
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLI
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRDLIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARM
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQTIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACSKCEFSISKIPGGRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCEEHHHCCCCCCCC
IPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSFDDEIKNYKLEFDFGQDAGGEDGEPP
CHHHHHHHHCCCCCCHHHHHHHHCCCHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCC
DFSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EGRTDLLTNFKSSRTGRNFKAFLVKQNDGKIGFEFEKKEPSAKTVAKTAAAKAAAEAADS
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCC
ESEDGGAPVAVKAAPAAKKAAAKKAPATKTAAARKAPARKTAARKTGS
CCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure 
SKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPEDYEVKRGK
CCCEEEECCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
WSFANLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AKARQPVQRLWLQSMTAGAIRDGFARLRSDEEMQPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCSAGFYEGRWFDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRGQIGTVTEESKPSTQLSPALFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYMDTVKETLGMLKESNNYLPFA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVKRFLSVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
EFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDRIAAQ
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHH
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLI
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRDLIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARM
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQTIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACSKCEFSISKIPGGRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCEEHHHCCCCCCCC
IPEVEELLQNKTIGPLSGFRSKMGRPFSAILKLSFDDEIKNYKLEFDFGQDAGGEDGEPP
CHHHHHHHHCCCCCCHHHHHHHHCCCHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCC
DFSDQQPVGACPKCKGRVFEHGMSYVCENSVANPKTCDFRSGKVILQQEIAREQMAKLLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EGRTDLLTNFKSSRTGRNFKAFLVKQNDGKIGFEFEKKEPSAKTVAKTAAAKAAAEAADS
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCC
ESEDGGAPVAVKAAPAAKKAAAKKAPATKTAAARKAPARKTAARKTGS
CCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA