Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 91212799

GI number: 91212799

Start: 3710211

End: 3712118

Strand: Direct

Name: thiC [H]

Synonym: UTI89_C3825

Alternate gene names: 91212799

Gene position: 3710211-3712118 (Clockwise)

Preceding gene: 91212798

Following gene: 91212800

Centisome position: 73.24

GC content: 55.08

Gene sequence:

>1908_bases
TTGGAACGAGCTATGTCTGCAACAAAACTGACCCGCCGCGAACAACGCGCCCAGGCCCAACATTTTATCGACACCCTGGA
AGGCTCCGCCTTTCCCAACTCAAAACGCATTTACATCACTGGCACACACCCCGGCGTACGCGTGCCGATGCGTGAGATTC
AGCTTAGCCCGACGCTAATTGGCGGCAGCAAAGAACAGCCGCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACC
TCCGGCCCGTATGGCGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGC
GCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACAAAAGCGCGGCTGGCAGATGATGGCCTCGACGAGC
TGCGTTTTAGCGGCGTATTAACCCCAAAACGCGCCAAAGCAGGCCACCGCGTCACACAACTGCACTACGCCCGCAAGGGT
ATCATCACGCCAGAAATGGAATTCATCGCCATTCGCGAGAATATGGGTCGCGAGCGCATACGTAGCGAAGTTTTACGCCA
CCAGCATCCGGGAATGAGCTTTGGCGCACGTCTGCCGGAAAATATCACTGCGGAATTTGTCCGTGATGAAGTTGCTGCCG
GACGTGCGATTATCCCGGCCAACATTAATCATCCGGAATCGGAGCCGATGATTATTGGCCGTAACTTCCTAGTAAAAGTT
AACGCCAATATCGGCAACTCAGCGGTCACTTCTTCCATCGAAGAAGAAGTGGAAAAACTGGTATGGTCCACACGCTGGGG
AGCGGATACCGTGATGGATCTTTCCACTGGTCGCTATATTCACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGC
CGATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGC
GACACCCTGCTGGAACAAGCCGAGCAAGGTGTGGATTACTTCACGATCCATGCGGGGGTGCTGCTGCGCTACGTGCCGAT
GACCGCGAAACGCCTGACCGGTATCGTCTCTCGCGGCGGTTCGATTATGGCGAAATGGTGCCTCTCCCATCATCAGGAAA
ATTTCCTCTATCAACGCTTCCGCGAAATTTGTGAAATCTGTGCTGCTTATGACGTTTCGCTATCGCTGGGCGACGGCCTG
CGCCCCGGTTCTATTCAGGACGCCAACGATGAAGCACAGTTTGCCGAGCTGCATACGCTGGGTGAACTGACTAAAATCGC
CTGGGAATATGACGTACAGGTGATGATTGAAGGCCCAGGCCACGTGCCGATGCAGATGATTCGCCGCAATATGACCGAGG
AGTTAGAGCACTGCCACGAAGCGCCGTTTTACACTCTGGGGCCGCTAACTACCGATATCGCGCCGGGCTATGACCACTTC
ACGTCAGGGATTGGCGCGGCGATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGG
CCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGATTGCCGCTCACGCCGCCGACCTGGCGAAAGGGC
ATCCGGGCGCGCAAATTCGCGATAACGCCATGTCGAAAGCCCGCTTCGAATTTCGCTGGGAAGACCAGTTTAATCTGGCC
CTCGACCCGTTTACTGCCCGCGCTTATCACGATGAAACCCTGCCGCAAGAGTCCGGTAAAGTCGCCCATTTTTGCTCCAT
GTGTGGACCGAAATTCTGCTCGATGAAAATCAGCCAGGAAGTGCGTGATTACGCCGCCGCGCAAACCATTGAAGTGGGAA
TGGCGGATATGTCGGAGAACTTCCGCGCCAGAGGCGGAGAAATCTACCTGCGTAAGGAGGAAGCGTGA

Upstream 100 bases:

>100_bases
ATACCTGCGAAGGGAACAAGAGTTAATCTGCTATCGCATCGCCTTTGCGGCGATCGTCTCTTGCTTCATCCGTCGTCTGA
CAAGCCACGTCCTTAACTTT

Downstream 100 bases:

>100_bases
TGTATCAGCCTGAGTTTCCTCCTGTACCCTTTCGTTTAGGACTGTACCCGGTGGTTGACAGCGTACAGTGGATCGAACGT
CTGTTGGATGCAGGCGTACG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MERAMSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDT
SGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKG
IITPEMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKV
NANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFR
DTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQRFREICEICAAYDVSLSLGDGL
RPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHF
TSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLA
LDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA

Sequences:

>Translated_635_residues
MERAMSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDT
SGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKG
IITPEMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKV
NANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFR
DTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQRFREICEICAAYDVSLSLGDGL
RPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHF
TSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLA
LDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA
>Mature_635_residues
MERAMSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLIGGSKEQPQYEENEAIPVYDT
SGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKG
IITPEMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKV
NANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFR
DTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQRFREICEICAAYDVSLSLGDGL
RPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHF
TSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLA
LDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=631, Percent_Identity=98.5736925515055, Blast_Score=1301, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 71282; Mature: 71282

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERAMSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEE
GGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSD
CCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCC
YTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITPEMEFIAIRENMGRERI
CCCHHHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHH
RSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEE
NANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHH
YQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
SIMAKWCLSHHQENFLYQRFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
GELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHF
HHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHH
TSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIR
HHHHHHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DNAMSKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQE
HHHHHHCEEEEEECCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
VRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MERAMSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRVPMREIQLSPTLI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEE
GGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSD
CCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCC
YTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITPEMEFIAIRENMGRERI
CCCHHHCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHH
RSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEE
NANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHH
YQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
SIMAKWCLSHHQENFLYQRFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
GELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHF
HHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHH
TSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIR
HHHHHHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DNAMSKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQE
HHHHHHCEEEEEECCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
VRDYAAAQTIEVGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA