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The map label for this gene is ddg [H]

Identifier: 91211721

GI number: 91211721

Start: 2656846

End: 2657832

Strand: Direct

Name: ddg [H]

Synonym: UTI89_C2710

Alternate gene names: 91211721

Gene position: 2656846-2657832 (Clockwise)

Preceding gene: 91211719

Following gene: 91211723

Centisome position: 52.45

GC content: 50.96

Gene sequence:

>987_bases
TTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATTCGTATCTACAGAAGGTAACTATGTTTCCAAAATG
CAAATTTTCCCGCGAGTTTCTGCATCCTCGCTACTGGCTCACGTGGTTTGGGCTTGGTGTACTCTGGCTTTGGGTACAGC
TTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATCGGCACAATGGCGCGACCATTCCTGAAACGTCGTGAATCTATC
GCCCGTAAAAACCTGGAACTTTGTTTCCCGCAGCATTCTGCGAAAGAGCGCGAGAAGATGATTGCCGAAAACTTTCGTTC
ACTCGGCATGGCGCTGGTAGAAACCGGCATGGCGTGGTTCTGGCCCGACAGTCGCGTACGTAAATGGTTTGATGTGGAAG
GGCTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTG
GGCGGTCGGGTGATGGGACTTTGCCAACCAATGATGGCCACCTATCGCCCGCATAATAATCAGCTGATGGAATGGGTGCA
GACCCGTGGTCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGTATTGTCAGTGCACTGAAGAAAG
GTGAAGCGGTATGGTTTGCTCCCGATCAGGATTATGGTCGTAAAGGCAGCTCCTTCGCGCCGTTCTTTGCGGTGGAAAAT
GTCGCCACAACCAATGGCACCTATGTCCTCTCTCGTCTCTCTGGCGCAGCCATGTTGACCGTAACGATGGTAAGAAAAGC
GGATTACAGCGGATATCGTTTGTACATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCAAGCCGCTGCCTATA
TGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCG
GTGGGTGAATCGTCGTTATACATTTAA

Upstream 100 bases:

>100_bases
GATAAAGTACAGATACCGCATTCATGCTCCTCGTGGTTATGTCCTGACTAGTCTTTACACTCTTTACAGGAACCATTGTC
GTACATGATGGCCTAATCAA

Downstream 100 bases:

>100_bases
CAAACGGCTCTCACTCTTTCCCTCCTCAAATCAGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTT
CCTTCGCTGAATATCTAATT

Product: lipid A biosynthesis palmitoleoyl acyltransferase

Products: KDO2-(palmitoleoyl)-lipid IVA [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSVPLSTWNLLRYNNSYLQKVTMFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESI
ARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLEL
GGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRP
VGESSLYI

Sequences:

>Translated_328_residues
MSVPLSTWNLLRYNNSYLQKVTMFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESI
ARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLEL
GGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRP
VGESSLYI
>Mature_327_residues
SVPLSTWNLLRYNNSYLQKVTMFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGTMARPFLKRRESIA
RKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELG
GRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVENV
ATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPV
GESSLYI

Specific function: Unknown

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI87082094, Length=306, Percent_Identity=98.3660130718954, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=301, Percent_Identity=54.8172757475083, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=26.0586319218241, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: NA

Molecular weight: Translated: 38179; Mature: 38048

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPLSTWNLLRYNNSYLQKVTMFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLC
CCCCCCCHHHHHCCCHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
FLGTRIGTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWF
HHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
WPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNN
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCH
QLMEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
HHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECC
VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKIIEK
CCCCCCCEEEEHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EIMRAPEQYLWIHRRFKTRPVGESSLYI
HHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SVPLSTWNLLRYNNSYLQKVTMFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLC
CCCCCCHHHHHCCCHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
FLGTRIGTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWF
HHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
WPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNN
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCH
QLMEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
HHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECC
VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKIIEK
CCCCCCCEEEEHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EIMRAPEQYLWIHRRFKTRPVGESSLYI
HHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; palmitoleoyl-ACP [C]

Specific reaction: KDO2-lipid IVA + palmitoleoyl-ACP = KDO2-(palmitoleoyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]