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The map label for this gene is rbsR [H]

Identifier: 91213280

GI number: 91213280

Start: 4199095

End: 4200087

Strand: Direct

Name: rbsR [H]

Synonym: UTI89_C4308

Alternate gene names: 91213280

Gene position: 4199095-4200087 (Clockwise)

Preceding gene: 162138329

Following gene: 91213287

Centisome position: 82.89

GC content: 50.15

Gene sequence:

>993_bases
TTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTT
CGTCAGTGAAGCGATTACAGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCC
TCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGC
GTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTTGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGTAA
TCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAGCCTTCGCGTGAAATCA
TGCAACGTTATCCGACAGTGCCTACCGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAAC
TCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCATACCCGTATTGCCTGTATTACCGGTCCGCT
GGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCT
ATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCCATGCGCCAACTGTTATCACATCCGCTGCGTCCTCAG
GCTGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGA
TATCGCGGTGATTGGCTATGACGATATCGAACTGGCAAGCTTTATGACGCCACCATTAACAACTATCCACCAACCGAAAG
ATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGGCCCTACAGCAACAACGATTACAACTT
ACTCCGATTCTGATGGAACGTGGTTCGGCTTAG

Upstream 100 bases:

>100_bases
CCATGCAGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTT
TAGACAGGCAGAGGTGACGC

Downstream 100 bases:

>100_bases
ATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGTAGCATGAACATTGCTGCTGAAGCAACAGTAATA
ATGCCCATCGTGATAAACGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPALQQQRLQL
TPILMERGSA

Sequences:

>Translated_330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPALQQQRLQL
TPILMERGSA
>Mature_329_residues
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQA
VFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPALQQQRLQLT
PILMERGSA

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=99.6969696969697, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=46.969696969697, Blast_Score=321, Evalue=3e-89,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.5987055016181, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.4470989761092, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=30.3514376996805, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.5483870967742, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=29.5081967213115, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=27.6872964169381, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=28.6585365853659, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=25.8461538461538, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=27.5862068965517, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=26.2987012987013, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=26.813880126183, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790192, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=24.1590214067278, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36582; Mature: 36451

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPALQQQRLQLTPILMERGSA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPALQQQRLQLTPILMERGSA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]