| Definition | Escherichia coli UTI89 chromosome, complete genome. |
|---|---|
| Accession | NC_007946 |
| Length | 5,065,741 |
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The map label for this gene is purH [H]
Identifier: 91212788
GI number: 91212788
Start: 3698818
End: 3700407
Strand: Direct
Name: purH [H]
Synonym: UTI89_C3814
Alternate gene names: 91212788
Gene position: 3698818-3700407 (Clockwise)
Preceding gene: 91212780
Following gene: 91212789
Centisome position: 73.02
GC content: 55.72
Gene sequence:
>1590_bases ATGCAACAACGTCGTCCAGTCCGCCGCGCTCTGCTCAGTGTTTCTGACAAAGCCGGTATCGTCGAATTCGCCCAGGCACT TTCCGCACGCGGTGTGGAGCTGCTGTCTACAGGGGGCACAGCCCGTCTGTTAGCAGAAAAAGGTCTGCCGGTAACCGAAG TTTCCGATTACACCGGTTTCCCGGAGATGATGGATGGACGCGTGAAGACCCTGCATCCGAAAGTACATGGTGGAATTCTG GGCCGTCGCGGCCAGGACGATACCATTATGGAAGAACATCAGATCCAGCCTATCGATATGGTGGTTGTTAACCTGTATCC GTTCGCCCAGACCGTTGCTCGTGAAGGCTGCTCGCTGGAAGATGCGGTTGAGAACATCGATATCGGCGGCCCGACGATGG TGCGCTCTGCCGCCAAGAACCATAAAGATGTCGCAATCGTGGTGAAGAGCAGCGACTACGACGCCATTATTAAAGAGATA GATGCCAATGAAGGCTCCCTGACTCTGGAAACTCGCTTTGACCTTGCCATCAAAGCCTTCGAACACACCGCCGCCTACGA CAGCATGATTGCCAACTACTTCGGCAGCATGGTTCCGGCTTACCACGGTGAAAGCAAAGAAGCCGCCGGTCGCTTCCCAC GCACGCTGAACCTGAACTTCATTAAGAAGCAGGATATGCGTTACGGCGAGAACAGCCACCAGCAGGCTGCCTTCTATATA GAAGAGAATGTGAAAGAAGCCTCCGTTGCTACTGCAACCCAGGTTCAAGGTAAAGCCCTCTCTTATAACAACATCGCCGA CACCGATGCGGCGCTGGAGTGCGTGAAAGAGTTCGCCGAGCCGGCATGTGTGATTGTGAAACACGCCAACCCTTGCGGCG TGGCTATCGGCAATTCCATTCTTGATGCTTACGATCGCGCGTACAAAACCGACCCAACCTCCGCATTCGGCGGCATCATT GCCTTTAACCGCGAACTGGATGCTGAAACCGCGCAGGCCATCATTTCTCGTCAGTTCGTCGAAGTGATTATTGCGCCGTC CGCCAGCGAAGAAGCCCTGAAAATCACCGCCGCCAAGCAAAACGTACGCGTTCTGACCTGCGGTCAGTGGGGCGAGCGTG TTCCGGGTCTTGATTTCAAACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGCGATCTGGGGATGGTCGGTGCAGAAGAA CTGCGCGTCGTCACCAAACGCCAGCCGACCGAACAGGAATTGCGTGATGCGCTGTTCTGCTGGAAAGTGGCGAAGTTCGT GAAATCCAACGCTATCGTCTATGCCAAAAACAATATGACCATCGGTATTGGCGCGGGCCAGATGAGCCGCGTGTACTCCG CGAAAATCGCCGGTATTAAAGCCGCCGATGAAGGTCTGGAAGTGAAAGGTTCCTCGATGGCTTCTGACGCGTTCTTCCCG TTCCGCGACGGTATTGATGCCGCCGCCGCTGCGGGCGTGACCTGTGTAATCCAGCCTGGCGGTTCCATCCGTGATGACGA AGTGATTGCCGCCGCCGACGAGCACGGTATTGCGATGCTCTTCACCGACATGCGCCACTTCCGCCATTAA
Upstream 100 bases:
>100_bases CGGGCGAAAAATAAGGATGCCCCGTTAGGGGCGTTAGCTGAGTTTTTCGCGAAAAATTCAGCTAACGCTCTCTGTAATAG TCAAATCCAGGGGATTTACC
Downstream 100 bases:
>100_bases TGGAGCAATAGATGAAAGTATTAGTGATTGGTAACGGCGGGCGCGAGCACGCGCTGGCCTGGAAAGCGGCTCAGTCGCCG CTGGTTGAAACTGTTTTTGT
Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
Products: NA
Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]
Number of amino acids: Translated: 529; Mature: 529
Protein sequence:
>529_residues MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE LRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
Sequences:
>Translated_529_residues MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE LRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH >Mature_529_residues MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE LRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]
COG id: COG0138
COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the purH family [H]
Homologues:
Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=39.3814432989691, Blast_Score=280, Evalue=2e-75, Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=98.8657844990548, Blast_Score=1079, Evalue=0.0, Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=33.8762214983713, Blast_Score=294, Evalue=9e-80, Organism=Caenorhabditis elegans, GI71985574, Length=354, Percent_Identity=27.4011299435028, Blast_Score=94, Evalue=1e-19, Organism=Caenorhabditis elegans, GI71985556, Length=141, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6323056, Length=490, Percent_Identity=35.3061224489796, Blast_Score=270, Evalue=5e-73, Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=35.8762886597938, Blast_Score=267, Evalue=3e-72, Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=35.0245499181669, Blast_Score=306, Evalue=2e-83,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002695 - InterPro: IPR013982 - InterPro: IPR016193 - InterPro: IPR011607 [H]
Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]
EC number: =2.1.2.3; =3.5.4.10 [H]
Molecular weight: Translated: 57385; Mature: 57385
Theoretical pI: Translated: 5.53; Mature: 5.53
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC PEMMDGRVKTLHPKVHGGILGRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLE HHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEIDANEGSLTLETRFDLAIKAF HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHH EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSI ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTKRQPTEQELRDALFC CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC >Mature Secondary Structure MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC PEMMDGRVKTLHPKVHGGILGRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLE HHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEIDANEGSLTLETRFDLAIKAF HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHH EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSI ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTKRQPTEQELRDALFC CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA