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The map label for this gene is gldA [H]

Identifier: 91213973

GI number: 91213973

Start: 4924163

End: 4925245

Strand: Direct

Name: gldA [H]

Synonym: UTI89_C5025

Alternate gene names: 91213973

Gene position: 4924163-4925245 (Clockwise)

Preceding gene: 91213969

Following gene: 91213974

Centisome position: 97.21

GC content: 45.8

Gene sequence:

>1083_bases
ATGGATAAGATTATTATAAGTCCGGCTAAATATATCCAGGGAAATGGTTCTCTGGATAATATTGCTACATATGCGGCGAG
TTTGGGAACAGAACCATTAATTATTGCAGATGAATTTGTGACCGGTCTGGTTGGTGACAGAGTAAGCCAGAGCTTTGCAA
GAGAAAACATTATTGCGGATTTTGATGTTTTTTGCGGTGAATGCTCGCAAAATGAAATTTCTCGAATTCGTAAGAAATTT
AATCAGCGTAAATATAATGTCGTTATTGGTATTGGCGGCGGTAAAACGTTGGATACGGCAAAAGCCGTAGCGTATTACCA
AAAAATTCCGGTGGTGGTGGTGCCGACAATTGCTTCCACAGATGCGCCAACCAGTTCTCTGGCAGTAATTTACACCCCGG
ATGGTCAGTTTAGCGAATATCTGTTTTTCCCGAAAAACCCGGATATGGTGATTATGGATACTGGCGTCATTTCAGCCGCG
CCAGTGCGTCTGCTGGTGGCCGGTATGGGAGATGCATTGTCAACCTGGTTTGAGGCGAGAGCCAATCAGGCGTCAGGAAA
AGCCACAATGGCAGGTGGGGCGTCGACACTGGCGGCACTGGCTATTGCCCGACTCTGTTATACCACGCTACTGGAAGATG
GCTATAAGGCAAAAGTCGCGGTTGAACAAGGTGTTAGCACCAAAGCGGTAGAGAATATTATCGAAGCTAATACCTATCTT
AGCGGCATTGGATTTGAAAGTAGCGGCCTGGCGGCAGCTCATGCCATTCATAACGGCATGACGCAACTGGAGGAATGTCA
TCATTGCTACCACGGTGAAAAAGTGGCATTTGGCGTCCTGGTACAGCTCGTACTGGAAAACGCACCACAGGAGGAAATTG
AAACCGTACTCAATTTCTGCCATAGCGTTGGCTTGCCGACAAATCTTCATATGTTAGGCGTGAAGGAAATTAATAAAGAT
AAATTACGGGCAGTGGCTAAAGCCGCGACTGCTGAAGGGGAAACTATCCATAATATGCCATTCGTAGTAACAGCGCAGGA
CGTTCTTTGTGCAATTTTAACTGCACATCATTTGGGACTTTAA

Upstream 100 bases:

>100_bases
GCGATCATAAAACATAATTTTTCGCGAATGAAATGTTTGCCGCCACTGGATGCAACGAGTAGGTTAACAGTGAAGCAAAA
CAAGTTACGCAAGGAATGAT

Downstream 100 bases:

>100_bases
AAAATAATACGGCCTGTGCAAAGCAGGCCGTTGGTGTCATTTTAAATACTTCATTCGGGAATAAATATGACCAGTAACTG
GAAACGTTGGATCACGCTGG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLVGDRVSQSFARENIIADFDVFCGECSQNEISRIRKKF
NQRKYNVVIGIGGGKTLDTAKAVAYYQKIPVVVVPTIASTDAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAA
PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAKVAVEQGVSTKAVENIIEANTYL
SGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVAFGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKD
KLRAVAKAATAEGETIHNMPFVVTAQDVLCAILTAHHLGL

Sequences:

>Translated_360_residues
MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLVGDRVSQSFARENIIADFDVFCGECSQNEISRIRKKF
NQRKYNVVIGIGGGKTLDTAKAVAYYQKIPVVVVPTIASTDAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAA
PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAKVAVEQGVSTKAVENIIEANTYL
SGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVAFGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKD
KLRAVAKAATAEGETIHNMPFVVTAQDVLCAILTAHHLGL
>Mature_360_residues
MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLVGDRVSQSFARENIIADFDVFCGECSQNEISRIRKKF
NQRKYNVVIGIGGGKTLDTAKAVAYYQKIPVVVVPTIASTDAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAA
PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAKVAVEQGVSTKAVENIIEANTYL
SGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVAFGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKD
KLRAVAKAATAEGETIHNMPFVVTAQDVLCAILTAHHLGL

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=359, Percent_Identity=56.8245125348189, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786815, Length=361, Percent_Identity=29.6398891966759, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38408; Mature: 38408

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLVGDRVSQSFARENIIAD
CCCEEECHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCC
FDVFCGECSQNEISRIRKKFNQRKYNVVIGIGGGKTLDTAKAVAYYQKIPVVVVPTIAST
HHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCC
DAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAAPVRLLVAGMGDALSTWFEAR
CCCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
ANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAKVAVEQGVSTKAVENIIEANTYL
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHH
SGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVAFGVLVQLVLENAPQEEIETVLNFC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
HSVGLPTNLHMLGVKEINKDKLRAVAKAATAEGETIHNMPFVVTAQDVLCAILTAHHLGL
HHCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLVGDRVSQSFARENIIAD
CCCEEECHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCC
FDVFCGECSQNEISRIRKKFNQRKYNVVIGIGGGKTLDTAKAVAYYQKIPVVVVPTIAST
HHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCC
DAPTSSLAVIYTPDGQFSEYLFFPKNPDMVIMDTGVISAAPVRLLVAGMGDALSTWFEAR
CCCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
ANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAKVAVEQGVSTKAVENIIEANTYL
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHHH
SGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVAFGVLVQLVLENAPQEEIETVLNFC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
HSVGLPTNLHMLGVKEINKDKLRAVAKAATAEGETIHNMPFVVTAQDVLCAILTAHHLGL
HHCCCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA