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The map label for this gene is yhiE [H]

Identifier: 91213017

GI number: 91213017

Start: 3925067

End: 3925672

Strand: Direct

Name: yhiE [H]

Synonym: UTI89_C4043

Alternate gene names: 91213017

Gene position: 3925067-3925672 (Clockwise)

Preceding gene: 91213016

Following gene: 91213018

Centisome position: 77.48

GC content: 34.49

Gene sequence:

>606_bases
TTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATGTTCGAAACGATAACGGCTAAGGAGCAAGTTAT
GATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAATCA
ATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTGATCATTGACACCTATCACAACCATATCCTTGAT
GAAGAAGCGATTAAATTCCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTAAA
AGCTAAAGCGCCTATTTATTTTGTCAGCCGTAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGC
CCCATAAGAATTCACAACTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAACAAAAATGAAAGC
AATATCACGTCAACGCTCAACATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAATTAAG
ACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAG

Upstream 100 bases:

>100_bases
CCAATATATGGAGTGTGTGATGGATAAATCTGAAGTATTGATTAGTGTTAATAGACGTATTAGTTCACGAAGGGCAAAGT
TCTTATAGGCGTTTACTATA

Downstream 100 bases:

>100_bases
TAAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAATACCCCCGGTGATTATTG
AAGGAGAGACGAAAACGACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNNDSDKDVNNEKDDMFETITAKEQVMIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILD
EEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNES
NITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF

Sequences:

>Translated_201_residues
MNNDSDKDVNNEKDDMFETITAKEQVMIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILD
EEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNES
NITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF
>Mature_201_residues
MNNDSDKDVNNEKDDMFETITAKEQVMIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILD
EEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNES
NITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF

Specific function: Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci. Co

COG id: COG2771

COG function: function code K; DNA-binding HTH domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789928, Length=175, Percent_Identity=100, Blast_Score=354, Evalue=2e-99,
Organism=Escherichia coli, GI1789923, Length=167, Percent_Identity=23.9520958083832, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991
- InterPro:   IPR013022 [H]

Pfam domain/function: PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 23613; Mature: 23613

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDSDKDVNNEKDDMFETITAKEQVMIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIK
CCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEEECCHHHHH
KVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKES
HHCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHH
IKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNI
HHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECHHHHEEEEEEEE
MYKLKLRRMSDIVTLGITSYF
EEEEEHHHHHHHHEEEEECCC
>Mature Secondary Structure
MNNDSDKDVNNEKDDMFETITAKEQVMIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIK
CCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEEECCHHHHH
KVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKES
HHCCCCEEEEEEHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHH
IKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNI
HHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECHHHHEEEEEEEE
MYKLKLRRMSDIVTLGITSYF
EEEEEHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]