Click here to switch to the map view.

The map label for this gene is rusA [H]

Identifier: 91210503

GI number: 91210503

Start: 1401764

End: 1402138

Strand: Direct

Name: rusA [H]

Synonym: UTI89_C1478

Alternate gene names: 91210503

Gene position: 1401764-1402138 (Clockwise)

Preceding gene: 91210501

Following gene: 91210504

Centisome position: 27.67

GC content: 51.73

Gene sequence:

>375_bases
ATGCTCATAGATTTGGTTTTACCTTACCCGCCGACGGTGAACACTTACTGGCGACGCCGTGGCAGCACATATTTTATCTC
GGAGGAGGGAAAGCGTTATCGCCGGGCTGTGGCGCTTATTGTTCGCCAGCAGCGGCTGAAATTAAGCCTGTCCGGAAGGC
TGGCGATAAAGGTGATTGCAGAGCCACCGGATAAGCGTCGTCGCGACCTGGACAATATCCTGAAAGCACCGCTGGATGCG
CTGACGCATGCGGGAGTGTTAATGGACGATGAGCAGTTTGATGAAATCAATATCGTTCGTGGTCAGCCAGTATCTGGTGG
ACGTATGGGGGTGAAGATTTACCCCATAATGCATGAAGAGCAGGTCAAAAAATGA

Upstream 100 bases:

>100_bases
TCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACTGGCGTGC
TTGGGTAAAAGAGGTGACTG

Downstream 100 bases:

>100_bases
AACTGGAAGATTTACCGAAATACTACTCCCCAAAATCCCCTGGCCTGACCGATGCATCGGCCTCAACGTCAAAAGATGCG
CTGAGTATCACTGATGTGAT

Product: endodeoxyribonuclease RusA

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRMGVKIYPIMHEEQVKK

Sequences:

>Translated_124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRMGVKIYPIMHEEQVKK
>Mature_124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDA
LTHAGVLMDDEQFDEINIVRGQPVSGGRMGVKIYPIMHEEQVKK

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=115, Percent_Identity=38.2608695652174, Blast_Score=87, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14298; Mature: 14298

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIA
CEEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRMGVKIYPIMHEE
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECHH
QVKK
HHCC
>Mature Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIA
CEEEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRMGVKIYPIMHEE
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCCCCEEEEEEECHH
QVKK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA