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The map label for this gene is nupC [H]

Identifier: 91211736

GI number: 91211736

Start: 2674275

End: 2675516

Strand: Direct

Name: nupC [H]

Synonym: UTI89_C2725

Alternate gene names: 91211736

Gene position: 2674275-2675516 (Clockwise)

Preceding gene: 91211733

Following gene: 91211738

Centisome position: 52.79

GC content: 50.16

Gene sequence:

>1242_bases
GTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGT
TGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAAAATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAG
TGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTCTGGGCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTG
CTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGT
GCTGTGCCCAATCGTCTTTATCTCTGCGCTGATCGGTATTCTCCAGCATATTCGCGTATTGCCGGTGATTATCCGCGCAA
TTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAG
TCTGAAAACTTTATTGCCTATAAAGATATCCTCGGCAAAATCTCCCGCAATCGTATGTACACCATGGCAGCAACGGCGAT
GTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGACCATGCTGGAGCCGAAATACGTCGTTGCGGCGCTGGTACTGA
ACATGTTCAGCACCTTTATCGTGCTGTCGCTGATCAACCCTTACCGTGTTGATGCCAGTGAAGAAAATATTCAGATGTCC
AACCTGCACGAAGGTCAGAGCTTCTTCGAAATGCTGGGTGAATACATTCTGGCAGGTTTCAAGGTTGCCATTATCGTTGC
CGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTGACTGGCTGGTTTGGCTACA
GCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCATGGGTGATGGGTGTTCCTTCCAGTGAAGCACTG
CAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCAC
GCTCTCTCCGCGTGCGGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCGG
GTGCGGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAACTGGTTTACGGCTCTACCCTG
GTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAA

Upstream 100 bases:

>100_bases
TAGCAGAAATGTATGACAGATCACTATTTTTGAAGCTTGTCACAGGACGTCATTATATTGTGTGTCAGATCTCGTTTTCC
TTAACCATGTTACATAGAAT

Downstream 100 bases:

>100_bases
GACCATACATAAAAAAGCCGGGGATAATTCCCCGGCTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGACGACCAATT
AAATATCCTTGCAGATATTG

Product: nucleoside permease NupC

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MHGNLTCLIFGANMDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKL
LGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQ
SENFIAYKDILGKISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMS
NLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEAL
QVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTL
VSVLSASIAALVL

Sequences:

>Translated_413_residues
MHGNLTCLIFGANMDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKL
LGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQ
SENFIAYKDILGKISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMS
NLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEAL
QVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTL
VSVLSASIAALVL
>Mature_413_residues
MHGNLTCLIFGANMDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKL
LGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQ
SENFIAYKDILGKISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMS
NLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEAL
QVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTL
VSVLSASIAALVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=395, Percent_Identity=25.8227848101266, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI11545853, Length=406, Percent_Identity=24.1379310344828, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI227116277, Length=403, Percent_Identity=22.0843672456576, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=100, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=414, Percent_Identity=32.1256038647343, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788488, Length=413, Percent_Identity=30.5084745762712, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=393, Percent_Identity=24.4274809160305, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=392, Percent_Identity=25.2551020408163, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45552517, Length=391, Percent_Identity=25.8312020460358, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921868, Length=391, Percent_Identity=25.8312020460358, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45552519, Length=391, Percent_Identity=25.8312020460358, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI281360430, Length=344, Percent_Identity=24.7093023255814, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 44849; Mature: 44849

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGNLTCLIFGANMDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFF
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGI
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHH
TMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMS
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
NLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
LGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHGNLTCLIFGANMDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFF
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGI
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHH
TMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMS
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
NLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
LGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]