Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 91211079

GI number: 91211079

Start: 1964196

End: 1965779

Strand: Direct

Name: pykA [H]

Synonym: UTI89_C2058

Alternate gene names: 91211079

Gene position: 1964196-1965779 (Clockwise)

Preceding gene: 91211078

Following gene: 91211083

Centisome position: 38.77

GC content: 51.96

Gene sequence:

>1584_bases
GTGACGATCGCTAAAAACGATTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCG
TTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCA
GAACTAAAATCGTTACCACGTTAGGCCCGGCAACAGATCGCGATAATAACCTTGAAAAAGTTATCGCGGCGGGTGCCAAC
GTTGTACGTATGAATTTTTCTCACGGCTCGCCTGAAGATCACAAAATGCGCGCGGATAAAGTTCGTGAGATTGCCGCAAA
ACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACCTTTAAAGAAGGCAAAGTTT
TCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAAAGAAAAAGTCGGTATCGAC
TACAAAGGCCTGCCTGCTGACGTTGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCGTCCAGTTAAAAGTACTGGA
AGTCCAGGGCATGAAAGTGTTCACCGAAGTGACCGTCGGTGGTCCCCTCTCCAACAATAAAGGTATCAACAAACTTGGCG
GCGGTTTGTCGGCTGAAGCGCTGACCGAAAAAGACAAAGCAGACATTAAGACTGCGGCGTTAATTGGCGTAGATTACCTG
GCTGTCTCCTTCCCACGCTGCGGCGAAGATCTGAACTATGCCCGTCGCCTGGCACGCGATGCAGGATGTGATGCGAAAAT
TGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGAGGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAA
TGGTTGCTCGCGGCGACCTCGGTGTGGAAATTGGCGATCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCG
CGTCAGCTTAACCGCGCGGTAATCACTGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGA
AGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTGATGCTGTCGGCAGAAACTGCTGCTGGTCAATACC
CGTCAGAAACTGTTGCAGCCATGGCGCGCGTTTGCCTGGGGGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGT
CTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCGGCTAACCACCTGAAAGGCGTTAC
GGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCA
TGTCACGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGCGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGAC
GGCGTAGCGGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCAC
CCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAGTAA

Upstream 100 bases:

>100_bases
GGATTTACTTTGCGGCGCGGTACAAAATTCAGAGATAACTTGAAGCGGGTCAAGGAAGCGCTGAAGGAATCGCGTTTTGA
TAAGCAGTTACTTAATTTAA

Downstream 100 bases:

>100_bases
GTACGTTGCCGGATGCGGCGAAAACGCCTCATCCGGCCTACAGTTCAATGATAATTCAACAGATTTCGAATCTTCTGAAG
CAAACTTGAACTTATCATCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTIAKNDCHCPNLIRHPNEINLSPSRRYFIRISCSSNTWLFQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGAN
VVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGID
YKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKADIKTAALIGVDYL
AVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRA
RQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHR
LDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSAND
GVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE

Sequences:

>Translated_527_residues
MTIAKNDCHCPNLIRHPNEINLSPSRRYFIRISCSSNTWLFQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGAN
VVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGID
YKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKADIKTAALIGVDYL
AVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRA
RQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHR
LDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSAND
GVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
>Mature_526_residues
TIAKNDCHCPNLIRHPNEINLSPSRRYFIRISCSSNTWLFQSTEYYMSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANV
VRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDY
KGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKADIKTAALIGVDYLA
VSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRAR
QLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRL
DVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDG
VAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=499, Percent_Identity=36.6733466933868, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286420, Length=499, Percent_Identity=36.6733466933868, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=37.1717171717172, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=34.8884381338742, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=34.8884381338742, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=35.8620689655172, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=35.8620689655172, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.8912133891213, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.8912133891213, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=99.7916666666667, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=488, Percent_Identity=36.8852459016393, Blast_Score=279, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=36.40081799591, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=37.246963562753, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=37.246963562753, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=37.246963562753, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=37.246963562753, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=491, Percent_Identity=36.2525458248472, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=499, Percent_Identity=34.6693386773547, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.246963562753, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.246963562753, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648966, Length=418, Percent_Identity=36.1244019138756, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.1792260692464, Blast_Score=225, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24646914, Length=311, Percent_Identity=34.0836012861736, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 56975; Mature: 56844

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAKNDCHCPNLIRHPNEINLSPSRRYFIRISCSSNTWLFQSTEYYMSRRLRRTKIVTT
CCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHEEEEE
LGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQG
ECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCC
PKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGR
CEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCC
VQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKADIKTAALIGVDYL
EEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHH
AVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQEAMDDIILASDVVMVARGDL
EEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
GVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTD
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
AVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAA
EEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
NHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSAND
HHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEECCCCEEEECCCCC
GVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
CHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
TIAKNDCHCPNLIRHPNEINLSPSRRYFIRISCSSNTWLFQSTEYYMSRRLRRTKIVTT
CCCCCCCCCCHHHCCCCCCCCCCCCEEEEEEEECCCEEEEECHHHHHHHHHHHHEEEEE
LGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQG
ECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCC
PKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGR
CEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCC
VQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKADIKTAALIGVDYL
EEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHH
AVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQEAMDDIILASDVVMVARGDL
EEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
GVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTD
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
AVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAA
EEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
NHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSAND
HHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEECCCCEEEECCCCC
GVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
CHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]