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The map label for this gene is emrB [H]

Identifier: 91212047

GI number: 91212047

Start: 2969345

End: 2970892

Strand: Direct

Name: emrB [H]

Synonym: UTI89_C3046

Alternate gene names: 91212047

Gene position: 2969345-2970892 (Clockwise)

Preceding gene: 91212046

Following gene: 91212048

Centisome position: 58.62

GC content: 56.33

Gene sequence:

>1548_bases
GTGCGTGTGATGCAACAGCAAAAACCGCTGGAAGGCGCACAACTGGTCATTATGACGATTGCGCTGTCACTGGCGACATT
CATGCAGGTGCTGGACTCCACCATTGCTAACGTGGCGATCCCCACTATCGCCGGGAATCTGGGCTCATCACTCAGTCAGG
GAACGTGGGTGATCACTTCTTTCGGGGTGGCGAATGCCATCTCGATCCCGCTTACCGGCTGGCTGGCAAAGCGCGTCGGG
GAAGTGAAATTGTTCCTCTGGTCAACCATCGCCTTTGCTATTGCGTCGTGGGCGTGTGGTGTCTCCAGCAGCCTGAATAT
GCTGATCTTCTTCCGCGTGATTCAGGGGATTGTCGCCGGGCCGCTGATCCCGCTTTCACAAAGTCTATTGCTGAATAACT
ACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTTGCACCAATTTGCGGCCCGATCCTC
GGCGGTTATATCAGTGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGAT
GACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTA
TTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTCTCATCACAGGAAATTATCATCCTC
ACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT
GTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCGATTGTTCTGC
TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTGGGCAGGGCTGGCCTCTGCGCCGGTAGGGATTATTCCG
GTGATCCTGTCGCCGATTATCGGCCGCTTTGCGCATAAACTGGATATGCGCCGACTGGTAACCTTCAGCTTTATTATGTA
TGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCGGGTATGGATTTTGGCGCGTCGGCCTGGCCACAGTTTATCC
AGGGTTTTGCGGTGGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTGTCCGGTTTGCCACCGGAACGACTGGCGGCG
GCATCGAGCCTCTCCAACTTTACGCGAACGCTGGCGGGGTCTATCGGCACGTCGATAACCACGACCATGTGGACCAACCG
CGAGTCACTGCACCATGCACAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCTCAGGCGATGTACAGTCAACTGG
AAGGGCTTGGTATGACGCAACAGCAGGCATCAGGCTGGATTGCCCAGCAGATCACCAATCAGGGGCTGATTATTTCCGCC
AATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCTTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCCTTTGGCGC
AGGTGGCGGCGGAGGCGGTGCGCACTAA

Upstream 100 bases:

>100_bases
TCCACACCGGTCGCGGTAAGCACCGCGCGTGAAATCAGCCTGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGC
TAACGCTGGCTAATCCAGAG

Downstream 100 bases:

>100_bases
GGTTGTCAAAGGGAGCTGCGGCTCCCTTTTTATTTGCTCATACTGTAAACGGTTGTTTAATCCACTCATTCGGTGTATCT
TTTCGCCGCCCCAAACTCTT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRVMQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVG
EVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPIL
GGYISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIIL
TVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Sequences:

>Translated_515_residues
MRVMQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVG
EVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPIL
GGYISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIIL
TVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
>Mature_515_residues
MRVMQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVG
EVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPIL
GGYISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIIL
TVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIP
VILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAA
ASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=512, Percent_Identity=99.8046875, Blast_Score=1027, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=62.4254473161034, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=31.924882629108, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.2079207920792, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=27.0833333333333, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6322958, Length=532, Percent_Identity=22.5563909774436, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=26.1980830670927, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55994; Mature: 55994

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEE
FGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAG
CCCHHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPI
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHH
GVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIIL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHH
TVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQE
HHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEP
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
GMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
TTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
EEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEEC
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVMQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEE
FGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAG
CCCHHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPI
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHH
GVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIIL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHH
TVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQE
HHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEP
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
GMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
TTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISA
EEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEEC
NEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]