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The map label for this gene is emrB [H]

Identifier: 91210633

GI number: 91210633

Start: 1521426

End: 1522940

Strand: Direct

Name: emrB [H]

Synonym: UTI89_C1610

Alternate gene names: 91210633

Gene position: 1521426-1522940 (Clockwise)

Preceding gene: 91210632

Following gene: 91210634

Centisome position: 30.03

GC content: 41.85

Gene sequence:

>1515_bases
ATGATGTCTATGCGTAAACATATTGCTTTCGCCAGTATGTGTATTGGATTATTTATTGCTCAGCTTGATATTCAAATTGT
ATCGTCATCCCTAAATGAAATAGGTGGTGGTCTGTCCGCAGGTAAAGACGAAATGGCCTGGCTTCAGACCAGCTATTTAA
TTGCCGAGATTATTGTTATTCCGCTCTCTGGGTGGCTGTCACGGGTATTTTCCACTCGTTGGCTTTTTACTCTATCCGCG
GGAATATTTACTTTAATGAGTATCGCTTGTGGACTTGCATGGAATATTCAGATCATGATTTTTTTTCGTGCTTTACAGGG
AGTTGCTGGCGCATCCATGATCCCTTTAGTTTTCACGACGGCGTTTATTTATTACCAGGGTAAAGAGTTGGGGTTGGCTG
CTGCGGTAGTCAGTGCGCTGGCCTCACTGTCACCGACGCTAGGGCCAACTCTGGGGGGCTGGATCACTGATAACCTCGAC
TGGCGATGGCTATTTTATATCAATATATTACCGGGCATATATTTAGTTTTGAGTATTCCTTTTCTGGTTAATTTTGATAA
ACCTGATCTCTCTTTACTGAAAGTTGCTGATTATCCGAGTATTATCCTGTTGGCCATGACACTGGGATGTCTGGAATATA
CCCTTGAAGAAGGAGCGCGCTGGGGATGGCTGGATGATAATACTATTTTGCTGACATCAGTTCTGGCATTGGTGAGTTTT
ATTTTATTTGCTGCGCGTACACTTAAAATATCCAATCCGATAATGGATCTTCATGCTTTTAAGGATAAATATTTCACACT
GGGATGTTTCTTTTCTTTTTCTGGAGGGGTTGGAATATTCTCTACTGTATATTTAATACCTGTGTTTTTGGGGCAGGTTC
GCGGATTAAATGCCGAAGAGATAGGTTTTGCCGTTTGCACAACAGGTATTTTCCAGTTATTTTCTGTTCCCTTTTACTTC
TGGTTAAGTAAAAAAATAAATCTGCAATGGCTGTTGATGGCGGGGCTGGGCGGATTTGTGTTTTCAATGTATCTTTTTAC
CCCTATAACCCACGAATGGGGATGGCAGGAGTTACTGTTTCCACAGGCAATTAGAGGGATTTCGCAGCAGTTTGCGATGG
CCCCCATCGTAACATTAACGCTGGGGGGAATTCCAAAAGAACGGCTGAAGTTAGCCTCTGGTGTATTTAATTTAACACGT
AATCTTGGGGGCGCAAGTGGTATTGCCCTGTGTGGCTCTATTCTCAATAATCGAACTAATTTTCATTTTTCGAGAATGGG
TGAAAAAATGGTTTCTGTTCCCCATACGATGAATGACTTCATTTCCAGAAGTGCTTTGTTTTTTAATCGCAGTGGAAGTG
ACCAGACATCGGAAATACTGGCTTCAACCAAATTATTGAGTCAATTGATGCTGAGGGAGGCGCAAACTATGGCATTTTCG
GATACCTTTCTGCTAATCAGTGGTCTGTTATTTATCGCATTTTTGTTGGTTCCTGCTATGAATAAATCATCCTGA

Upstream 100 bases:

>100_bases
TTGTTCAGCGTGTCCCGGTACGGATTGCATTTGACGACGCAAAGGAATTAAAGCAACTTCTTCGCCCGGGACTGTCCGTC
ACCGTCAGTGTTGATGAGAG

Downstream 100 bases:

>100_bases
TCGGAGCCTGTGCTGAATTAAACTCGGGCTAAACTGTTCGATACTGCTAACAGGCTAGTATAAAATGCCCCGCCTGCGCA
GATACTCACAGGTATGAAGT

Product: transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLKISNPIMDLHAFKDKYFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYF
WLSKKINLQWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGASGIALCGSILNNRTNFHFSRMGEKMVSVPHTMNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS

Sequences:

>Translated_504_residues
MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLKISNPIMDLHAFKDKYFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYF
WLSKKINLQWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGASGIALCGSILNNRTNFHFSRMGEKMVSVPHTMNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS
>Mature_504_residues
MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSA
GIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
ILFAARTLKISNPIMDLHAFKDKYFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYF
WLSKKINLQWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTR
NLGGASGIALCGSILNNRTNFHFSRMGEKMVSVPHTMNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
DTFLLISGLLFIAFLLVPAMNKSS

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=425, Percent_Identity=29.6470588235294, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788710, Length=429, Percent_Identity=29.1375291375291, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=26.056338028169, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=24.822695035461, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788392, Length=414, Percent_Identity=24.8792270531401, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=194, Percent_Identity=25.2577319587629, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55611; Mature: 55611

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
PLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRWLFYINILPGIYLVLSIP
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHH
FLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
HHEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ILFAARTLKISNPIMDLHAFKDKYFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEE
HHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHH
IGFAVCTTGIFQLFSVPFYFWLSKKINLQWLLMAGLGGFVFSMYLFTPITHEWGWQELLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGASGIALCGSILNNRTN
HHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
FHFSRMGEKMVSVPHTMNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
CHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFLLISGLLFIAFLLVPAMNKSS
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
PLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLDWRWLFYINILPGIYLVLSIP
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHH
FLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSF
HHEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ILFAARTLKISNPIMDLHAFKDKYFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEE
HHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHH
IGFAVCTTGIFQLFSVPFYFWLSKKINLQWLLMAGLGGFVFSMYLFTPITHEWGWQELLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGASGIALCGSILNNRTN
HHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
FHFSRMGEKMVSVPHTMNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFS
CHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DTFLLISGLLFIAFLLVPAMNKSS
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]