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The map label for this gene is yebU [H]

Identifier: 91211057

GI number: 91211057

Start: 1946907

End: 1948352

Strand: Direct

Name: yebU [H]

Synonym: UTI89_C2036

Alternate gene names: 91211057

Gene position: 1946907-1948352 (Clockwise)

Preceding gene: 91211056

Following gene: 91211058

Centisome position: 38.43

GC content: 52.49

Gene sequence:

>1446_bases
ATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTC
TTTTGATGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAACCTCCGTTGCTG
ATTTCCTGCAATTAACTGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGC
GACAGTGAAGATGCATTGCCGTTGGGTAGTACCGCCGAGCATTTAAGCGGCCTGTTTTATATTCAGGAAGCCAGTTCAAT
GTTGCCTGTTGCCGCCTTGTTTGCTGACGGTAATGCACCACAGCGGGTGATGGATGTCGCTGCTGCACCAGGTTCCAAAA
CGACGCAAATTGCTGCGCGGATGAATAACAAAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTA
CATGCCAATATCAGCCGCTGTGGCATCAGTAATGTTGCGCTCACGCATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCC
AGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCCGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAA
ACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCGACCCAACGGGAGCTGATCGACAGCGCCTTTCATGCATTACGTCTT
GGCGGTACGCTGGTTTACTCGACCTGTACCTTAAACCGGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTA
CCACGACGCAGTAGAGTTTTTACCGCTTGGCGATCTCTTCCCTGGTGCAAATAAGGCGCTGACCGAAGACGGCTTTTTGC
ATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATCCCCGTCTTACCC
GCCCCAAAATACAAAGTGGGCAATTTCCCGTTTAGCCCGGTGAAAGATCGCGAAGCCGGTCAAATTCGTCAGGCGGCTGC
AAGTGTTGGCTTAAACTGGGATGAAAACCTGCGACTCTGGCAGCGTGACAAAGAACTGTGGTTGTTCCCGGTAGGCATTG
AAGCCCTGATCGGTAAAGTCCGATTTTCTCGCTTGGGGATTAAACTTGCCGAAACGCACAACAAAGGTTATCGCTGGCAG
CATGAAGCCGTTATTGCCCTTGCCTCCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGGAAGCGGAAGAGTGGTA
TCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCAGCATCAGCCGATTGGTT
TAGCCAAACGGATTGGTTCACGACTGAAAAACAGCTACCCGCGTGAACTGGTGCGCGACGGGAAACTTTTTACCGGTAAC
GCCTGA

Upstream 100 bases:

>100_bases
GAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCC
TGTTTTTTTGCCAGTGGTAT

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGACTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAATCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIER
DSEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRL
GGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGDLFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A

Sequences:

>Translated_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIER
DSEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRL
GGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGDLFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A
>Mature_481_residues
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIER
DSEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVL
HANISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRL
GGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGDLFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLP
APKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQ
HEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
A

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=305, Percent_Identity=36.7213114754098, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI76150623, Length=305, Percent_Identity=36.7213114754098, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=37.7245508982036, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI39995082, Length=376, Percent_Identity=26.063829787234, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=154, Percent_Identity=35.0649350649351, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI23199998, Length=359, Percent_Identity=27.8551532033426, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI270288816, Length=329, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI270288818, Length=324, Percent_Identity=28.3950617283951, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI8922322, Length=324, Percent_Identity=28.3950617283951, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI40316918, Length=210, Percent_Identity=29.5238095238095, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=97.286012526096, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=319, Percent_Identity=34.4827586206897, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25143471, Length=287, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=26.7080745341615, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=28.82096069869, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=290, Percent_Identity=38.2758620689655, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=32.0388349514563, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI22024126, Length=311, Percent_Identity=35.6913183279743, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355201, Length=207, Percent_Identity=35.2657004830918, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=34.8571428571429, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=34.8571428571429, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53450; Mature: 53450

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEHHHHHHHHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCHHEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRL
EHHHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
GGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGDLFPGANKALTEDGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEE
DCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDENLRLW
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTP
ECCCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C
>Mature Secondary Structure
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKTSVADFLQLTA
CEEECEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEHHHHHHHHHHHHHHHC
PYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
CCCCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCH
QRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
HHHHHHHCCCCCCHHEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCE
VFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRL
EHHHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
GGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGDLFPGANKALTEDGFLHVFPQIY
CCEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEE
DCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDENLRLW
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHE
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALASPDNVNAFELTP
ECCCCEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEEEEEECCCCCCEEEECH
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGN
HHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA