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The map label for this gene is nrfE [H]

Identifier: 91213617

GI number: 91213617

Start: 4560882

End: 4562564

Strand: Direct

Name: nrfE [H]

Synonym: UTI89_C4664

Alternate gene names: 91213617

Gene position: 4560882-4562564 (Clockwise)

Preceding gene: 91213616

Following gene: 91213618

Centisome position: 90.03

GC content: 56.8

Gene sequence:

>1683_bases
TTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGAT
GCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCA
TCAGCGATTTCTCGGTCATTTATGTCGCCCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAACTGGCGGCGGTGTGG
GGCGGTCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGTGCTGTTTGCCTGGCATTATCGACA
GCAAACCGATCCGCTATTTCCGCTCACGTTAACGGTTTTATCGCTGATACTTGCCGCACTGCTGCTGTTTGTTGTCGGAT
GGTCCGATCCTTTTGTGAGGATATTTCCGCCCGCAATAGAAGGGCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTT
ATCTTTCATCCACCGCTGCTTTATCTCGGCTACGGCGGTTTGATGGTGGCGGCGAGCGTGGCGCTGGCGAGTTTACTGCG
CGGCGAGTTTGGTGGTACCTGCGCCCGAATTTGCTGGCGTTGGGCACTACCTGGCTGGAGCGCATTAACAGCGGGGATCA
TCCTCGGTTCCTGGTGGGCCTATTGCGAACTGGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCGTCGTTA
TTACCCTGGCTTTCTGCCACAGCGCTGCTGCACAGTTTATCCCTGACACGCCAGCGGGGGATTTTCCGCCACTGGTCGCT
GTTGCTGGCGATAGTAACTCTGATGCTGTCGCTGCTGGGCACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATG
CGTTCGCGCTGGATAACGTCCGCGCCGTGCCGTTGTTCAGCCTGTTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTAT
GGCTGGCGAGCGCGGGACGGTGGCCCGGCGGTGCGTTTTTCGGGGTTATCGCGGGAAATGTTAATCCTCGCTACGCTGTT
GCTGTTTTGCGCAGTGCTACTGATCGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGCCTGCTGGGCTGGGGACGCC
TCTCCGTTGGCGCGCCGTATTTTAACCGCGCGACGTTACCGTTTGCTCTGTTGATGCTGGTGGTGATTGTGCTGGCGACG
TTTGTCTCTGGCAAACGTGCTCAGCTTCCGGCGCTGCTGGCGCATGCGGGCGTGCTGTTATTTGCCGCGGGGATCGTGGT
TTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTT
TTGAGCGCCTCGATCTGCAAGCCAAAGGCAATTACACCAGTGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGC
ATTGGTGAATTAACGCCGGAGCGGCGTTTTTACGAAGCACGTCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAATGG
CATCCATGACTGGTATGCGGTCATGGGCGAGAAAACCGGAGCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTG
TGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGAAGGAAGCGCGATGAA
TAA

Upstream 100 bases:

>100_bases
CTGCGCTTTTTCATTCTCTACGCCGGGCAGTTGACGGTGGCGTAAGCCAGAACAGAGGTGGTTTCTGGACGTATTCCTTC
CTGAAGTCGGTTTTCTGGCG

Downstream 100 bases:

>100_bases
AGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTTGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGCAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVW
GGHEGSLLLWVLLLSAWSVLFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGL
IFHPPLLYLGYGGLMVAASVALASLLRGEFGGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASL
LPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALY
GWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLAT
FVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQR
IGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Sequences:

>Translated_560_residues
MLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVW
GGHEGSLLLWVLLLSAWSVLFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGL
IFHPPLLYLGYGGLMVAASVALASLLRGEFGGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASL
LPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALY
GWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLAT
FVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQR
IGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE
>Mature_560_residues
MLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVW
GGHEGSLLLWVLLLSAWSVLFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGL
IFHPPLLYLGYGGLMVAASVALASLLRGEFGGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASL
LPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALY
GWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLAT
FVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQR
IGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=552, Percent_Identity=97.1014492753623, Blast_Score=992, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=584, Percent_Identity=38.3561643835616, Blast_Score=337, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 62189; Mature: 62189

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVI
CEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YVAQHSYSLLSWELKLAAVWGGHEGSLLLWVLLLSAWSVLFAWHYRQQTDPLFPLTLTVL
EEEECCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHH
ALASLLRGEFGGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASL
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCC
LPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCC
RAVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLL
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQR
HHHHHHHHCCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHH
IGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGG
HHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECC
GLLMIAGALLSGWRGRKRDE
CHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVI
CEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YVAQHSYSLLSWELKLAAVWGGHEGSLLLWVLLLSAWSVLFAWHYRQQTDPLFPLTLTVL
EEEECCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHH
ALASLLRGEFGGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASL
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCC
LPWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCC
RAVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLL
HHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQR
HHHHHHHHCCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHH
IGELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGG
HHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECC
GLLMIAGALLSGWRGRKRDE
CHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]