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The map label for this gene is actVB [H]

Identifier: 91781394

GI number: 91781394

Start: 14424

End: 15005

Strand: Reverse

Name: actVB [H]

Synonym: Bxe_A4453

Alternate gene names: 91781394

Gene position: 15005-14424 (Counterclockwise)

Preceding gene: 91781395

Following gene: 91781390

Centisome position: 0.31

GC content: 61.17

Gene sequence:

>582_bases
ATGCTGGCCACTCAGTTCCGCGATATCTTCAGCGCACTCGCCGCAGGTGTCTCGGTCATTACTTTCGACGCCGGCGGCCA
CGTCCACGGATTCACGGCGACGTCGCTGACGCCCGTGTCACTGATGCCGCCGCTCGCATTGTTTTGCGTCGCGCGAAGCA
GCAGGAGCGGCGCCTCGCTGTCGCACGGCAAGATGATCGGCATATCGATCCTTGGCGAGACGCAAAGGCATGTGTCGCGG
CGTTTTTCCGAACAGGCGAAGCCCGGCAGCTATCGGGACATTACGGTAAGGCGCAACGAGGACGGCGTTCCGGTGGTCAG
CGGCGCCAACGCGACGATAGAAGCGCGGATCGCCAATATATATCCGGCCGGCGATCACCTGATCTGCGTTTGTGAATTGA
AGTCGGGTTGTCTGACGCAGGCGGACGAGCCGCTTCTGTATTTCTCGCGGCGCTACCATGGGGTTTCGGTGTTGCCCGAG
TTGGAAACTGAGCGGGAACTTGCGCCGCAGAAGGAAGAAGTGGAAGAGGCAGCGGTTTGCGGTGATTCTGACGGACGTGT
GCAGGGTAGGCGAGCCTGCTGA

Upstream 100 bases:

>100_bases
TGAACTGTCTTCGGCCGGTCGGACCGATCTGGTCGATTGGCTGCGGGCAGTTCGCCGGCAGACGCCGGGCATCGTCGGGT
TTGCTCAAGAGGAGAGCTTC

Downstream 100 bases:

>100_bases
TTGCCGGCTTGGCTTGATGGGAAGGCGTAATGAGGCCACGCGCATGGACCGTCTCAGGCCTGAATAATCGTATCCTGTTG
ATTTGCGCCGCGAACTGACC

Product: putative flavin-dependent reductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: actI ORF6 [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MLATQFRDIFSALAAGVSVITFDAGGHVHGFTATSLTPVSLMPPLALFCVARSSRSGASLSHGKMIGISILGETQRHVSR
RFSEQAKPGSYRDITVRRNEDGVPVVSGANATIEARIANIYPAGDHLICVCELKSGCLTQADEPLLYFSRRYHGVSVLPE
LETERELAPQKEEVEEAAVCGDSDGRVQGRRAC

Sequences:

>Translated_193_residues
MLATQFRDIFSALAAGVSVITFDAGGHVHGFTATSLTPVSLMPPLALFCVARSSRSGASLSHGKMIGISILGETQRHVSR
RFSEQAKPGSYRDITVRRNEDGVPVVSGANATIEARIANIYPAGDHLICVCELKSGCLTQADEPLLYFSRRYHGVSVLPE
LETERELAPQKEEVEEAAVCGDSDGRVQGRRAC
>Mature_193_residues
MLATQFRDIFSALAAGVSVITFDAGGHVHGFTATSLTPVSLMPPLALFCVARSSRSGASLSHGKMIGISILGETQRHVSR
RFSEQAKPGSYRDITVRRNEDGVPVVSGANATIEARIANIYPAGDHLICVCELKSGCLTQADEPLLYFSRRYHGVSVLPE
LETERELAPQKEEVEEAAVCGDSDGRVQGRRAC

Specific function: Catalyzes The Reduction Of Free Flavins (Fmn, FAD And Riboflavin) By NADH (By Similarity). [C]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.pristinaespiralis snaC [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=152, Percent_Identity=26.3157894736842, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20760; Mature: 20760

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATQFRDIFSALAAGVSVITFDAGGHVHGFTATSLTPVSLMPPLALFCVARSSRSGASL
CCCHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
SHGKMIGISILGETQRHVSRRFSEQAKPGSYRDITVRRNEDGVPVVSGANATIEARIANI
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCEEEEEEEEE
YPAGDHLICVCELKSGCLTQADEPLLYFSRRYHGVSVLPELETERELAPQKEEVEEAAVC
ECCCCCEEEEEECCCCCCCCCCCCCEEEHHHCCCEEECCCCCCHHHCCCCHHHHHHHHHC
GDSDGRVQGRRAC
CCCCCCCCCCCCC
>Mature Secondary Structure
MLATQFRDIFSALAAGVSVITFDAGGHVHGFTATSLTPVSLMPPLALFCVARSSRSGASL
CCCHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
SHGKMIGISILGETQRHVSRRFSEQAKPGSYRDITVRRNEDGVPVVSGANATIEARIANI
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCEEEEEEEEE
YPAGDHLICVCELKSGCLTQADEPLLYFSRRYHGVSVLPELETERELAPQKEEVEEAAVC
ECCCCCEEEEEECCCCCCCCCCCCCEEEHHHCCCEEECCCCCCHHHCCCCHHHHHHHHHC
GDSDGRVQGRRAC
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1527048; 12000953 [H]