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The map label for this gene is radA [H]

Identifier: 91214105

GI number: 91214105

Start: 5049558

End: 5050988

Strand: Direct

Name: radA [H]

Synonym: UTI89_C5160

Alternate gene names: 91214105

Gene position: 5049558-5050988 (Clockwise)

Preceding gene: 162138296

Following gene: 91214106

Centisome position: 99.68

GC content: 57.51

Gene sequence:

>1431_bases
TTGCCTGCCCGTCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTG
TAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGCCTGTCATGCCTGGAACACCATCACCGAGGTGC
GTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCTATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAG
AAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTTCGACCGCGTACTAGGCGGCGG
CGTGGTGCCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGTGCCGGGAAATCCACGCTGCTGTTGCAAACGCTGTGCA
AACTGGCGCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTT
GGCCTGCCGACTGACAATCTTAATATGCTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACC
GAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGTACAATCATCGCCTGGCAGCGTGGCACAGGTGC
GTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTCGGGCACGTAACCAAAGAT
GGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGTGATGCCGACTCACGTTT
TCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGTGTCTTCGCAATGACCGAGCAGGGGCTGC
GTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTAGTGTGG
GAAGGAACGCGTCCGCTGCTGGTGGAGATTCAGGCGCTCGTCGATCACTCGATGATGGCGAATCCACGCCGCGTGGCAGT
CGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTTCACCGTCACGGTGGTCTACAAATGGCCGATCAGGATG
TGTTTGTTAACGTGGTCGGTGGCGTGAAGGTGACGGAAACCAGTGCCGATTTAGCGTTACTGCTGGCGATGGTTTCCAGC
CTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAG
CGGCCAGGAACGAATCTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCAATTGTTCCGGCAGCTAACGTGCCGAAAAAAG
CGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAA

Upstream 100 bases:

>100_bases
TGCCAAGCCAAAAGTGAATGAAAAAACGGAAGTCACCATCCGTCACGCTGACCTGATGGGTGTATTCTGCATCCTCTCTG
GCAGCCTGAATCAGAAGTAA

Downstream 100 bases:

>100_bases
TGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACTCTACAGCAG
GTAGCTGATGCCAGCGGTAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPARHPAGGTALTRYTVAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQ
KLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRL
GLPTDNLNMLSETSIEQICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKD
GSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVW
EGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSS
LRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Sequences:

>Translated_476_residues
MPARHPAGGTALTRYTVAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQ
KLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRL
GLPTDNLNMLSETSIEQICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKD
GSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVW
EGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSS
LRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL
>Mature_475_residues
PARHPAGGTALTRYTVAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQK
LSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLG
LPTDNLNMLSETSIEQICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDG
SLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWE
GTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSL
RDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=99.5652173913043, Blast_Score=936, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 51093; Mature: 50961

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARHPAGGTALTRYTVAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
CCCCCCCCCCHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCHHHHHCHHHHEEEECCCC
VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNP
HHCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCC
GAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIEQICL
CCCHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHEEE
IAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKD
EECCCCCCEEEEECEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCC
GSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPS
CCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCEECHHHHHHHCCCC
AIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAV
EEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAG
HHHCCCCEEECCCEEEEEECCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
EIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL
CCCCCCCHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
PARHPAGGTALTRYTVAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
CCCCCCCCCHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCHHHHHCHHHHEEEECCCC
VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNP
HHCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCC
GAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIEQICL
CCCHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHEEE
IAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKD
EECCCCCCEEEEECEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCC
GSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPS
CCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCEECHHHHHHHCCCC
AIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAV
EEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
LHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAG
HHHCCCCEEECCCEEEEEECCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
EIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL
CCCCCCCHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]