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The map label for this gene is fliM [H]

Identifier: 91211163

GI number: 91211163

Start: 2046139

End: 2047188

Strand: Direct

Name: fliM [H]

Synonym: UTI89_C2145

Alternate gene names: 91211163

Gene position: 2046139-2047188 (Clockwise)

Preceding gene: 91211162

Following gene: 91211164

Centisome position: 40.39

GC content: 52.76

Gene sequence:

>1050_bases
ATGTCACCGACGTGCTGTATACCGCTTTTATTCTGCGATAACGACATGGGCGATAGTATTCTTTCTCAAGCTGAAATTGA
TGCGCTGTTGAATGGCGACAGCGAAGTCAAAGACGAACCGACAGCCAGTGTTAGCGGCGAAAGTGACATTCGTCCGTACG
ATCCGAATACCCAACGACGGGTTGTGCGCGAACGTTTGCAGGCGCTGGAAATCATTAATGAGCGCTTTGCTCGCCATTTT
CGTATGGGGCTGTTCAACCTGCTGCGCCGTAGCCCGGATATTACCGTCGGGGCCATCCGCATTCAGCCGTACCATGAATT
TGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATTCATCTGAAACCGCTGCGCGGCACAGGGCTGGTGGTGTTCT
CGCCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCGATGGACGCTTCCCGACCAAAGTGGAAGGCCGCGAG
TTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCATGGAAGGCGAT
TAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCAAACGACATTG
TGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAG
CCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCG
CCAGGTGCAGCATTCACAGCTGGAACTGGTGGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA
AACCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTTCTGACCAGTCAG
TACGGCACTCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACA
GCCCAAATGA

Upstream 100 bases:

>100_bases
GATGCTGCCGTACTGGCGACAGAAGAAGGCAAGAAAAACCTGATTGCCGAGATTAAAACCACACTTTCCACCCCGCTTGT
TGCCGGGCAACCGAAACAGG

Downstream 100 bases:

>100_bases
GTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAGCAAAAATCA
ACCAGCGGAAAAAGCGCTGC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSPTCCIPLLFCDNDMGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHF
RMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGRE
FTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIE
PLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQ
YGTLNGQYALRIEHLINPILNSLNEEQPK

Sequences:

>Translated_349_residues
MSPTCCIPLLFCDNDMGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHF
RMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGRE
FTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIE
PLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQ
YGTLNGQYALRIEHLINPILNSLNEEQPK
>Mature_348_residues
SPTCCIPLLFCDNDMGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHFR
MGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREF
THTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEP
LRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQY
GTLNGQYALRIEHLINPILNSLNEEQPK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=334, Percent_Identity=99.7005988023952, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 39518; Mature: 39387

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTCCIPLLFCDNDMGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRR
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEE
HLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGY
EEECCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
SDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIE
HHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHH
PLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLP
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
CCCCCCEEEEECCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPTCCIPLLFCDNDMGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRR
CCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEE
HLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGY
EEECCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
SDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIE
HHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHH
PLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLP
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
CCCCCCEEEEECCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]