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The map label for this gene is fhlA [H]

Identifier: 91212091

GI number: 91212091

Start: 3013395

End: 3015509

Strand: Direct

Name: fhlA [H]

Synonym: UTI89_C3094

Alternate gene names: 91212091

Gene position: 3013395-3015509 (Clockwise)

Preceding gene: 91212090

Following gene: 91212092

Centisome position: 59.49

GC content: 51.82

Gene sequence:

>2115_bases
TTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAGCAAGGGTTGTT
CGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTG
CGCTCGCCGACAACGCGGCGATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCATCGCGTGAAAAAGAC
ACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCA
TTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGCCACAGGTGGGCTATACCCCAAATTTGGTCACTATTGCC
TGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAA
GAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGT
CGACTATGAGTTGTTATGCCGGGAACGCGATAACTTCCGCATCCTGGTCGCCATCACTAACGCGGTGCTTTCCCGCCTGG
ATATGGACGAACTGGTCAGCGAAGTCGCCAAAGAAATCCATTACTATTTCGACATTGACGATATCAGCATCGTCTTACGC
AGCCACCGTAAAAACAAACTCAACATCTACTCCACTCACTATCTTGATAAACAGCATCCCGCTCACGAACAGAGCGAAGT
CGATGAAGCCGGAACCCTCACCGAACGCGTCTTCAAAAGTAAAGAGATGCTGTTGATTAATCTCCACGAGCGGGACGATT
TAGCCCCCTATGAACGCATGTTGTTCAACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGC
GACACTATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTCTTTACCACTACCAATCTGAATTTACTGCGCCA
GATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATTCATCGTCTGAAAGAACGGCTGGTTG
ATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATG
TACAGCGTACTTAAACAAGTGGAAATGGTGGCGCAAAGTGACAGTACCGTTCTGATCCTCGGTGAAACTGGTACAGGTAA
AGAGCTGATTGCCCGCGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGC
CTGCCGGATTGCTGGAAAGCGATCTATTTGGTCATGAGCGTGGGGCTTTTACCGGTGCCAGCGCTCAGCGCATTGGTCGT
TTTGAACTGGCGGATAAAAGCTCACTATTCCTCGATGAAGTGGGCGATATGCCGCTGGAGTTACAGCCGAAGTTGCTGCG
TGTATTACAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTACGTTTAATTGCCGCGACTA
ACCGCGATCTGAAAAAAATGGTCGCCGACCGAGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATCCAC
CTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGG
GCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCATCCTGAGCAACATGGAATGGCCGGGCAACGTGCGTGAGCTGG
AAAACGTCATCGAGCGCGCGGTATTGCTAACACGCGGCAACGTGCTGCAGCTGTCATTGCCAGATATTGCTTTACCAGAA
CCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCAGGAGGGCGAAGATGAATATCAGTTGATTGTTCGCGTGCTGAAAGA
AACAAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACTCTGCTGTCACGAATGA
AGCGACTGGGAATTGATAAATCGGCATTGATTTAA

Upstream 100 bases:

>100_bases
CTGTATGGCGTGAAACGAACCCTCGATCTACCGCACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCC
TATAGTTAGTCAATGACCTT

Downstream 100 bases:

>100_bases
CAGATGTGGTTATACTCGATCAACATTCAATGAAAGGAGGCAGTATGGCAGTTTCAGCAAGAAATCAATTAACCGGAACA
GTTAGCGCAGTAGCAATGGG

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MHRFAVLSWKNKMSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKD
TPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEK
EFNRLQTFTQIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLR
SHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFNTWGNQIQTLCLLPLMSG
DTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAM
YSVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGR
FELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIH
LPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPE
PETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Sequences:

>Translated_704_residues
MHRFAVLSWKNKMSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKD
TPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEK
EFNRLQTFTQIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLR
SHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFNTWGNQIQTLCLLPLMSG
DTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAM
YSVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGR
FELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIH
LPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPE
PETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
>Mature_704_residues
MHRFAVLSWKNKMSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKD
TPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEK
EFNRLQTFTQIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLR
SHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFNTWGNQIQTLCLLPLMSG
DTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAM
YSVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGR
FELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIH
LPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPE
PETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=692, Percent_Identity=99.1329479768786, Blast_Score=1413, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=566, Percent_Identity=51.0600706713781, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=46.2732919254658, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=46.9055374592834, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87082152, Length=366, Percent_Identity=39.344262295082, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1790299, Length=365, Percent_Identity=41.0958904109589, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=47.9508196721311, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081872, Length=265, Percent_Identity=44.9056603773585, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.855421686747, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=36.6366366366366, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.8267716535433, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.9018404907975, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 79952; Mature: 79952

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFAVLSWKNKMSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAA
CCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
IVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQL
EEEEECCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHH
ATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQ
HCCCCCCCCCCEEEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLR
HHHHCCCCCEEEEECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHH
SHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERM
HCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
LFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNAL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLIL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
GETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGR
ECCCCCHHHHHHHHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCHHHCCC
FELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKM
EEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHH
VADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRI
HHHHHHHHCCEEEEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQL
HHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHH
IVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
HHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MHRFAVLSWKNKMSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAA
CCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
IVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQL
EEEEECCHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHH
ATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQ
HCCCCCCCCCCEEEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLR
HHHHCCCCCEEEEECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHH
SHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERM
HCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
LFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNAL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLIL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
GETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGR
ECCCCCHHHHHHHHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCHHHCCC
FELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKM
EEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHH
VADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRI
HHHHHHHHCCEEEEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQL
HHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHH
IVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
HHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]